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Background: The quality of hospital services continues experiencing competition that requires new innovations to nurses. Nurses as providers of nursing care are not only required to achieve physical healing of patients, but also psychological, spiritual, and social aspects, by applying Islamic behavior in every action. In Islamic behavior is influenced by several factors including knowledge of Islamic behavior and attitudes towards Islamic behavior. Objective: This research was conducted to determine the predisposing factors (knowledge and attitudes) that influence the Islamic behavior of nurses. Methods: The research was an analytical survey with a cross-sectional study approach (cross section). The study was conducted in February-August 2019. The population in this study were all inpatient nurses in Aisyiyah Muntilan Hospital, Magelang Regency, with 57 people using purposive sampling, a sample of 49 nurses who were in accordance with the inclusion criteria. The data obtained from the questionnaire measuring instrument with the checklist method. Bivariate analysis using the Spearman test Rank α = 0.05 significance level 95%. Results: The results of the correlation analysis of predisposing factors produced data on the relationship between knowledge about Islamic behavior with Islamic behavior, it was found that the p value = 0,001 and r = 0.537. The relationship between attitudes towards Islamic behavior with Islamic behavior obtained propability p value = 0.092 and r = 0.246. There was a relationship between knowledge about Islamic behavior with Islamic behavior of nurses. There was no relationship between attitudes towards Islamic behavior and Islamic behavior of nurses. So it can be concluded that of the two predisposing factors, which influence nurses Islamic behavior is the knowledge factor about Islamic behavior. Suggestion: From the results of this study, it was necessary to make policies to improve and maintain nurses knowledge and attitudes so that the Islamic behavior of nurses can increase in the provision of nursing care.