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Electronic learning (e-learning) is becoming popular among students and lecturers in higher education. e-learning provides new opportunities for sharing knowledge and interacting between students and lecturers. Therefore, this study aims to examine the impact of design of learning contents on the acceptance of students using new technology as a tool for their learning medium. Based on the Technology Acceptance Model (TAM), which emphasizes on perceived usefulness, perceived ease of use and attitude toward e-learning, is used to measure the factor of acceptance the technology for e-learning in higher education. The study empirically validated the model using data collected from a survey to university students in Airlangga University, Surabaya. Partial Least Square is used to examine the research model. The results show all of the indicators are valid and design of learning contents is an important of variable and influences significantly on the two main variables of TAM, perceived usefulness and perceived ease of use. From the study, perceived usefulness and perceived ease of use have a positive impact on attitude toward e-learning.