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Abstract Objective: The objective of this study was to determine the effectivity of Haruan fish (Channa Striata) extract which added calcium hydroxide to ALP levels in the odontoblast cell line. Methods: The study used a posttest only control group design. Odontoblast MDPC-23 cell line which distributed in 96-well microplates is divided into 8 groups. ALP levels were measured by ELISA kit. Data were analyzed statistically using ANOVA statistical test, followed by Bonferronis post-test. Results: The ALP level in the odontoblast cell line in Haruan fish extract that added with calcium hydroxide increased compared to the control. At a concentration of 25 µg/ml = 208.37 µg/ml, a concentration of 50 µg/ml = 219.04 µg/ml, a concentration of 100 µg/ml = 282.93 µg/ml and differed significantly from the control group. Conclusion: The effect of Haruan fish extract added with calcium hydroxide increase the ALP level in odontoblast cell line as concentration increases.