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Abstract Topic: Agriculture Engineering and Biotechnology

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ALTITUDE OPTIMIZATION USING ULTRASONIC SENSORS OF UNMANNED AERIAL VEHICLE FOR FOLIAR FERTILIZER
Faisal Lutfi Afriansyah, Fendik Eko Purnomo, Nurul Zainal Fanani, Ika Widiastuti,Niyalatul Muna

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Corresponding Author
Faisal Lutfi Afriansyah

Institutions
Department of Information Technology, Politeknik Negeri Jember, Indonesia

Abstract
Flights Unmanned Aerial Vehicle (UAV) really require altitude position. The altitude position of the UAV is used to measure the distance between the UAV when flying with ground. In UAV system are there are two modes of altitude position, absolute height and relative height. In previous research, precision farming uses unmanned aerial vehicles for foliar farming. The research has recommended the best spraying at an altitude of 1.5 meters of plants, but there are problems that UAVs are difficult to maintain a stable height of terrain in agricultural lands by plants. This research will optimize the height of UAV for foliar fertilizer using ultrasonic sensors. UAVs for foliar fertilizer use low flights because they have to be a few meters above the agricultural land. The ultrasonic sensor works well at a distance of 24 cm to 700 cm above the terrain, so it can be used as a sensor in the UAV for foliar fertilizer. The agricultural land used in this research is corn plant in politeknik negeri jember area. The process of this research is by building a UAV without carrying the load of foliar fertilizer. The results of altitude visualization graph show that experiments with ultrasonic sensors produce smoother graphs. Ultrasonic sensor data produces waves with a lot of noise, this can be caused by ultrasonic waves emitted bouncing with the leaves of corn plants.

Keywords
Altitude;Ultrasonic sensor;Unmanned aerial vehicle;Foliar fertilizer

Topic
Agriculture Engineering and Biotechnology

Link: https://ifory.id/abstract/4q2VPDAELYkH


Analysis of Heat Transfer Evaporation Process for Making Ants Sugar Made from Fresh Raw Neera
Rizza Wijaya (a), Budi Hariono (b*), Febryan Kusuma Wisnu (c)

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Corresponding Author
Rizza Wijaya

Institutions
a) Department of Agricultural Technology, Politeknik Negeri Jember
*Corresponding author: budihariono1966[at]gmail.com
b) Department of Agriculture Engineering, University of Lampung

Abstract
Ant sugar is a powdered version of brown sugar and is often referred to as crystal sugar. It is called ant sugar because the shape of this sugar resembles that of ants nesting on the ground. The basic ingredients for making ant sugar are palm juice from coconut and palm trees (palm). In this study, the maximum temperature regulation is 140 oC and the pressure is 2 atm. The materials used in this study were coconut and sugar cane juice produced by farmers in Kokap Wates, Kulon Progo Regency. In the manufacture of ant sugar in the evaporation process the temperature of the material increases with increasing winding temperature and over time. In the process of fresh neera evaporation, the value of heat transfer coefficient ranges between 20-22 (W / m2.oC). This shows that when the initial cooking for this concentration is needed more than 20 Watts to heat the material by 1 oC for an area of ​​1 m2 of material. The value of heat transfer coefficient of turmeric neera beetween 17 - 25 (W / m2.oC).

Keywords
Ant Sugar; Evaporation; Heat Transfer

Topic
Agriculture Engineering and Biotechnology

Link: https://ifory.id/abstract/ejn8Gu7cfYCP


APPLICATION OF AUTOMATIC HATCHING MACHINE TECHNOLOGY IN SOLO DUCK BREEDERS COMMUNITY AT BALET BARU VILLAGE SUKOWONO DISTRICT – JEMBER RESIDENCE
Mochammad Nuruddin, Ujang Suryadi

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Corresponding Author
Mochammad Nuruddin

Institutions
Poiteknik Negeri Jember

Abstract
The purpose of this community service activity is to help the group of duck breeders in the Balet Baru Village, Sukowono, Jember. This livestock group is engaged in the business of raising laying ducks and broiler ducks. Existing breeders in this group have difficulty in obtaining quality ducklings. To overcome this there is one breeder who has tried to produce their own ducklings with good quality. However, for its development there are constraints, namely the limitations of the hatching machine that is owned, other than that its management is still not organized. The target to be achieved in this activity is the breeders are able to produce quality duck seedlings, breeders have one duck-hatching unit, and the formation of better management. The method used is to make a 750-hatch automatic hatching machine, a viewing device, and an automatic incubator.

Keywords
duck, cage, hatchery, livestock

Topic
Agriculture Engineering and Biotechnology

Link: https://ifory.id/abstract/hftWCw9axAzd


APPLICATION OF AUTOMATIC HATCHING MACHINE TECHNOLOGY IN SOLO DUCK CATTLE COMMUNITY AT DESA BALET BARU, KECAMATAN SUKOWONO - KABUPATEN JEMBER
Mochammad Nuruddin, Ujang Suryadi

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Corresponding Author
Mochammad Nuruddin

Institutions
Politeknik Negeri Jember

Abstract
The demand for duck meat and egg in Jember district is quite high. This must be balanced with the production of high ducklings as well. Solo duck cattle community in Balet Baru Village, Sukowono Subdistrict, Jember District is engaged in the business of hatching both laying and broiler duck. In this business traditional hatching machines are still used and have a small capacity. The solution to the problem faced is to implement an automatic hatching machine that has high hatchability and production capacity. The results obtained from this activity are a technology package in the form of an automatic setter machine, a candling box, and an automatic hatcher machine. The setter and hatcher machine is equipped with an automatic control system and has a maximum capacity of 1200 eggs, while for the observation box has a maximum capacity of 49 eggs. In the setter machine is also equipped with a reversing lever to flatten the heat coming from incandescent lamps and heaters. The test results that hatchability for this machine is 82.6% with hatching time for 28 days. The partner of this community service is pleased with the technology applied in the area and hoping that his business is growing much better.

