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Knowledge about the importance of stretching, flexibility and PNF is still lack. In fact, there are a lot of things which can be gained from doing those activities in exercising, for example avoid injury while practicing and reducing or preventing excessive fatigue after exercise. This research purposed to find out PNF effect towards the muscle flexibility of members fitness centre in The Fitlab. The type of the research was true experimental design with pretest-posttest control group design. This study had two groups: experimental group and control group. The experimental group was treaten with cooling PNF and the control group was untreated. The treatment given by the researcher was 16 sessions with a frequency of four times a week for 4 weeks or a month. The sampling technique in this study uses simple random sampling. Sampling (sample size) uses a formula from Slovin and obtained as many as 24 people. The instrument of the study was the Sit and Reach test. The data analysis used t test (paired sample t test) at the 0.05 level of significance. The survey result revealed that there was significant influence in the experimental group who received PNF treatment to the muscle flexibility of the fitness members compared with the control group that did not receive treatment. From the results, it can be seen that the t value in each group, the experimental group t value (7.528) > t table (2.200), whereas the control group t value (-0.053) > t table (2.200).