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Background : Streptococcus pneumoniae is a normal nasopharyngeal flora. S. pneumoniae bacteria can colonize the nasopharynx without showing symptoms of a disease or called a carrier. The S. pneumoniae bacteria that colonize the nasopharynx can also move to the middle ear through the eustachian canal and cause acute otitis media disease (OMA). S. pneumoniae has 97 serotypes based on the polysaccharide capsule configuration. Each serotype of S. pneumoniae can cause different diseases and more colonize the nasopharynx of children than in adults. In Indonesia, Nasopharyngeal S. pneumoniae colonization studies are still very limited and the study of colonization of S. pneumoniae in children with OMA over the age of five has not been done. Purpose : This study aims to detect S. pneumoniae carried by the nasopharynx of children with primary school age (6-12 years) in Otitis Media Acute, identify serotypes of S. pneumoniae. Methods : The design of this research is a descriptive study. Sampling was carried out in September 2018 to December 2018 in several Public Elementary Schools in Banyumas Regency. Detection of S. pneumoniae in OMA samples can be done through microbiological identification of S. pneumoniae. Serotype identification was performed by serotyping S. pneumoniae with multiplex PCR technique. Results : This study indicate that the percentage of S. pneumoniae carried by the nasopharynx of in primary school children age 6-12 years with OMA in the Banyumas district is 34.6% (9/26 samples). S. pneumoniae serotypes found in nasopharyngeal samples with OMA patients are 6A / B, 6C / D, 17F, 3, 13, 14, 23B, and untypeable. Conclusion : Streptococcus pneumoniae carried by the nasopharynx of children with Acute Otitis Media aged 6-12 years in the Banyumas district by 34.6%. The S. pneumoniae serotype carried by the nasopharynx is 6A / 6B, 6C / 6D, 14, 17F, 3, 23B, 13, and untypable.