Keywords
Duck, Setter, Candling, Hatching

Topic
Agriculture Engineering and Biotechnology

Link: https://ifory.id/abstract/tmbuYg8Zzq4v


Cavendish Banana Seedling Acclimatization and Enlargement on Some Media Planting Combinations
Ferziana (1), Lisa Erfa (2) , Desi Maulida (3), Yuriansyah (4)

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Corresponding Author
Ferziana ferziana

Institutions
Politeknik Negeri Lampung

Abstract
Growing media is one of the important factors that determine the success of the acclimatization of plantlets from in vitro culture. Several media combinations were tried in this study with the aim of 1) Knowing the effect of media combinations on the success of plantlet acclimatization and seedling growth at the enlargement stage, 2) Getting the best media combination for the successful acclimation and enlargement of banana seedlings, and 3) Seeing the possible use of media husk in acclimatization and enlargement of banana seedlings as a result of tissue culture. The study was conducted at the Lampung State Polytechnic net home from May to August 2019. The research was carried out in a Randomized Block Design. Six media combinations were tried, namely: P1: Topsoil + Husk (1: 1); P2: Top Soil + husk (1: 2); P3: Topsoil + husk charcoal (1: 1); P4: Topsoil + Husk charcoal (1: 2); P5: Top Soil + compost (1: 1); P6: Topsoil + compost (1: 2). In acclimatization (planting until the plantlet is 4 weeks old) each treatment planted 30 plantlets, each with 5 replications, while on the enlargement of the seeds each treatment was tried with 15 replications. Observed variables included: Seed height, leaf width, number of leaves, and wet weight. Data were analyzed by analysis of variance and continued with the BNJ test at a 5% level. The results showed: 1) the combination treatment of media had no effect on the percentage of plantlets that grew, and the growth of the number of seedling leaves at the enlargement stage, but markedly affected the growth of seedling height, leaf width, and seedling wet weight, 2) the best combination of media for success plantlet acclimatization and seedling growth rate (seedling height and wet weight) at the enlargement stage are P5 media (Topsoil + 1: 1 compost), then followed by P6 media (Topsoil + Compost 1: 2), and 3). Use of husk media for additional acclimatization media, it can still be used with the success rate of plantlets still reaching 70%, but it is not good to be used at the stage of seedling enlargement until it is ready for planting into the field.

Keywords
acclimatization, cavendish banana, seedling

Topic
Agriculture Engineering and Biotechnology

Link: https://ifory.id/abstract/DzrFnj9ce2EU


DESIGN AND DEVELOPMENT OF YOUNG COCONUT SKIN MACHINERY TO SUPPORT FRESH MARKETING OF FRESH YOUNG COCONUTS IN THE STATE AND EXPORT
Anang Supriadi Saleh, Supriyono, Yana Suryana, Agus Santoso, Ansarullah,Gufron Ainur Refaldi

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Corresponding Author
Anang Supriadi Saleh

Institutions
State Polytechnic of Jember

Abstract
The research aims to make the design of a young coconut leather peeler machine to support the marketing of fresh young coconuts domestically and exports. This research is an appropriate technology to help people in young coconut marketing activities sold in the form of fresh or export. This prototype was made simple to be portable and reasonably priced, but the capacity was great and the quality of the results was better. The design approach for designing the machine is using methods compiled by Gerhardt Pahl and Wolfgang Beitz, so that the design approach is based on a functional design approach and Structural design approaches. The design stages of this machine include designing and manufacturing of tools, laboratory tests, and field tests. The result of this research is the product of young coconut leather peeling machine to support the marketing of fresh young coconut in the country and export, which is ergonomic, affordable, and ready to be applied, has a working capacity of 80 pieces/hour, the results are more neat and symmetrical compared to manual way.

Keywords
design, machine, peeler, young coconut skin, efficient

Topic
Agriculture Engineering and Biotechnology

Link: https://ifory.id/abstract/BERhFvDK4Gea


Development of Plant Monitoring Systems Based on Multi-Camera Image Processing Techniques on Hydroponic System
Rizza Wijaya (1), Budi Hariono (1*), Tri Wahyu Saputra (2)

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Corresponding Author
Rizza Wijaya

Institutions
1) Department of Agricultural Technology, Politeknik Negeri Jember
2) Department of Agrotechnology, University of Jember
*Corresponding author : budihariono1966[at]gmail.com

Abstract
The research objective is to develop a monitoring system for the growth of red spinach plants based on image processing techniques from images captured using multiple cameras. The plant used is red spinach (Amaranthus gangeticus L.). Three cameras are installed in the top, side and front position of the plants in the photo box with lighting every 2 days up to 39 days. Model development uses a sample of 236 plants divided into 178 plants for model development and 58 plants for model testing every two days. The model is tested with the coefficient of determination (R2) to measure how much the independent variables ability to explain the dependent variable. The network architecture consists of three input neurons, first hidden layer with five neurons, second hidden layer with five neurons, and output layers with one neuron. The function of ANN with value of the learning level is 0.001. The activation function to predict fresh weight and leaf area of plants is tansig-logsig-tansig and tansig-tansig-logsig. ANN model can predict fresh plant weight with MSE value of 0.02385 and RMSE of 0.154, while for leaf area MSE value of 0.26428 and RMSE of 0.514.

