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The emerging Portable Device and Electrical Vehicle require safe, portable, and high-power energy sources which may be supplied by Lithium Ion Battery (LIB). The currently available carbon anode in LIB’s system posseses some safety issue such as volume expansion during lithiation/delithiation and formation of solid electrolyte Interphase (SEI) that can be harmful for battery. Accordingly, anode material Li4Ti5O12 (LTO) shows a promising properties that may overcome safety problems caused by carbon anode. However, the low electronic and ionic conductivity are main bottleneck for its application. This research focuses on synthesizing LTO using nano TiO2 synthesized through Sol-gel method. Moreover, the effect of TiO2 crystalline size will be discussed as well. Crystalline size of TiO2 is adjusted by varying calcination temperature at 300oC; 400oC; and 500oC and heated for 6 hours. The crystallite size shown by X-ray diffraction patterns are 7,71 nm ; 15,16 nm ; 23,99 nm, respectively. Furthermore, The obtained TiO2 powder is sintered. The result shows that relatively pure LTO is successfully synthesized using 15,16 nm TiO2. The coin cell are assembled to analyze Li4Ti5O12 anode electrochemical performances.