Indonesia Conference Directory


<< Back

Benzyl Amino Purine Enhances Multiplication of Vanda tricolor Protocorm Like Bodies
Innaka Ageng Rineksane, Evo Wandi Saputra, Gatot Supangkat, Agung Astuti

Universitas Muhammadiyah Yogyakarta, Indonesia


Abstract

Vanda tricolor orchids are endemic to the slopes of Mount Merapi, Yogyakarta Province, Indonesia. The mountain slopes and eruptions in 2006 have destroyed 80% of the habitat and threatened the existence of these orchids. In addition, the exploitation of Vanda tricolor out of its natural habitat by the community for collection or selling it outside the area has reduced the orchid population. Therefore, efforts should be carried out to improve technology to reproduce and regenerate Vanda tricolor orchids. One of alternative to regenerate Vanda tricolor is using multiplication technique through in vitro and ex vitro cultures. The objective of this study was to determine the best type and concentration of cytokinin for the multiplication of Vanda tricolor PLBs. The experiment was arranged in a completely randomized design with single factor consist of 7 treatments id est medium without cytokinin, BAP 0.5 mgL-1, BAP 1 mgL-1, TDZ 0.5 mgL-1, TDZ 1 mgL-1, Kinetin 0.5 mgL-1 and Kinetin 1 mgL-1. Parameters observed in this study were a percentage of live, browning and contamination explant, the diameter of PLB, the emergence of shoots, the percentage of explant sprouting, the number of buds, the emergence of roots. The data were analyzed by using The Analysis of Variance at α=5% and further tested using Duncan Multiple Range Test (DMRT) at α=5%. The results showed that BAP 0.5 mg / L was the best cytokinin for multiplication of Vanda tricolor PLBs.

Keywords: Vanda tricolor,Tissue Culture, Cytokinin, Multiplication

Topic: International Conference on Sustainable Agriculture

Link: https://ifory.id/abstract/m6fZqyGF4Vxz

Conference: The 3rd International Conference on Sustainability and Innovation (ICoSI 2019)

Plain Format | Corresponding Author (Siti Nur Aisyah)

Featured Events

<< Swipe >>
<< Swipe >>

Embed Logo

If your conference is listed in our system, please put our logo somewhere in your website. Simply copy-paste the HTML code below to your website (ask your web admin):

<a target="_blank" href="https://ifory.id"><img src="https://ifory.id/ifory.png" title="Ifory - Indonesia Conference Directory" width="150" height="" border="0"></a>

Site Stats