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This research aims to know the effectiveness of multimedia content based on virtual to improving students critical thinking skills on the concept of human skeletal system for fifth grade students in Primary schools. This research is quantitative reasearch with a quasi experiment type, research design use nonequivalent control groub. Research sample was a population member of 66 fifth grade students in the 2019/2020 school year which were divided into experimental classes and control classes. Data collection techniques in this reasearch used tests and observations. The results of reasearch through descriptive analysis obtained an average of N-Gain in experimental class 56.85 with a maximum value is 92.00 and a minimum value is 19.57 this result interpretation is quite effective and average of N-Gain in control class is 36.25 with a maximum value is 72.58 and a minimum value is 7.41 are included in the ineffective interpretation. Inferential analysis through parametric statistics with Independent Sample T Test shows the value of 1/2sig. (2-tailed) 0,000 <α (0,05) and tcount = 5,032 ≥ ttable = 2,000 so H0 is rejected. The research conclusions is virtual-based multimedia content effective in improving critical thinking skills.