Indonesia Conference Directory

Corresponding Author
Suyana Suyana

Institutions
Department of Medical Laboratory Technology Poltekkes Kemenkes Yogyakarta, Indonesia

Abstract
Bacterial growth media have a diversity of nutrients. Culture media is very important for isolation, testing the physical and biochemical properties of bacteria and for diagnosing a disease. The present study aimed to examine the effectiveness of soybean seed sprouts, green beans, red beans, and peanuts, whether it has the potential to grow the breed Gram positive and Gram-negative bacteria. The research method, nut bean seeds were washed, germinated, dried in an oven at 40oC, blended and sieved in term to obtain flour. Flour was extracted to obtain extract solution plus agar powder and broth was made in agar media in petri dishes as well as formulas and compositions on Nutrient Agar (NA) media as a comparison standard. Then the media planted with Staphylococcus aureus and Escherichia coli bacterias, it was incubated at 37oC for 1-3 x 24 hours, observed the growth of bacterial colonies, counted the number of colonies, and measured the diameter of the colony as research data. As a comparison, NA media was made and the same test bacteria were planted. The analysis of the percentage of the effectiveness of the growth of Staphylococcus aureus bacteria on various bean seeds media compared to Nutrient media: soybean medium 76.67% (less effective); mung bean 75.71% (less effective); red bean 101.79% (very effective); and peanut 99.53% (effective); whereas for Escherichia coli bacteria are: soybean 97.29% (effective); green beans 93.75% (effective); red beans 88.89% (quite effective) and peanuts 122.22% (very effective). In conclusion, various growth bean media can grow Gram-positive bacteria Staphylococcus aureus and Gram-negative bacteria Escherichia coli. Growth media of various bean seeds are effective in growing but not effective in breeding S. aureus bacteria. Growth media of various bean seeds are effective in growing and breeding E. coli bacteria which are capable of replacing Nutrient Agar (NA) media.

Keywords
alternative media, nut bean seed, bacterial growth, breeding, Staphylococcus aureus, Escherichia coli

Topic
MEDICAL LABORATORY TECHNOLOGY

Link: https://ifory.id/abstract/TmRcbE6Jt7wP

Corresponding Author
Anik Nuryati

Institutions
Politeknik Kesehatan Yogyakarta

Abstract
ABSTRAK The growth of microorganisms in the media is influenced by physical factors and chemical factors. Growth media must contain nutrients such as proteins and carbohydrates. Nuts, have a source of protein for the growth of fungi. Reshuffle bean material into simpler compounds through extrasi and germination are compounds that are good for the growth of fungi. The aim of research to determine the effectiveness of media Extraction And Soybean Sprouts Against fungus growth, and determine an effective medium. An experimental study by measuring the diameter of fungal growth for 5 days at a concentration of 2, 3, 4, 5 and 6% media seed extract and soy bean sprouts. The implementation was carried out at the Yogyakarta Health Analyst Health Laboratory, from May to September 2018. Subjects were Candida albicans, Sacharomyces cerevicea, Rhizopus Oligophorus and Aspergillus flavus, 24 hours old. The research object of local soybeans is of good quality, whole seeds, not rancid, not grained. Results, 5% soybean extraction medium was effective for the growth of Rhizopus oligosporus filamentous fungi, and 4% concentration was effective for the growth of fungi Aspergillus flavus. Media soya bean sprouts 3% effective concentration for pertumbhan fungi colonize namely Saccharomyces sereviceae, and concentration 6%, effective for the growth of the fungus Candida albicans. In conclusion, the extraction medium and soybean sprouts can be used as a substitute for the median SDA for fungi growth

Keywords
medium, extracts, sprouts and mushrooms

Topic
MEDICAL LABORATORY TECHNOLOGY

Link: https://ifory.id/abstract/wkY2TNxUbKy6

Corresponding Author
Emma Kamelia

Institutions

1Department of Oral Health Therapy, Tasikmalaya Health Polytechnic, West Java, Indonesia.
2Department of Neurosurgery, Faculty of Medicine, Hasanuddin University Makassar, Indonesia
3Molecular Biology and Immunology Laboratory for Infection Diseases, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia
4Midwifery Study Program, Medistra Health Higher school, Jakarta, Indonesia

