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Vitamin D is in charge of regulating the amount of calcium in the blood, bones and intestines, besides this vitamin is also useful for increasing the number of cells of phosphate in the bones. Vitamin D is the only vitamin that is produced naturally by the human body, while under the light of the Sun. The purpose of this study is to conduct an analysis of the mineral content and vitamin D on bone flour fish yellowfin tuna. The results of mineral content of flour of the highest fish bones found on calcium and phosphorus (222,54; 107,08 mg/kg), while vitamin D with the best value found in the NaOH treatment (0.119 g/ml), whereas citric acid treatment is (0,173 g/ml) and treatment the control value (0,069 g/ml).