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The objective of research is to explain the influence of variables Understanding of Organization Culture, Work Motivation, and Work Ability on Employee-s Performance simultaneously and partially. Research type is explanatory research. The samples studied were 70 respondents with compositions made up of employees and agents of PT Asuransi Jiwasraya Branch Office Malang. Data analysis uses descriptive analysis and multiple linier regression analysis supported by SPSS for Windows ver 17.0. Based of the hypothesis testing result, it was found that variables Understanding of Organization Culture, Work Motivation, and Work Ability showed simultaneously and partially have a significant effect on Employee-s Performance. Based on those result of this research, it is suggested that the company be able to maintain and improve the Understanding of Organization Culture, because this variable have big contribution of Employee-s Performance. Therefore, it is supposed that this research have many positive contribution for PT Asuransi Jiwasraya Branch Office Malang and it can make improvement of Employee-s Performance significantly.