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Complex Vertebral Malformation (CVM) is a genetic defect due to an autosomal recessive gene in dairy cows that causes anatomic deformation especially in the cervix and thorax in the spine, rib reduction and bone shortening in the front legs. The purpose of this study was to identify the distribution of recessive alleles from CVM in the dairy cow population in Enrekang District using the PCR-PIRA method. A total of 80 DNA samples collected from blood samples from FH dairy cattle from the Enrekang District Animal Husbandry Center (50 samples from Cendana District and 30 samples from Angeraja District) were extracted and amplified by PCR-PIRA technique, then PCR products were cut with the Pst1 restriction enzyme. The identification of the CVM carrier allele is calculated based on the genotype frequency and the allele. The results obtained from this study were that there were no CVM recessive alleles found either in Cendana District or in Angeraja District. The results of this study concluded that there were no dairy cows with carier genotypes (heterozygotes) in the dairy cow population in Kab. Enrekang, with normal CVM frequencies fixed on allele C (normal cattle) (100%).