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The method of measuring the absorption of carbon dioxide by a chamber at this time known only by many researchers, so it necessary to explains the stages of conducting research by the chamber method. The chamber method is used to measure the absorption of carbon dioxide (CO2), especially in the growth rate of seedlings or tillers. The purpose of this scientific paper is to explain the procedure for measuring CO2 absorption by the chamber method at the seedling or sapling growth rate. In the early, chamber method used to measure Greenhouse Gas (GHG) emissions on peatlands, but follow to the development of technology, this methods are also used to measure CO2 uptake in plants, especially at seedling level. Size of the chamber for measuring CO2 absorption was 50x 50x30 cm equipped by a thermometer, fan and dry battery. Samples of CO2 gas takes from the chamber by syringe (size 10 ml). Equipment for analyzing CO2 gas samples, namely Gas Cromatography (GC). The results of measurements of CO2 absorption by the chamber method are easier and simpler than another method. Data obtained can be used to estimate CO2 absorption of seedling growth level. Because the limited of the chamber size, it can measure CO2 absorption only at seedling growth level.