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Availability Natural Iron Sand that abundant in Indonesia as the main source of ingredient magnetic is a potential raw material for synthesizing of Mn0.25Fe2.75O4/AC. Preparation of Mn0.25Fe2.75O4 /activated carbon (AC) has been successfully synthesized by using the co-precipitation method. Furthermore, the variations of AC mass in Mn0.25Fe2.75O4/AC filler composition also has been done to find out the effect of nanostructural and magnetic Properties from the contribution of Mn0.25Fe2.75O4/AC that characterizes by using X-Ray Diffraction (XRD) and Vibrating Sample Magnetometer (VSM) instrument respectively. The particles size of Mn0.25Fe2.75O4/AC with the filler of AC 0.1, 0.2, and 0,3 g by using XRD characterization is 7.4, 3.67 and 11.8 nm recpectively. Based on VSM Characterization, the value of Magnetization saturation of Mn0.25Fe2.75O4/AC is 0.77, 0.003, and 0,002 emu.gram-1 with the filler of AC 0.1, 0.2, and 0.3 g respectively.