Abstract
The objective this study is to analyze f2-isoprostane levels in the serum of a Wistar rat model of Alzheimers disease (AD) treated with Musa paradisiaca-Linn (MPL) ethanol extract or banana extract (BE). Twenty wistar rats were randomized into five groups: K0, without AD induction and no BE; K1, AD induction, no BE; K2, AD induction + BE 250 mg/kg body weight (BW); K3, AD induction + BE 500 mg/kg; and K4, AD induction + BE 1000 mg/kg. Alzheimer-s disease (AD) induction was performed by Aβ1-42 (0.2 ug) injection at the intracerebroventricullary area. F2-isoprostane level measurements were performed by Elisa kit. Paired t-test analysis showed no significant differences of f2-isoprostane serum level before Aβ induction among 5 groups (p > 0.05). At 6 weeks post- Aβ induction, there was significant increased f2-isoprostane in all groups except K0 (p < 0.05). Notably, after 3 weeks of BE administration, f2-isoprostane serum level was significantly decreased in all BE-treated groups; in the K1 placebo group, f2-isoprostane level increased. The maximum decreased f2-isoprostane level was in group K4 (-BE 1000 mg/kg BW), and minimum was in group K2 (BE 250 mg/kg BW). The results revealed that the ethanol extract of MPL fruit could decrease f2-isoprostane level in AD rat serum.

Keywords
Keywords: Alzheimer-s disease (AD), Oxidative stress, Wistar rat, F2-isoprostane, Musa paradisiaca-L (MPL)

Topic
MEDICAL LABORATORY TECHNOLOGY

Link: https://ifory.id/abstract/we7j6zfHGVMq

Corresponding Author
Michael Bhadi Bia

Institutions
Poltekkes Kemenkes Kupang; Politeknik Bina Husada Kendari

Abstract
Background: The highest case of malaria in NTT Province is Lembata District. The use of Artemisinine Combination Therapy (ACT) combined with Primakuin (PQ) in the treatment of vivax malaria aims to prevent treatment failure. The development of drug resistance and recurrence has been reported in some areas by Plasmodium falciparum. For Plasmodium vivaks no resistance has been reported to the given regimen. Evaluation of the efficacy of Dihydroartemisinin piperaquine (DHP) combined with Primakuin (PQ) has never been done. Aims: To evaluate the efficacy and side effects of DHP + PQ in the treatment of Vivax malaria in Wulandoni District, Lembata District. Methods: The subjects were vivaks malaria patients who met the inclusion criteria as stated in the WHO antimalarial drug resistance test. Clinical manifestations and side effects are monitored during the evaluation. Results: A total of 52 respondents followed the study. The age of the patients involved ranged from 2 to 76 years consisting of toddlers, children, productive and elderly (≥ 46 years). From the Mann-Whitney test results obtained p value <0.05 is a group of toddlers with productive age, a group of toddlers with old age, children with productive age and productive age with old age can be concluded there are differences in parasite density between these groups. Conclusion: There was a treatment failure as evidenced by blood tests found parasites at H3 were caused by the patient not taking medication completely. Clinical symptoms of vivax malaria patients are fever, chills and sweating accompanied by additional symptoms such as nausea, vomiting, diarrhea, headache, anorexia, aches and coughs. Side effects of DHP + PQ drugs are still in the mild category. Parasitological and clinical conditions and age have no relationship at all.

