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Inhibition of tyrosinase activity from Green Tea (Camellia sinensis (L.) Kuntze) gel
Hanifah Rahmi(1*), Naniek Setiadi Radjab(1), Supandi(1), Tiara Julianti(1)

(1)Faculty of Pharmacy and Science, Universitas Muhammadiyah Prof. DR. HAMKA


Abstract

Green tea (Camellia sinensis (L.) Kuntze) leaf have polyphenol substance that can for inhibitory of tyrosinase enzyme. Tyrosinase enzyme is the one of important component that can be initiated to formation skin of melanin. Green tea leaf was formulated in dosage form of a gel with carbomer 934 as a gelling agent. This study aimed to determine the activity of tyrosinase enzyme with different concentrations of carbomer 934 in green tea leaf extract gel. This research used three carbomer 934 concentration, the first formula was 0.5%, the second was 0.75% and the last formula was 1%. Which every formula was evaluated during 6 weeks involve organoleptic test, homogeneity test, viscosity test, pH test, centrifuge test and freeze-thaw test (during 6 cycles). The optimum formula was evaluated tyrosinase activity used a spectrophotometer. The results showed that the optimum concentration of carbomer 934 was contained on the first formula which has not to phase separation and IC50 value was 49.62 ppm. This research showed that green tea leaf extract gel with carbomer 934 decreased tyrosinase activity.

Keywords: carbomer 934, green tea leaf extract, gel, inhibitory of tyrosinase

Topic: Teknologi Formulasi Sediaan Bahan Alam

Link: https://ifory.id/abstract/xz6At9cJqmXj

Conference: Seminar Nasional Tumbuhan Obat Indonesia ke-57 (POKJANASTOI 2019)

Plain Format | Corresponding Author (Hanifah Rahmi)

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