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Introduction: Diabetes is caused one them by inflammatory regulation disorders. Metformin has been reported that inhibits the physiological function of normal cells. Asiatic acid, a bioactive compound from centella asiatica has the potential to be developed as a therapeutic agent for diabetes, but little is known about its toxic effects on macrophage cells and fibroblast cells. Objective:The aims of study is evaluate the toxic effects of asiatic acid and metformin on the viability of RAW264.7 macrophage cells and NIH3T3 fibroblast cells. Method: Asiatic acid and metformin with seven concentrations were given to RAW264.7 macropage cell lines and NIH3T3 fibroblast cell lines. Viability percentage is calculated using the 3- (4,5-dimethyltiazol-2-il) -2,5-diphenyltrazolium bromide method and the absorbance is measured at 595nm. Results: Results have shown that asiatic acid with concentrations> 12.5 μg / mL decreases the viability of RAW264.7 and NIH3T3 cells drastically. RAW264.7 and NIH3T3 cells that had been given metformin concentrations of 11.6 μg / mL to 370 μg / mL still showed a large percentage of cell viability. Conclusion: Asiatic acid has shown that the cytotoxic effect is greater than metformin, so it is necessary to pay attention to the concentration of the treatment.