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Cigarette residues can accumulate in semen and sperm thereby reducing sperm quality and causing infertility in men. Meanwhile, lycopene in tomatoes (Solanum lycopersicum) is an antioxidant that has protective mechanism against oxidative stress, acts as an anti-lipid peroxidation, and involves in regulating gene function through non-oxidative mechanisms. Therefore, this study was conducted to determine the effect of tomato juice administration in improving sperm quality in mice exposed to third-hand smoke. This research was experimental study with a completely randomized design. Seventeen adult male mice (8-10 weeks in age, 35-40 g in weight) were randomly divided into three groups, the control group (C) and two treatment groups (T). Treatment group 1 (T1) was exposed to tertiary cigarette smoke for 14 days and treatment group 2 (T2) was exposed to tertiary cigarette smoke and received tomato juice for 14 days. After the treatment period completed, the animal was sacrificed by cervical dislocation. Spermatozoa were taken from the cauda epididymis, sperm quality examination was done immediately. Parameters measured in sperm quality examination were number, morphology, and motility of the spermatozoa. The results showed that number of the sperm in T1 tended to be lower than that in the control group and T 2, but this difference did not differ significantly (p> 0.05). While abnormal sperm morphology in the test group was significantly higher than that in the control group (p <0.05). These results indicate that tomato juice has potential in improving sperm quality.