Keywords
vivaks malaria, Dihydroartemisinin Piperaquin, side effects

Topic
MEDICAL LABORATORY TECHNOLOGY

Link: https://ifory.id/abstract/vdWecfN3UhDE

Corresponding Author
Siti Nuryani

Institutions
Poltekkes Kemenkes Yogyakarta

Abstract
Guava leaves are used as medicine, among others, for burns and wounds that blister, overcome ulcers, and overcome colds. Red guava leaf extract 40 mg / ml / day and 80 mg / ml / day can reduce the number of spermatids in male white mouse models. The effect on spermatogenesis is caused by the content of guava leaf extract namely alkaloids, flafonoid, tannin, essential oil, avicullarin, oleanolic acid, saponin and beta-sitosterol. Saponins have an effect especially on cells that develop like during spermatogenesis and oogenesis. The study aimed to determine the effect of guava leaf extract on the process of oogenesis, especially on primary, secondary, degraf and atresia follicles. Method : pre-experimental study used guava leaf extract 4mg / ml which was given orally every day to adult female mice for 4 weeks. A total of 10 mice were divided into 2 groups of 5 each. The two groups were K for control and P for treatment. Each of the mice was sacrificed for their ovaries. The tissue processed by the Paraffin method was then prepared and stained with Hematoxylin Eosin. to be assessed for the number of primary follicles, secondary , degraf and atresia follicles. The data analyzed descriptively The results of the study are as follows: the average number of follicles in normal animals without treatment is as follows primary 58, secondary 18, degraf 4 and atresia 48. Animals treated (P) average primary follicles 38, secondary 24, degraf 2 and atresia 61 Conclusion: The administration of methanol extract of guava leaves with a dose of 4mg / ml daily for 4 weeks affected the number of primary follicles, secondary, degraf, and atresia follicle

Keywords
methanol extract, guava leaves (Psidium guajava L.), oogenesis

Topic
MEDICAL LABORATORY TECHNOLOGY

Link: https://ifory.id/abstract/MxbdFvkHnPhB

Corresponding Author
FURAIDA KHASANAH

Institutions
Poltekkes Kemenkes Yogyakarta

Abstract
Examining the Activated Partial Thromboplastin (APTT) and Plasma Protrombin Time (PPT) is sort series of examining homeostasis which is conducted in order to have screening test for homeostasis disorder. This examination used plasma sample in which there where solidification factors which could be influenced by thrombocyte existence. The centrifuging of citrate blood sample which was conducted too fast or too slow would cause plasma condition with the number of thrombocyte. Practical in some laboratories were not yet been uniformed, mainly in centrifuging of citrate blood to obtain citrate plasma with less thrombocyte contents. To identify the value of Kappa by compatibility test between two methods PRP and PPP to examine PPT and APTT. This experiment is true experiment with post test research design without control. Citrate plasma sample was obtained from 10 sample of students- blood which had one pair, 2 treatments; by centrifuging 3000 rpm for 10 minutes for PPP and centrifuging 1000 rpm for 10 minutes for PRP. Then, both methods (PPP and PRP) were examined by using PPT and APTT parameter. In the result of PPT examination in the sample of PRP plasma, the average value was 11.6 seconds. In the sample of PPP, the average value was 11.0 seconds. The result of APTT examination in PRP sample, the average value was 34.27 seconds while in PPP sample was 33.18 seconds. There were compatibility in the result, either PPP method and PRP for PPT and APTT examination (Kappa = 1).

Keywords
PRP, PPP, APTT, PPT, Kappa.

Topic
MEDICAL LABORATORY TECHNOLOGY

Link: https://ifory.id/abstract/h9XPKVrzWCNG

Corresponding Author
Ni Made Susilawati

Institutions
Department of Technology Laboratory Medic, Politekkes Kemenkes Kupang

Abstract
Antibiotics are a group of compounds that can inhibit bacterial growth (bacteriostatic) or cause of death bacterial (bactericidal). Bacterial resistance occurs due to inappropriate administration of antibiotic doses and mis of diagnosis by health workers. Bacteria that are resistant to penicillin, cephalosforin and aztreonam generally have an enzyme released to defend themselves, this enzyme is called Extended Spectrum Beta Lactamase (ESBL). This study aims to determine the number of gram-negative bacteria that produce Extended Spectrum Beta Lactamase (ESBL) which is examined from samples that are cultured in the microbiology section in the laboratory of RSUD Prof.DR.W.Z. Johannes Kupang from 2016 to 2018. Secondary data were taken, processed and presented in descriptive form. The Bacteria Extended Spectrum Beta Lactamase (ESBL) was the most found is 104 (50.9%) Klebsiella pneumoniae bacteria, followed by 56 (29.78%) Escherichia coli bacteria, and Enterobacteriaceae (3.61%). ESBL group bacteria mostly came from the Neonatal Intensive Care Unit (NICU) room as many as 26, ESBL group bacteria were mostly found in pus samples as many as 33 (19.41%).

