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Long term application of anorganic fertilizers had damaged the soil physically and chemically, thus reducing the soil support capacity toward the plants growth. It also suppressed the population of beneficial microbes in the soil. The use of organic fertilizers made from cow blood and bones in combination with coconut fiber ash might improve the plant productivity without causing severe damage towards the soil. Aim of this study was to analyze the macronutrients (nitrogen, potassium and phosphorus) content resulted from various combination ratios of cow blood, cow bones and coconut fiber ash. This study consisted of two main steps, including the making of organic fertilizer and analysis of macronutrients content. Organic fertilizer was composed using five different ratios in the combination of cow blood (4.0-6.0 g), cow bone (1.75-2.75 g) and coconut fiber ash (4.0-6.0 g). Results showed that each combination (cow blood, bone and coconut fiber ash) produced various level of macronutrients content. The highest nitrogen content (1.74%) was achieved from the combination of 5.5 g blood, 2.5 g bone and 5.5 g coconut fiber ash. The highest content of available phosphorus (P) (41.469 ppm) was produced by the combination of 5.0 g blood, 2.25 g bone and 5.0 g coconut fiber ash. The highest content of available potassium (154.25 Cmol/kg) was obtained from the combination of 4.0 g blood, 1.75 g bone and 4.0 g coconut fiber ash.