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Cyclodextrins are a family of cyclic oligosaccharides produced by enzymatic degradation of starch. There are three types of cyclodextrins based on the number of glucose subunits, i.e., α-cyclodextrin, β-cyclodextrin, and γ-cyclodextrin, which respectively have 6, 7, and 8 glucose subunits. Among others, β-cyclodextrin has more advantages in many subjects. This paper provides the development of β-cyclodextrin particles using electrospraying method. In this study, a variation of β-cyclodextrin concentration was carried out to see the effect on the particle diameter. By varying the β-cyclodextrin concentration, the resulting particles have various sizes. The greater the β-cyclodextrin concentration, the greater the diameter of the particles produced. β-cyclodextrin particles can be used in food, pharmaceutical, drug delivery, and chemical industries. The advantage in modifying the particle diameter makes the use of β-cyclodextrin more effective.