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Plants used for traditional medicine can be used as an alternative search for new antimicrobial substances. One of the medicinal plants that is utilized optimally by the Indonesian people and has the potential to produce antimicrobial compounds is the vetiver (Vetiveria zizanioides L.). This study aims to carry out phytochemical screening of vetiver extract (V. zizanioides L.), test the antimicrobial activity of vetiver extract (V. zizanioides L.) on the growth of pathogenic microbia Staphylococcus aureus, Escherichia coli, Nigrospora oryzae, Rhizoctonia solani and Curvularia sp., and find out the best concentration of vetiver extract (V. zizanioides L.) in inhibiting the growth of pathogenic microbial S. aureus, E. coli, N. oryzae, R. solani and Curvularia sp.. The results reveal that the vetiver (V. zizanioides L.) contains flavonoid, tannin/phenolic chemicals, saponins, steroids, triterpenoids, and alkaloids. In antimicrobial testing, vetiver extract (V. zizanioides L.) showed maximum activity againts N. oryzae, producing the maximum zone of inhibition 19,8 mm at a concentration of 50 mg/ml .