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The objective of this study was to determine the effect of using Bracket Oliphant medium (BO) extender at different temperatures on motility, viability, abnormalities and plasma membrane integrity of sexed sperms using fresh semen of Sumba Ongole (SO). Sexing sperms was carried out using a BO extender in the BSA column. The parameters observed in this study were motility, viability, abnormalities and plasma membrane integrity and were compared using the Paired-Sample t-Test with two treatments and six replications. P1 was BO extender with a temperature of 27oC and P2 was BO extender with a temperature of 37oC. The results of this study showed that there were no significant different (P>0.05) between the two treatments of the sperms X for motility (65.8%, 53.3% and 41.3% vs. 66.3%, 55.0% and 43.3%), viability (65.9%, 68.0% and 67.0% vs. 67.7%, 61.9% and 69.6%) and plasma membrane integrity (70.1% and 59.8% vs. 64.9% and 64.2%) after sexing, equilibration and thawing, but differed significantly (P<0.05) in abnormalities after sexing (15.5%, 15.6% and 16.2% vs. 15.5%, 17.3% and 19.5%). Likewise, the motility (66.7%, 55.8% and 42.1% vs. 65.8%, 55.0% and 42.1%), viability (68.8%, 59.0% and 64.5% vs. 67.4%, 59.4% and 71.7%), abnormalities (13.6%, 19.4% and 16.6% vs. 18.9%, 18.6% and 17.2%) and plasma membrane integrity (68.1% and 57.5% vs. 62.8% and 62.8%) of sperms Y at P1 and P2 did not differed significantly (P>0.05). The difference in the value of abnormalities after sexing is still within the limits of abnormalities of the sperms. It can be concluded that the use of the BO extender method can be used at a temperature of 27oC and at 37oC in the process of sexing sperms.