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Anticoagulants are commonly used in hematologic laboratories to thin the blood, anticoagulants normally used by EDTA, Garlic also has a compound that serves as an anticoagulant, but not all anticoagulants can be used because it can affect the morphology of blood cells. The purpose of this study was to compare the erythrocyte morphology using EDTA anticoagulants and garlic filtrates as alternative anticoagulants. This type of research is analytic. The sample was taken by non-random purposive sampling as many as 33 students from the total population of 50 students of semester VIII D IV of Health Analyst University of Muhammadiyah Semarang, then identified the erythrocyte morphology on EDTA anticoagulant samples and garlic filtrate. The result of the examination showed that there were 2 samples of samples using EDTA anticoagulant. There were 2 samples of crenation, while those using garlic 1 filtrate were crenated, this showed that samples using EDTA anticoagulant were slightly more likely to occur than garlic filtrate. Chi square statistic test obtained value of significance of 0.061 with significance level of 0.05 that is> 0.05 so it can be concluded that there is no difference of erythrocyte morphology using EDTA anticoagulant and garlic filtrat as alternative anticoagulant.