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Abstract Objective: To determine and compare measurement results of setting time and viscosity on alginate impression materials made of brown algae Sargassum sp. and Padina sp Methods: This study was an observational study with cross sectional design. Samples were sodium alginate extracted from brown algae Sargassum sp. and Padina sp. obtained from Punaga waters, South Sulawesi. Sample was formulated into irreversible hydrocolloid impression material, then measured for its setting time and viscosity. Results: Setting time of impression material made of Sargassum sp was 5.30 while that of Padina sp was 4.40. Based on ANSI / ADA No. 18/1992, these results were classified as alginate regular setting type. Viscosity of impression material made of Sargassum sp was 9760 while that of Padina sp was 9200 cps. Based on SIGMA 2008, these results were classified between medium and high viscosity types. Conclusion: Brown algae Sargassum sp. and Padina sp. has the potential to be one of the basic ingredients for manufacturing dental impression materials.