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Jember Safety Center (JSC) with Fokus Anak Ibu (FAI) is health information system based on Android to optimize the implementation of pregnancy referral and childbirth women in Jember Regency. All the data of high risk pregnant women will be integrated to this system, so midwife coordinator in Primary Healthcare can access this data with their Android smartphone. This system is one of efforts to decrease maternal and infant mortality rate in Jember Regency. Based on preliminary study, JSC with FAI was not running well. This study aims to analyze the effect of information system usefulness and midwife involvement toward perceived of use of JSC with FAI in Jember Regency. This is an analytic survey research with cross sectional design. The sample of this research is midwife-s coordinator in Primary Healthcare in Jember Regency (45 people). The variables in this study are information system usefulness, midwife involvement and perceived of use of JSC with FAI. Its analysis is carried out through Regressed Multiple Linear. The results showed that information system usefulness had an effect on the perceived of use of JSC with FAI (sig = 0.00) while the involvement of midwife did not affect on the perceived of use of JSC with FAI (sig = 0.244).