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Abstract Objective: This study aims to determine estradiol levels in mothers who experience postpartum blues Methods: It was a crossectional study conducted in Pertiwi Maternal and Child Hospital Makassar. The population was all mothers who had normal postpartum births on the third day from January to March 2018 and samples were 31 selected accidentally selected. Postpartum blues data was obtained with the Edinburgh Postnatal Depression Scale (EPDS) questionnaire and secondary data obtained from maternal medical records in the delivery room and nursing staff at the hospital. Estradiol levels examined using the enzyme-linked immunosorbent assay (ELISA) technique. Analysis of the data in this study Mann Whitney and data were performed using frequency and mean. Results: Estradiol levels between mothers who experienced and those who did not experience postpartum blues were different significantly (p=0.024, p<0.05). Mothers with postpartum blues have estradiol levels of 729.92 pg/ml, this level is 293.56 pg/ml lower than those who do not experience the postpartum blues, which is 1023.48 pg/ml. In the group with postpartum blues, the range of postpartum mothers appeared to be at levels of 58.54. Conclusion: Levels of hormone estradiol are lower in mothers who have postpartum blues. It is needed individual attention to postpartum mothers so that those detected with very low estradiol levels can get treatment and prevention as soon as possible.