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Application of Response Surface Methodology for Optimizing Antioxidant Extraction from Actinodaphne sesquipedalis leaves
Siti Fatihah Balqis Binti Jamaludin, Siti Salwa binti Abd Gani,Uswatun Hasanah binti Zaidan and Paiman bin Bawon

Halal Product Research Institute, University Putra Malaysia


Abstract

In order to optimize the extraction of antioxidant components from Actinodaphne sesquipedalis leaves, an investigation into the effects of three parameters on DPPH free radical scavenging activity of extracts was studied. Response surface methodology (RSM) was applied to predict optimum conditions for microwave-assisted extraction (MAE) of antioxidative components from A. sesquipedalis leaves. Thus, the objective of this present study was to optimize the extraction condition for the maximum DPPH radical scavenging activity. A Central Composite design was employed to determine the effects of the selected variables, comprising extraction time (20-80 sec), power level (30-60 watt) and ethanol concentration (30-90 %) on the DPPH scavenging activities. Data were analyzed by using Design Expert (version 10.0.1, Stat-Ease, Inc., Minneapolis, MN, USA) statistical analysis software. The optimal extraction conditions for the highest antioxidant capacity were derived at 90 seconds of extraction time, 30 watt of power level and 80 % ethanol concentration that resulted in 92.62 % of scavenging activity. Estimated maximum value at predicted optimum conditions were in good agreement with experimental value. Further research is warranted to investigate the antioxidant activities of A. sesquipedalis leaves extract and their mechanism of action as a basis for future formulation of halal.

Keywords: Actinodaphne sesquipedalis, Response Surface Methodology, DPPH radical scavenging activity, microwave-assisted extraction

Topic: Halal Food, Pharmaceutical, and Cosmetics Manufacturing

Link: https://ifory.id/abstract/ye3F4gVG7vbJ

Conference: International Conference on Islam, Economy, And Halal Industry (ICIEHI 2019)

Plain Format | Corresponding Author (SITI FATIHAH BALQIS JAMALUDIN)

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