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Practicum guides are one of the important teaching materials. The provision of appropriate practicum guides and in accordance with existing needs is needed, in order to support the learning process of students. This study aims to analyze biomolecular practicum guides used by students of the chemical education program at Unimed. The method in this research is descriptive analysis, using a practicum guidance analysis questionnaire given to three expert lecturers. The average analysis results obtained are 2.7; 2.65; 2.5. Based on the results of the analysis it was found that biomolecular practicum guides used by students of the chemical education program Unimed were still not in accordance with the KKN curriculum, for that in further research it was necessary to develop biiomolecular practicum guides that were in line with KKNI curriculum.