Keywords
Ann; Hydroponic; Image processing; Red Spinach

Topic
Agriculture Engineering and Biotechnology

Link: https://ifory.id/abstract/KGdWc4bRvzu3


EBERHARD-RUSSEL STABILITY TEST FOR TEN SEGREGATION LINES IN FIVE SOYBEAN CENTRALS OF EAST JAVA
Mohammad Setyo Poerwoko, Nurul Sjamsijah

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Corresponding Author
Mohammad Setyo Poerwoko

Institutions
Faculty of Agriculture Jember university
Department of Agriculture Production

Abstract
Soybean is an important plant because of its role as a source of vegetable protein and as an industrial raw material. National soybean production is still low so that every year imports need to be large enough to meet national needs. National soybean production in 2014 amounted to 892.60 thousand tons while soybean production in Java reached 566.30 thousand tons whereas 329,461 tons/year came from East Java or 42.43% with productivity ranging from 1.3-1.5 tons/hectare. National soybean productivity has only reached 1.2 tons per hectare to date. National soybean needs to reach 2.4 million tons each year while farmers- production is about 850 thousand tons or about 65% of the needs. As the result, the remaining 1.55 million tons is imported from America. Candidates for new superior varieties, which are G-1, G-2, G-3, G-4, G-5, UNEJ-1 and UNEJ-2 have a potential yield of 2.5 tons/hectare and are of early maturity (pod mature age at 73-76 days) and have resistance to the main disease of soybeans, leaf rust. It is expected that these lines can have a stake in achieving sustainable food security. The study was conducted in April to November 2018 in five soybean production centrals in East Java, namely the experimental plant Research Institute for Beans and Tubers (BALITKABI) in Genteng-Banyuwangi, Muneng-Probolinggo, Ngale-Ngawi, Kendalpayak-Malang, as well as Experimental Garden of Jember State Polytechnic. The experimental design used for each location was randomized block design with ten treatments (genotypes) and three replications. The size of each plot (unit) of the experiment was 2.8 m x 4.5 m or around 12.6 m2 (used by the National Soybean Consortium). The distance between rows was 40 cm and inside the rows was 15 cm. Each hole was filled with three seeds, and after growing, two healthy plants were remained. To find out the interaction between the genotypes and the environment, analysis with a randomized group design was used. Next, the stability was analysis by the Eberhart and Russel method. The result of stability analysis showed that genotypes G-1, G-2, G-3, G-4, G-5, and UNEJ-1 were stabile in five locations, whereas UNEJ-2, Malabar and Ringgit turned out to be unstable.

Keywords
Eberhard-Russel Stability Test Results for Ten Advanced Segregation Strains

Topic
Agriculture Engineering and Biotechnology

Link: https://ifory.id/abstract/92RzJ3Q7bZmM


Endogenous Hormones at The Beginning of Leaf Growth after Vernalization of Garlic Bulb (Allium sativum L.) in Indonesian Local Varieties
Edi Siswadi1, Sekar Utami Putri1, Refa Firgiyanto1, Nuhak Nufitasari1, M. Zayin Sukri1, Cholyubi Yusuf2

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Corresponding Author
Edi Siswadi

Institutions
1 Department of Agricultural Production, State Polytechnic of Jember, Mastrip Street 164 Jember, 68121, Indonesia
2 Department of Agribusiness Management, State Polytechnic of Jember, Mastrip Street 164 Jember, 68121, Indonesia

Abstract
Induction of garlic flowering can be done by vernalizing the seed tubers to trigger flowering. This study aims to determine the hormone status of IAA, GA3, at the time of initial growth of leaf tissue, after going through vernalization. Bulbs of Indonesian local varieties of Lumbu Kuning and Tawangmanu varieties were vernalized for 4 weeks, 8 weeks, and without vernalization treatment as a control. The contents of IAA and GA hormones in the leaf tissue of garlic plants aged 2 weeks were analyzed using method high performance liquid chromatography (HPLC). The results showed that the Lumbu Hijau variety with 4 weeks vernalization contained IAA of 1615,595 ng / g; GA3 is 7.297 ng / g. At 8 weeks vernalization the IAA content is 2169,475 ng / g; GA3 of 9,757 ng / g; In the Tawangmanu variety with 4 weeks vernalization containing IAA of 1459,168 ng / g; GA3 is 6.617 µg / g. Whereas for 8 weeks vernalization of IAA content of 2007,195 ng / g; GA3 is 8.315 ng / g.

Keywords
Garlic, Vernalization, IAA dan GA

Topic
Agriculture Engineering and Biotechnology

Link: https://ifory.id/abstract/Fw6c3kRhQDAp


Finite Impulse Response type multilayer perceptron artificial neural network model for bacteria growth modelling inhibited by lemon basil waste extract
T Budiati*1, W Suryaningsih1, T R Biyanto2, N P Pangestika3, M T Pangestu3, F Saputra3, A Hidayat3, A Widyawati3, F N Firdaus4 and D V Sabilla3

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Corresponding Author
Titik Budiati

Institutions
1 Food Engineering Department, Politeknik Negeri Jember, Mastrip PO Box 164
Jember 68101 East Java, Indonesia
2 Department of Engineering Physics, Institut Teknologi Sepuluh Nopember Surabaya, Surabaya 60111 East Java, Indonesia
3 Food Industrial Technology Department, Politeknik Negeri Jember, Mastrip PO
Box 164 Jember 68101 East Java, Indonesia

Abstract
The tools to predict the growth of bacteria over the time is essential to maintain the process stability in bio processes. Currently, no such tools are used in industries or laboratories. In this paper, an approach based on Finite Impulse Response (FIR) type multi layer perceptron aritficial neural network model is proposed. The neural network model was developed using data collected from laboratory work. As amount of 75% growth of bacteria (S. Aureus, B. Cereus and S. Typhimurium) which is inhibited by lemon basil waste extract, over the time data are used to train Artificial Neural Network (ANN), and the rest of the data are used to validate the model. ANN has been model the growth of S. Aureus, B. Cereus and S. Typhimurium which is inhibited by lemon basil waste extract over the time. It was observed that the Mean Square Error (MSE) during training is 0.087 and validation is 0.147. It proving that the modeling approach employed in this research is suitable to capture the nonlinear characteristics of the bacteria growth.

