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Background and purpose : Iodine is reported has antineoplastic effect on tumour. This study investigate the proliferation effect of three types of breast cancer cell lines after iodine (I2) , lugol and combination of both treatments. Materials and Methods : We used MCF7, SKBR3 and MDAMB 231 cell lines which represented luminal A, HER2+ and triple negative breast cancer subtypes. Those cells were treated with I2, lugol and combination of both. Cells proliferation was assed using colorimetric assay. Results : Cell proliferation rate of MDA MB321 cell which received combination of I2 and lugol with doses 1%, 0,1%, 0,01% are 2% ± 0%, 9% ± 1%, 10% ± 0% respectively. The treatment reduced cell proliferation significantly p<0.05 compare to I2 or lugol alone. Cell proliferation rate of MCF7 cell which received 20 nM, 40nM, 60nM of I2, lugol and combination of both are 39% ± 8%, 19% ± 8%, 11% ± 8%, 80% ± 59%, 8% ±10%, 11% ± 7%, 29% ± 15% respectively. Those treatments reduced cell proliferation rate in MCF7 significantly P<0.05. Iodine and lugol treatments in these doses show increase proliferation rate trend in SKBR3 cells. Conclusions: Iodine treatment reduced cell proliferation rate in triple negative and luminal A cell lines. Iodine might be involved in the pathogenesis of breast cancer cells depend on doses and types of cells. Further studies are needed to investigate the role of iodine in breast cancer.