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Titanium dioxide (TiO2) nanoparticles (NPs) have been successfully synthesized using the green synthesis method, which used peperumia pellucida leaf extract as reducing agent and TiCl3 as precursor. The prepared TiO2 NPs were characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS) and ultra violet visible (UV-Vis) spectroscopy. XRD result shows that the NPs are in nature anatase phase at the angles of 2 theta 25.3o, 37.54o, 49.91o, 53.84o and 62.72o corresponding to crystal orientations (101), (004), (200), (105) and (204). Based on SEM image, the sizes of these materials are in the range 20-40 nm. The maximum optical absorbance and calculated band gap energy are around 202 nm and 3.68 eV, respectively.