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This study was to know induction of protective immunity that represented endogenous development of parasites and oocyst production using some low doses of E. tenella oocysts through challenged test. The present study used 55 broiler chickens at 3 weeks old were divided into 4 groups. Group 1 was 10 chickens administered aquades, after 14 days administered aquades then challenged 5 x 103 E. tenella oocysts, 4 days post challenge 5 chickens were sacrificed to observe morphological endogenous development of parasites by histopathological changes examination, while oocysts production was calculated on 5 other chickens from day 6 to 12 post challenge. Group 2, 3 and 4 which each group consisted 15 chickens inoculated 1 x 102, 2 x 102, 3 x 103 E. tenella oocysts respectively, 4 days pi 5 chickens were sacrificed to observe morphological endogenous development of parasites by histopathological changes examination, while oocysts production was calculated on 5 other chickens from day 6 to 12 post for primary infection. Fourteen days after primary infection, each group (2, 3 and 4) were challenged 5 x 103 E. tenella oocysts, 4 days post challenge 5 chickens were sacrificed to observe morphological endogenous development of parasites by histopathological changes examination, while oocysts production was calculated on 5 other chickens from day 6 to 12 post challenge. The results showed that group 1, in challenged test, parasites were development and proliferation well so that oocyst production clearly increased, whereas group 2, 3 and 4 were seen few development and proliferation of parasites, there were no significant oocysts production around group. In conclusion, the endogenous development disabilities of parasites occur as a result of protective immunity generated resulting from the first antigen exposure so that proliferation and multiplication of parasites became decreased.