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The purpose of this study was to determine the molecular weight of the protein STAT STAT 5a and 5b that exist in liver tissue as a basis to determine the amino acid composition of broiler STAT protein phosphatase that is experiencing growth due to increased growth hormone (GH). Broiler liver tissue samples isolated from broilers were maintained for 21 days, then the examination followed by SDS Page and Western Blott. Results Western Blott known that STAT 5a protein molecular weight of 91 kDa with an amino acid composition ciggppkvmnmeesn or cysteine, isoleucine, glycine, glycine, proline, proline, lysine, valine, methionine, asparagine, methionine, glutamate, glutamate, serine, asparagines and STAT 5b of 90 kDa with an amino acid composition datnilvspvylypdip or aspartate, alanine, threonine, asparagine, isoleosine, leucine, valine, serine, proline, valine, tyrosine, leucine, tyrosine, proline, aspartate, isoleucine, proline.