Keywords
bacteria growth; prediction; ANN; MLP; FIR

Topic
Agriculture Engineering and Biotechnology

Link: https://ifory.id/abstract/9gweYtTn3JkE


Flowering Induction of Phalaenopsis Orchids with a Combination of BAP and Temperature Concentrations
Lisa Erfa(1) , Desi Maulida (2), Ferziana (3), Rizka Novi Sesanti (4), Yuriansyah (5)

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Corresponding Author
Lisa Erfa

Institutions
Politeknik Negeri Lampung
Jl. Soekarno Hatta No 10 Rajabasa Bandar Lampung

Abstract
The length of time needed to produce orchids both in the laboratory and to produce mature flowering plants is an obstacle in the development of orchids. Generally, it takes a long time to get the first flowers from plants that are propagated. This study aims: 1) to determine whether BAP growth regulators can stimulate flowering and growth of Phalaenopsis orchid plants, 2) whether the influence of BAP zpt depends on air temperature, 3) get the best concentration to stimulate flowering and plant growth. The research was carried out at the Lampung State Polytechnic net house from June to August 2019. The research was a factorial experiment in a completely randomized design. As factor I is BAP concentration (0, 50,100,150, 200 mg / l) and factor II is night air temperature (26 / 180C and 32/260 C). There were 10 treatment combinations were tried, with 5 replications. Observed variables include data analyzed by analysis of variance, then followed by a 5% LSD test. The results showed: From the results of this study the following conclusions can be drawn: 1) BAP concentrations can significantly stimulate flowering (spike emergence) & height growth of Phalaenopsis orchid seedlings, and significantly affect leaf length and number of leaves; 2) The effect of BAP concentration interacts/depends on air temperature; 3) The number of spikes per plant is markedly better / more formed at normal temperatures. At normal temperatures, a good concentration of BAP in producing spikes is 100 mg / l, whereas at low temperatures the amount of spike is most when given the addition of BAP 50 mg / l; and 4) At normal temperature the BAP concentration that is good for seedling growth (seedling height, leaf length, leaf width, and number of leaves) is 100 mg / l, whereas at low temperatures BAP concentration is good for seedling growth (seedling height, leaf width, and the number of leaves) is 50 mg / l.

Keywords
BAP, temperature, Phalaeonopsis orchid

Topic
Agriculture Engineering and Biotechnology

Link: https://ifory.id/abstract/wxLNcY8qAV9T


Heritability and Genetic Gain of the Agronomic Characters of Crosses between Local Black Rice and White Rice in F2 Generation
Dwi Rahmawati, Hari Prasetyo, and Putri Santika

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Corresponding Author
DWI RAHMAWATI

Institutions
Departement of Agricultural Production, State Polytechnic of Jember

Abstract
Black rice is a local variety that has advantages and disadvantages. This rice has a high anthocyanin content. However, it has a long life span, high plant habitus, and low yield potential. Productivity improvement of black rice can be done through crossing. The purpose of crossing between two having certain superiority is to assemble superior cultivars and proceed with selection. The effectiveness and efficiency of selection is determined, among others, by the value of heritability and genetic gain. This study aims to determine the genetic gain and the estimated value of heritability of the agronomic characters of F2 generation resulting from crosses between local black rice with several superior varieties of white rice. The research was carried out in the Jember State Polytechnic seed production field, from May to November 2019. This study was conducted using experimental design without replication. The estimated genetic parameters are broad-sense heritability and the genetic gain. The seeds used were F2 generation from the crossing of Arum, BSM, Sertani 9, Cidenuk and Cimelati varieties with local black rice. The observed characters include qualitative and quantitative characters. The expected output target of this research is to obtain one or more lines of hope from crossing results that can be developed.

Keywords
heritability, agronomy character, and paddy rice

Topic
Agriculture Engineering and Biotechnology

Link: https://ifory.id/abstract/BMP839p24b7d


IMPROVEMENT OF VANILLA CULTURE (Vanilla planifolia) THROUGH MICROPROPAGATION TECHNIQUE WITH MODIFICATION OF SITOKININ
Dyah Nuning Erawati (a*), Irma Wardati (b) and Siti Humaida (c)

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Corresponding Author
Dyah Nuning Erawati

Institutions
a, b, c. Lecturer at the Department of Agricultural Production, Jember State Polytechnic, Jl.Mastrip PO Box 164 Jember - Tel. + 62-0331-333532, Fax. + 62-0331-333531
1 dyah_nuning_e[at]polije.ac.id 2irmawardati[at]gmail.com