Keywords
Extended Spectrum Beta Lactamase (ESBL, RSUD Prof.DR.W.Z. Johannes Kupang

Topic
MEDICAL LABORATORY TECHNOLOGY

Link: https://ifory.id/abstract/Fh4wmDyLqTPe

Corresponding Author
Norma Tiku Kambuno

Institutions
1) Department of Medical Laboratory Technic , Poltekkes Kemenkes Kupang
2) Department of Pharmacist , Poltekkes Kemenkes Manado
3) Department of Nurse Dental, Poltekkes Kemenkes Semarang

Abstract
Background: Hepatitis B caused by hepatitis virus infection B (HBV) is a world health problem including Indonesia. Results of 2013 Basic Health Research estimated that there were 1,2% of the population in Indonesia with Hepatitis and East Nusa Tenggara was the province with the highest Hepatitis B prevalence (4,3%). Transmission can be through vertical (perinatal) and horizontal (house contact). Aims; The purpose of this study was to analyze the risk factors for transmission at home contact from students with HBsAg reactive. Methods; This study was performed in two phases. The first phases, November 2015, examination of HBsAg status with ELISA sandwich method was conducted on 341 students of Senior High School Kupang city obtained 45 samples with HBsAg reactive. The second phase, June 2016, out of 23 students HBsAg positive cases and 89 samples of family members were enrolled and evaluated. Of family members, 18 samples were found with HBsAg reactive, 6 subjects (33%) were fathers, 4 subjects (22%) were mothers, 5 subjects (28 %) were sisters and 2 subjects (11%) were brothers and 1 subject (6%) were others family members. Statistical analysis with Chi Square (X2) and Odds Ratio. Results: There is no significant relationship between HBsAg status with sex and age. There is significant relationship between HBsAg status with education, and marital status. The values were obtained (OR=0,184, 95%, Cl; 0,059-0,579) for education and (OR=0,204, 95%, Cl; 0,07-0,597) for marital status. Conclusion; The presence of family members with reactive HBsAg can transmit to other family members, 25% of family members also detected reactive HBsAg. More attention should be paid to HBV vaccination for family members of HBV patients.

Keywords
Risk Factors, Home Contact, HBsAg Reactive

Topic
MEDICAL LABORATORY TECHNOLOGY

Link: https://ifory.id/abstract/TayvA3t8YCqn

Corresponding Author
Agustina Wilhelmina Djuma

Institutions
1, 2,3) Department of Medical Laboratory Technic, Poltekkes Kemenkes Kupang

Abstract
Background: Worm infection is one of the health problems in all countries, including Indonesia. The most common worm infection is infection caused by Soil Transmitted Helminth (STH) group. Primary school are the age group who are susceptible to infection with intestinal worms. Worm infections can have a serious impact if it is not treated with the administration of deworming drugs and a clean and healthy lifestyle of the person. Aims: This research was conducted with the aim of finding out risk factors such as the characteristics of children and parents, childrens behavior, and environmental sanitation related to the incidence of STH infection in children aged 6-12 years in Dusun II and IV of Manusak Village, Kupang Timur District, Kupang Regency in 2019. Method: This research uses observational analytic research with cross sectional design. The research subjects were 56 children. Data were collected using questionnaires then analyzed using Chi Square test (X2). Results: Worm examination is done by the Kato- Katz type indirect method. Based on the results of Kato-Katz examination, eggs of Ascaris lumbricoides were found in 54 children (96.4%). Conclusion: The Chi Square test results showed that the risk factor for STH infection was the habit of not washing hands with (P <0.05).

