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Lung cancer is a cancer with the highest incidence in the world. Conventional chemotherapy for lung cancer has side effects, high resistance and toxicity, so it is necessary to search for sensitive and effective chemotherapy drugs from natural ingredients that are widely Tersedia di in Indonesia. This study aims to examine the anticancer effect of compound 1,2-epoxy-3 (3- (3,4-dimethoxyphenyl) -4H-1-benzopiran-4on) propan (EPI) which is synthesized from clove leaf oil and its combination effects with Doxorubicin on HTB183 lung cancer cells. This research is a pure in vitro experimental study of HTB183 lung cancer culture cells. Toxicity tests were carried out using the method of tetrazolium 3- (4,5-dimethylthiazol-2-il) 2,5-diphenyltetrazolium bromide (MTT) to calculate cell viability. IC50 value was obtained by analysis using probit regression calculation using SPSS software. The synergism of this compound with Doxorubicin was determined based on the value of the Combination Index (IK) using a combination test with series 1/2IC50, 3/8IC50, 1/4IC50, and 1/8IC50 and the data was analyzed using Compusyn 1.0 software. The results showed IC50 EPI compounds is 1.41(±0.86)μg/mL, and Doxorubicin is 2.21(±0.98)μg/mL. The IK value of EPI-Doxorubicin has an average value of 0.3-0.7 which means it has a synergistic effect, and the lowest value is obtained from a combination of EPI 0,63μg/mL and Doxorubicin 0,26μg/mL with CI = 0.066. In conclusion EPI compounds have anticancer effects potent to HTB183 lung cancer cells and synergistic with Doxorubicin.