Abstract
The constraints of vanilla development in conventional vegetative propagation are the presence of Fusarium wilt disease and the limitations of the parent plant as cuttings so that vanilla is developed through micro propagation techniques. The ability of the explants to regenerate and differentiate to form buds in vitro is excessive need to be controlled through cytokine regulation in an effort to get the same vanilla seedlings as parent, healthy and uniform. The objectives of this research are: 1) developing vanilla seedlings through multiplication of shoots by modifying BAP and Kinetin in vitro and 2) obtaining vanilla seeds through uniform, healthy and not attacked by plant disease pests through micro propagation techniques. The study was conducted in the Jember State Polytechnic Network Culture laboratory using a factorial Complete Randomized Design (CRD). Factor 1 is BAP concentration that is 0, 1, 2, 3, 4 ppm and factor 2 is Kinetin concentration which is 0, 1, 2 ppm. Further data analysis used Duncans average difference test of 5% or 1%. The parameters observed in this study were: 1) the budding speed, 2) the number of shoots, 3) the length of shoots, 4) the wet weight of shoots and 5) the development of explants. Achievement of the expected results in this study has a value of Technological Adequacy Level (TKT) 5 which is a picture of validation of cytokine modifications that effectively produce multiplication of vanilla shoots so that the same vanilla seedlings are obtained as the parent, uniform, healthy.

Keywords
BAP, kinetin, tissue culture, vanilla

Topic
Agriculture Engineering and Biotechnology

Link: https://ifory.id/abstract/CPU4X7y3vJAq


In Situ Mariculture for Cultivating Bioactive Compound Producing Sponges
Walter Balansa, Stevy Imelda Wodi, Frans G. Ijong, Deidy Azhari, Aprelia Tomasoa, Frets J. Rieuwpassa

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Corresponding Author
Walter Balansa

Institutions
Politeknik Negeri Nusa Utara

Abstract
Marine sponges represent an important source of bioactive molecules with antibiotic, anticancer, antimalarial, antiaging and anti-HIV activities, to name but a few. However the scarcity of bioactive compounds found in sponges remains a bottleneck in marine drug discovery program, hampering research and development as well as posing a serious threat to marine environment due to an excessive harvest of marine sponges. To tackle the supply and environmental issues, we focus our study on in situ cultivation of 15 sponges from Enepahembang Tahuna, North Sulawesi, Indonesia. The sponges were individually placed on a concrete substrate installed on a PVC (Polyvinyl chloride) module and monitored periodically for 12 months. Cultivation efficiency was evaluated by growth rate, survival rate and antibiotic activity particularly for antimicrobially active sponges. While the total survival rate was 67%, growth rate varied between 18% and 97% with Clathria reinwardti, Acanthostrongylophora ingens, Asteropus sarassinorum showing the highest growth rate. Although they were inactive against our test bacteria, C.renewardti has been known for its ability to biocumulate heavy metal, A. Sarassinorum as the source of the glycosides with antitumor, antifungal and/or antiprotozoan activities dan A. ingens as the antimalarial manzamine producer. In addition, antimicrobial evaluation revealed a striking similarity of antimicrobial profiles between the cultivated and wild sponges. Together, they suggest the importance of in situ mariculture for supplying bioactive compounds and for implementing enviromentally friendly drug discovery program.

Keywords
Antibiotics, sponges, bioactive compounds, growth rate, survival rate, in situ mariculture

Topic
Agriculture Engineering and Biotechnology

Link: https://ifory.id/abstract/Qu7UCBRAvPZw


In Vitro Regeneration of Stevia Rebaudiana Bertoni from internode and leaf explants using different concentrations of BAP (6-Benzyl Amino Purine)
Sepdian Luri Asmono (a*), Djenal (a), Rahmawati (a)

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Corresponding Author
Sepdian Luri Asmono

Institutions
a) Plant Production Departmen, Politeknik Negeri Jember, Jalan Mastrip, PO BOX 164 Jember, East Java, Indonesia
*sepdian[at]polije.ac.id

Abstract
The aim of this study was to determine the response of the growth of internode explants and leaves of Stevia (Stevia Rebaudiana Bertoni), which were cultured at several BAP concentrations. This study was designed using a non Factorial Complete Randomized Design that is by testing the internode and leaf explants at 3 different BAP concentration levels of 2, 3 and 4 ppm and will be repeated 10 times. The parameters observed related to callus growth include: emergence, type, color and percentage of callus formation. In addition, observations were also made on the parameters of bud growth including the appearance of shoots, number and length of shoots. The results of this study indicate that 100% of leaf and internode explants are able to form callus. The color of the callus explants on average leaves are yellowish green while the internode explants are yellowish white and all have compact textures. In shoot growth parameters, only internode explants were able to grow shoots while leaf explants were not. The emergence of shoots (3 DAP), number of shoots (8.70), and shoot length (2.81cm) were produced by MS media with the addition of 3ppm BAP.

Keywords
Stevia, In Vitro, BAP (6-Benzyl Amino Pourine), Explant, Multiplication

Topic
Agriculture Engineering and Biotechnology

Link: https://ifory.id/abstract/98Lg7Fxvutc4


Increasing of Aceh-s Patchouli Production with Technology of Bio-fertilizer Local Specific Mycorrhizal Strains in Entisols
Syafruddin1, Syakur2, Erida Nurahmi1, Erita Hayati1, Nurhayati1, Elly Susanti2

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Corresponding Author
Syafruddin Syafruddin

Institutions
1) Department of Agrotechnology, Universitas Syiah Kuala
2) Department of Soil Science, Universitas Syiah Kuala
3) Department of Agribusiness, Universitas Syiah Kuala