Keywords
Characteristics, Behavior, Sanitation

Topic
MEDICAL LABORATORY TECHNOLOGY

Link: https://ifory.id/abstract/y7Px8VpWFUaR

Corresponding Author
Muji Rahayu

Institutions
Politeknik Kesehatan Kemenkes Yogyakarta

Abstract
STUDY OF EFFICACY LEMONGRASS (Cymbopogon citratus) EXTRACT AS LOWERING BLOOD CHOLESTEROL LEVEL AGENT ABSTRACT Muji Rahayu, Sistiyono hayuningpuji@gmail.com Lemongrass (Cymbopogon citratus) is a rich plant containing compounds called polyphenols, especially flavonoids. Lemongrass has been widely used in many counytries as antiinflamation, antibactaria, antifungal, antidiarhoea and as repellent to insect also proven to lower blood glucose. This study aims to determine the effect of the ethanol extract of lemongrass on blood lipids profile using 25 male albino rats Wistar strain, were devide into 5 groups, each group consisted of 5 rats, namely the control group (K), the drugs standard Simvastatin group (Sim) and the treatment group ES100, ES200 and ES400. All rats made hyperlipidemic by administration of diet high in fat and egg yolk for 14 days, then assayed lipid profile include total cholesterol, LDL, HDL and triglyceride (pre test). Furthermore, the group K were without treatment, S group were give drug standard simvastatin 0,18 mg/200g bwt, ES100, ES200 and ES400 group treated with ethanol extract of lemongrass respectively 100, 200 and 400 mg/kg bwt with sonde for 14 days. On the 15th days all rats were fasted and then have blood drawn through orbital venous and made serum and defined serum lipid profile (post test). The result showed the group of rat treated with ethanol extract decreased the levels of total cholesterol, LDL, and triglyceride as well as an increase in HDL cholesterol levels were significantly (p<0.05). This study showed the ethanol extract of lemongrass has lowering cholesterol levels and improve lipid profile in hyperlipidemic rats.

Keywords
Cymbopogon, cholesterol, LDL, HDL, triglyceride

Topic
MEDICAL LABORATORY TECHNOLOGY

Link: https://ifory.id/abstract/T3PFGhJxC7Mn

Corresponding Author
Subrata Tri Widada

Institutions
Health Polytecnic Yogyakarta

Abstract
Kidney is a important organ in the human body, the body will have problems if the kidney does not work properly. Treatments for the disease may include hemodialysis. Patient of hemodialysis is often found high creatinine, ureum, and uric acid levels. This condition may affect the quality of laboratory results. According to The Beer-Lambert-s Law, the portion of the light absorption will depend on how many molecules it interacts with. However, the theory cannot be applied to a solution with high concentration. This condition can trigger inaccurate results which may be harmful to the patients because it cause errors in diagnosis, treatment and disease monitoring. This condition can be handled by diluting the concentrated serum. A serum sample was diluted with a physiological saline solution (NaCl). This study aims to determine the level of kidney function test in hemodialysis patients serum with and without dilution. This research was Pre-experimental Design methods with One-Group Pretest-Posttest Design. Research sample was used serum of hemodialysis patient with creatinine level more than 10 mg/dL, ureum level more than 256 mg/dL, and uric acid level more than 12mg/dL counted 40 samples. The test results were analyzed descriptively and statistically using Paired Sample T-Test. The mean of creatinine and uric acid levels increase was 2.29 mg / dL (16.72%) and 4.02 mg / dL (44.47%), while the mean urea decreased was 19.52 mg / dL (6.38 %).The results tested with Paired Sample T-Test showed a significant 0.000 (<0.05) which means there was a difference of kidney function test in hemodialysis patients with and without dilution.

Keywords
Kidney Function Test, Hemodialysis, Without Dilution, Dilution.

Topic
MEDICAL LABORATORY TECHNOLOGY

Link: https://ifory.id/abstract/LrjkBa8zJcKM