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According to WHO 2014, the cancer with the highest morbidity for women is from breast cancer. The treatment chemotheraphy still has its negative points, such as low effectivity, high adverse effects and resistancy to therapy. This has encourage a search for a natural alternative as a therapy or co-chemotherapy for cancer. This study evaluates the anticancer effect and combination of the compound [1,2-epoksi-3(3-(3,4-dimetoksifenil)-4h-1-benzopiran-4on)] propane (EPI) with Doxorubicin in breast cancer culture MCF7. The study is experimental in vitro, the cytotoxicity is analize using (3-(4,5-dimetiltiazol-2-il)-2,5-difeniltetrazolium bromid) (MTT). The IC50 is analized using probit regression calculation using SPSS software. The synergystic effect of EPI and Doxorubicin is analized based on the the value of Combination Index and the data is analized using Compusyn software 1.0. The result of the studies shows that the IC50 for EPI is 50,77 µg/mL and for Doxorubicin is 26,80 µg/mL. CI for EPI and Doxorubicin (EPI-DOX) shows the average at value 0,1-0,7 which means it has synergistic effect. The most synergystic effect at concentration 1/8 IC50 EPI with ½ IC50 Doxorubicin which is 0.00302. In conclusion, the compound EPI shows anticancer effect toward cancer cell MCF7 and strong synergystic effect when combined with Doxorubicin.