Corresponding author: syafruddin[at]unsyiah.ac.id

Abstract
Aceh-s patchouli is one of the superior local commodities, and its growth and yield could be improved in various means. One way to increase the patchouli production can be done by using bio-fertilizer mycorrhizal local strain. The purpose of this research was to study effect of bio-fertilizer mycorrhizal local specific strain on the growth and yield of patchouli in Entisols. The research was arranged by a non-factorial randomized block design with three replications. The investigated factor was the bio-fertilizer mycorrhizal from local specific strain with four levels, i.e. M0 (without mycorrhizae), M1 (Glomus mosseae), M2 (Gigaspora sp) and M3 (Mixing between Glomus mosseae and Gigaspora sp). The variety of patchouli used in this study was the Tapak Tuan variety. The observed parameters were plant height and stem diameter of patchouli at 45, 60 days after planting; additionally the parameter of patchouli yields consist of number of leaves, number of branches and weight of leaves were observed at 90 days after planting, and root colonization by mycorrhizae. The results showed that mixed mycorrhizal strains between Glomus mosseae + Gigaspora sp contributed to the best marks for increasing growth and yield of patchouli in Entisols. The similar trend result was also found in the root colonization by mycorrhizae. The strain of mycorrhizal of Gigaspora sp application was not effective for increasing the growth and yield of Aceh-s patchouli in Entisols.

Keywords
Aceh-s patchouli, bio-fertilizer, local mycorrhizal strain, yield, Entisols

Topic
Agriculture Engineering and Biotechnology

Link: https://ifory.id/abstract/A7jtm9K4xzLB


PHYSICAL AND CHEMICAL CHARACTERISTICS OF OYSTER MUSHROOMS FLOUR (Pleurotus ostreatus) USING ROTARY VACUUM DRYER TYPE BATCH
Siti Djamila (a*), Amal Bahariawan (a), Iswahyono (a)

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Corresponding Author
Siti Djamila

Institutions
a) Departement of Agricultural Engineering
Politeknik Negeri Jember
jalan Mastrip 164
a*) umi.djamilasy[at]gmail.com

Abstract
Harvested oyster mushrooms with high moisture content are easily damaged by enzyme and microorganism. Drying the mushroom into flour using sun drying or artificial dryer can extend the storage time. Rotary vacuum dryer, an artificial dryer, works on vacuum conditions with automatic material reversal technology. The vacuum conditions can reduce boiling point and minimize the nutrition damage. The purpose of this study is to compare the physical and chemical characteristics of oyster mushroom flour produced by rotary vacuum dryer and sun drying. Rotary vacuum dryer works at 60 o C for 8 hours while sun drying requires 6 hours for 4 days. Next, 80 mesh hammer mill is used to grind the dried oyster mushroom. The process includes physical and chemical test analysis with the statistical results followed by t test to understand the two treatments average differences. The results show that all chemical characteristics: moisture content, protein, ash, dietary fiber, produced by rotary vacuum dryer is not significantly different from sun drying. The physical characteristic: color (lightness) and the water activity are not significantly different but the water holding capacity and bulk density are significantly different.

Keywords
oyster mushroom , rotary vaccum dryer , physical and chemical characteristics

Topic
Agriculture Engineering and Biotechnology

Link: https://ifory.id/abstract/Pu8Vy2wWZDn6


Response of the Vanda Planlet (Vanda sanderiana) to the Addition of Guano and Mycorrhizal Fertilizers in the Acclimatization Stadia
Kasutjianingati (a*), Refa Firgiyanto (a), Alfonsina Ella Warisu (a)

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Corresponding Author
KASUTJIANINGATI KASUTJIANINGATI

Institutions
a) Departemen of Agricultural Production, State Polytechnic of Jember, Jl. Mastrip, Jember, Indonesia
*kasutjiningati[at]gmail.com

Abstract
Acclimatization is a critical point for the success of tissue culture propagation. In this phase, in addition to maintaining the condition of the growing environment, accuracy of media composition is required. This study aims to determine the growth response of the Vanda orchid plantlet (Vanda sanderiana) acclimatization phase to the dose of guano and mycorrhizal fertilizers. The design used in this study was a factorial Randomized Block Design (RBD) with 2 factors. The first factor is the dose of guano fertilizer (without guano, 4g/plant and 8 g/plant), the second factor is mycorrhizal dose (without mycorrhizal, 10 g/plant and 20 g/plant). Growth observation variables include number of leaves, leaf length, leaf width and life percentage. The experimental results were analyzed using Analysis of Variace and followed by 1% BNJ test. The results showed that the interaction of the two treatment factors did not significantly affect all parameters. Conclusions guano and mycorrhizal fertilizers are not needed in the acclimatization phase of the Vanda orchid plantlet.

Keywords
Vanda Planlet, Guano, mycorrhizae, acclimatization

Topic
Agriculture Engineering and Biotechnology

Link: https://ifory.id/abstract/9cdx8BKHbkGy


Serine protease from Artocarpus altilis (breadfruit) latex
Siti-Balqis Zulfigar, Rosma Ahmad

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Corresponding Author
Siti Balqis Zulfigar

Institutions
Bioprocess Technology Division, School of Industrial Technology, Universiti Sains
Malaysia, 11800 USM, Penang, Malaysia

Abstract
Breadfruit is recognized as a plant with the potential to be cultivated as an alternative staple food due to its high starch content and envisioned as a solution to mitigate hunger. The plant bears abundant fruits throughout the year with its productivity is comparable to other staple crops and thrives well in countries where the poor population is high. Breadfruit however, exudes copious amount of latex upon harvesting and the stained epicarp often perceived as low in quality. Standardized methods of harvesting in plantations include a stage of latex draining by inverting the fruit for several hours. The latex will be drained to the ground and considered as an agricultural waste with no current commercial application. Despite being considered as a nuisance, plant latex is rich in proteases functioned as a form of protection against pathogenic attacks. In the effort to identify its potential, the breadfruit latex protease was purified to an apparent homogeneity and its optimal conditions were determined. The stability of the protease was investigated and thermodynamic parameters were estimated in this research. Therefore, these analyses had revealed that breadfruit latex consists of protease with highly stable properties, potentially developed as an alternative commercial protease.

