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The first 1000 days of life is a golden period for children-s growth and development. There are various efforts made by humans aimed at optimizing the growth and development of their children in this period. In Balinese Hindu community, the way to optimize childrens growth and development in the first 1000 days period is actualized in the form of traditions packed in the Manusa Yajna ceremony. In general, the Manusa Yajna ceremony aims to build human resources that are useful for the community. But in practice, the Manusa Yajna ceremony is interpreted as a sacred ceremony related to the debt of parents to their ancestors who reborn as their children. Manusa Yajna ceremony beyond the sacred context is a form of education dealing with the efforts to optimize the children-s growth and development through nurturing process. This study seeks to explore the meaning behind Manusa Yajna tradition in optimizing childrens growth and development in the first 1000 days period. The analysis will mainly focusing on the process, facilities and infrastructure of the ceremony relating to Tantra, Mantra and Yantra. This research is a qualitative study within the context of Balinese Hindu community.