Keywords
Artocarpus altilis, latex, serine protease

Topic
Agriculture Engineering and Biotechnology

Link: https://ifory.id/abstract/wYKAuBn4fhz8


SUSTAINABLE MARINE ECOTOURISM DEVELOPMENT STRATEGY IN MERAUKE REGENCY
R Abdoel Djamali1, Dhanang Eka Putra2

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Corresponding Author
R ABDOEL DJAMALI

Institutions
1, 2Agribusiness Management Departement, Politeknik Negeri Jember, Jember 68101
East Java, Indonesia

Abstract
Merauke Regency is a plain area which is between 0-60 meters above sea level and the south and west are bordered by the Arafuru Sea. Flat areas become centers of population that use land for cultivation activities and become a concentration of settlements. While the coastline of the Merauke Regency is potentially developed into a maritime-based tourist destination, which is often called Marine Ecotourism. The research objectives are: designing a strategy for developing marine ecotourism in Merauke Regency. The research method used is the purposive sampling research design, expert system research methods, and analysis techniques with the Analystical Hierarchy Process (AHP). The conclusions in this study are: (a) Priority goals is: the preservation of sustainable coastal resources especially mangroves, growing a new center of economic growth, preparing the location of new marine tourism destinations, increasing the contribution to local revenue, ( b) The priority of the main actors is: private, private together with the government, and government, (c) The development faculties are: structuring of coastal areas and exploitation of sand and mangroves, structuring of acceptance to marine tourism areas, integrated socio-economic engineering and technology, developing fishery-based agro-industry SMEs, and promoting and attracting investors for the development of marine tourism.

Keywords
Strategy, Marine Ecotourism, Merauke

Topic
Agriculture Engineering and Biotechnology

Link: https://ifory.id/abstract/Jd9naWCP2bFg


The Effect of Varieties on Growth and Yield of Chili (Capsicum annum L.) in Andisol Soil Aceh Besar
Syafruddin1, Syamsuddin1, Syakur2, Jumini1, Ainun Marliah1, Manfarizah2, Hasanuddin1

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Corresponding Author
Syafruddin Syafruddin

Institutions
1) Department of Agrotechnology, Universitas Syiah Kuala
2) Department of Soil Science, Universitas Syiah Kuala
Corresponding author: syafruddin[at]unsyiah.ac.id

Abstract
This research aims to determine the effect of varieties on the growth and yield of chili plants on the Andisol soil. This research was carried out at Lon Baroh, Lembah Seulawah District Aceh Besar, greenhouse and laboratory of plant physiology, Faculty of Agriculture of Syiah Kuala University, Darussalam, Banda Aceh from November to March 2018. The analysis of data used in this study was Randomized Block Design – non Factorial with 3 replications. There are 2 series of this research, Serie A and Series B. Serie A is used to collect data on growth and yield of chili plants and Serie B is used to collect data on mycorrhizal infection. The factors observed in this research were varieties. Varieties used are Lado F1 and Perintis.The result of this research showed varieties of chili plants highly significant take effect on plant height 15, 30 and 45 DAP, stem diameter 30 and 40 DAP, productive branch numbers, plant fresh weight, plant dry weight, fruit weight, fruit length, fruit number and yield potential and significantly affect on stem diameter 45 DAP, root fresh weight, and root dry weight. The best growth and yield of chili plants is found in Lado F1 varieties.

Keywords
Chili, Andisol soil, varieties, growth, yield

Topic
Agriculture Engineering and Biotechnology

Link: https://ifory.id/abstract/bk3PpBuG69DA


The Growth of Vanda Seedling (Vanda sanderiana) with Some Types of Plant Media Acclimatization and Concentration of Humic Acid
Kasutjianingati (a*), Refa Firgiyanto (a), Lusi Virnawati (a)

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Corresponding Author
KASUTJIANINGATI KASUTJIANINGATI

Institutions
Departemen of Agricultural Production, State Polytechnic of Jember, Jl. Mastrip, Jember, Indonesia

Abstract
Vanda is one type of orchid that has the potential to be developed because it has a variety of shapes and colors. The aim of this research is to find out the right combination of acclimation planting media and humic acid for the growth of hybrid Vanda orchids (Vanda sanderiana). Experiments using factorial Randomized Block Design (RCBD). The first factor is the planting media (zeolite, fern roots, and bagasse) and the second factor is the concentration of humic acid (0 mg / L, 50 mg / L, and 100 mg / L). The results showed that the interaction between the treatment types of planting media and some levels of humic acid concentration showed no significant effect on the percentage of seedling life and on plant growth parameters (height and number of leaves), but significantly different in plant height on the planting media factor. The best growing media is zeolite stone

Keywords
Humic acid, media, zeolite, acclimatization, vanda

Topic
Agriculture Engineering and Biotechnology

Link: https://ifory.id/abstract/6YkAFevVZbBK


The use of 2,4-D and thidiazuron for induction of kopyor coconut callus
Desi Maulida (a), Lisa Erfa (b), Hilman Hidayat (c), Rizka Novi Sesanti (d)

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Corresponding Author
desi maulida

Institutions
a) Horticulture, Politeknik Negeri Lampung
Jl. Soekarno Hatta No 10 Rajabasa Bandar Lampung

Abstract
The low production of kopyor coconuts is caused by kopyor-producing coconut plants that only rely on kopyor-bearing coconut trees that have heterozygot genetic constitution (Kk) and are grown from normal phenotypic coconut seeds. Kopyor fruit cannot be grown naturally. One alternative to get true to type kopyor seeds is by embryo culture. Development of reliable clone propagation such as somatic embryogenesis will quickly provide embryos for kopyor. From one kopyor em-bryo that is produced will get many somatic embryos which can then become kopyor coconut seeds. The aim of this research is getting an effective method to produce true to type kopyor seeds and knowing the influence of 2,4-D with or withaout TDZ in inducing callus to produce somatic embryos. This research was carried out in the Lampung State Polytechnic laboratory using a factorial. Factor 1 is 2,4-D concentration 0, 2,5, 5, 7.5, and 10 mg/l with or without the addition of 0.5 mg/l. Further data analysis using the 5% LSD test. The parameters observed in this study were: 1) Percentage of explants callus 2) Callus diameter, 3) Callus weight. The results showed that Increasing concentrations of 2,4-D and 7.5 mg/l can increase the percentage of embryogenic callus, callus diameter, and weighing callus. The addition of TDZ concentration of 0.5 to the medium 2,4-D resulted in a decrease in the percentage of embryogenic callus, callus diameter is narrower and lower weight on embryonic callus kopyor coconut. The concentration of 2,4-D best to induce callus on embryo kopyor is 7.5 mg/l.

Keywords
Kopyor, 2,4-D, TDZ

Topic
Agriculture Engineering and Biotechnology

Link: https://ifory.id/abstract/d7NgwThqUeu4


The Utilization of Organic Materials, Zeolites and Sugarcane Compost in Improving Water Retention, Cation Exchange Capacity and Growth of Chili Plants in Sand Land
M. Zayin. Sukri1, Sugiyarto1, Refa Firgiyanto1

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Corresponding Author
muhammad zayin Sukri

Institutions
1Departemen of Agricultural Production, State Polytechnic of Jember, Jl. Mastrip, Jember, Indonesia

Abstract
Land is one of the basic needs in agricultural cultivation. Considering the vast land area of the coast, there needs to be an accurate thought in utilizing the land area of the coast for an alternative farming business with all the consequences so that its limitations can be overcome. Puger District is one of the districts that has been central to the development of horticultural crops since 15 years ago by utilizing sand in the coastal areas. The problem of chili cultivation in puger is still not optimal because of some problems such as plant growth that is not optimal because it is not supported by adequate nutrition and sufficient water. Therefore, there is a need for technological applications in increasing soil fertility in sand fields in order to optimize agricultural production of horticultural commodities, including by providing organic material, zeolite sand and sugarcane stem compost. The purpose of this research is to get the best dose of organic fertilizer, zeolite and sugarcane compost in improving water retention, and cation exchange capacity and chilli growth in Puger District sand and to get the best interaction dose between organic fertilizer, zeolite and sugarcane compost. This research activity will be carried out from May to November 2018. The research was conducted at the Green House Laboratory of Plants and Bioscience - POLIJE, Jember East Java at an altitude of 90 m above sea level. The design that will be used is the design of RAK with 3 factors. The first factor is the dose of organic fertilizer with a level of 20 tons ha-1 and 40 tons ha-1, the second factor is the dose of zeolite with a level of 20 tons ha-1 and 40 tons ha-1 and the third factor is the compost dose of sugarcane stems with a level of 20 tons ha-1 and 40 tons ha-1. The total combination of treatments amounted to 8 and repeated 3 times with each unit consisting of 4 plant pots so that a total of 96 unit plant pots. Analysis of the experimental results includes physical analysis of the soil such as specific gravity, water retention, freezing content, field capacity, and porosity.

Keywords
Biology, Physical, Chemistry, Fertile, Sand

Topic
Agriculture Engineering and Biotechnology

Link: https://ifory.id/abstract/pHWXMgLTZx4e


YIELD ENHANCEMENT OF SOYBEAN (Glycine max L. Merill) IN GENOTYPES ‘POLIJE-4- AND ‘POLIJE-5- THROUGH BACKCROSS WITH LARGE SEED DONOR PARENT
Nurul Sjamsijah, Sri Rahayu, Suharjono, Eva Rosdiana and Putri Santika

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Corresponding Author
Nurul Sjamsijah

Institutions
Politeknik Negeri Jember

Abstract
Soybean is one of important crops in Indonesia because of its role as a source of vegetable protein and it is used as an industrial raw material. Nonetheless, national soybean production is low and cannot keep up with the need. In 2018 alone national production was around 980 thousand tons, whereas the national need for soybeans is around 3 million tons / year. Hence, large amount of import is required to meet national needs every year. This study aims to assemble new high yielding soybean varieties, which have high yield (≥3 ton/ha), early maturity (harvest age ≤76 days), large seed size (>15 g) and have moderate resistance to major soybean disease, the leaf rust, caused by pathogen Phakopsora pachyrhizi. In the previous research, soybean genotypes which fit to the criteria above have been obtained, namely ‘Polije-4- and ‘Polije-5-. However, these genotypes have a major drawback which is the small size of the seed (around 13 g/100 seeds). This drawback can be repaired by using the recurrent backcross method with ‘Ryokko- edamame soybean as the donor parent, which has a distinctly large seed size around 35 g/100 seeds. In the current research, this backcross method between ‘Polije-4- and ‘Ryokko-, as well as ‘Polije-5- and ‘Ryokko- was conducted and resulted in the increase of yield (>20 g/plant) and seed size (>15 g/100 seeds), but still maintaining the early harvest age (≤ 76 days).

Keywords
Recurrent Backcross, Edamame Soybean, Seed Size

Topic
Agriculture Engineering and Biotechnology

Link: https://ifory.id/abstract/eNGjUgPzXru8


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