Indonesia Conference Directory

Corresponding Author
Nor Azlina Ahmad

Institutions
Department of Biosciences,
Faculty of Science,
Universiti Teknologi Malaysia (UTM),
81310 UTM Johor, Malaysia

Abstract
Current analytical characterization of phytochemical is expensive and laborious. Alternatively, techniques such as biosensor with high binding affinity and specificity to the target such as aptamer emerged recently. Aptamers are oligonucleotides with structured molecules that can bind to their targets by specific 3-D conformation. Though aptamer technology have taken place for the past 30 years, little works have been conducted on its capacity to bind phytochemical compounds. Here, DNA aptamers named APT1 to APT30 3-D structures and possible binding mechanism of the aptamers-quercetin complex were evaluated in-silico. Secondary and tertiary structure of 28 potential DNA aptamers candidates were modelled adopting MFold and RNA Composer, respectively. The correspond sequence further modified from RNA to DNA by Discovery Studio Visualizer software. Applying AutoDock Vina, the aptamer-quercetin interactions were predicted. Amongst the aptamers, APT14, APT15 and APT1 displayed highest binding affinity with -9.4, -9.3 and -9.3 kcal/mol, respectively. Upon structure analysis, most of the binding sites that possess high docking energy were positioned at loops and stem conformation. The later plays an important role in stabilization while the bases within the loop motifs responsible in supporting the binding. The stability of potential aptamers-quercetin complex will be further explored for their specific intermolecular interaction using molecular dynamics simulation.

Keywords
DNA aptamers, 3-D structure, quercetin, binding affinity, molecular interaction

Topic
Bioinformatics

Link: https://ifory.id/abstract/YK6kFyNfaBpU

Corresponding Author
Yuslinda Annisa

Institutions
Universitas Negeri Malang

Abstract
The tribes in Indonesia are vary. Tengger tribe is one of the Indonesian tribes that located in Bromo Tengger Semeru NationalPark (BTSNP). BTSNP area has a very high diversity of plants. Various types of plants were used by Tengger Tribe in any field such as medicine, food sources including animal feed, industry and households, building materials, ecology, and traditional rituals. Based on interview with key informant called ‘Dukun- and Tengger tribe local people were found that Physalis angulata (local name: Ciplukan gunung) is the most used within Tengger tribe. They used P. angulata to treat any kind of diseases especially keep heart and lung in healthy condition. Tengger tribe used P. angulate by boiling in certain time then drink it as a tonic. The aims of this research were to analyze active compound from decoction of P. angulate that has anti-inflammatory agent. High Performance Liquid Chromatography (HPLC) result showed that decoction of P. angulate contained with active compound, one of them was Benzaldehyde. In silico study between benzaldehyde and cyclooxygenase 1 (COX-1) resulted that benzaldehyde has potency as inhibitor for COX-1. COX-1 is involved in inflammatory pathway. The inhibition towards COX-1 resulted on decreasing of inflammation. P. angulate has function as anti-inflammation.

Keywords
Tengger tribe, Bromo Tengger Semeru National Park (BTSNP), Physalis angulata, anti-inflammation, Cyclooxygenase 1 (COX-1)

Topic
Bioinformatics

Link: https://ifory.id/abstract/83VEhDkXzmru

Corresponding Author
Erna Sulistyowati

Institutions
University of Islam Malang (UNISMA)

Abstract
Background: Green tea leaf (Camelia sinensis) possess certain antibacterial compounds. Recent studies have been presented that C sinensis has critical roles in bacterial breakdown proccess. This study identified the antibacterial activity of C. sinensis leaf extract against Staphylococcus aureus in combination with cotrimoxazole. Methods: Methanolic or decoction extract of C sinensis were determined. The antibacterial activities (single or in combination with cotrimoxazole) were measured with zone of inhibition (ZOI) assay through agar well diffusion method. These ZOI were evaluated with Ameri-Ziaei Double Antibiotic Synergism Test (AZDAST). The data was analyzed with Kolmogorov-Smirnov, followed by a non-parametric Mann-Whitney Test. Significant level was at p<0.05. Results: The ZOI-s diameter of S. aureus on combination of C. sinensis methanolic extract with cotrimoxazole, C. sinensis decoction with cotrimoxazole, low dose of cotrimoxazole, single high dose of C. sinensis methanolic extract, single low dose of C. sinensis methanolic extract, and C.sinensis decoction were 25.67 ± 5.8 mm, 25.67 ± 1.53 mm, 10.33±0.58 mm, 9.67±0.58 mm, 8.67±0.58 mm, and 0 mm, respectively. Combination of C. sinensis methanolic extract with cotrimoxazole and C. sinensis decoction with cotrimoxazole were significantly increased ZOI-s diameter (p<0.05). Combination of C. sinensis methanolic extract and cotrimoxazole has synergistic effect, whereas C. sinensis decoction with cotrimoxazole showed potentiation effect. Conclusion: Combination of C. sinensis leaf extract and cotrimoxazole is potential as an antibacterial agent.

Keywords
Camellia sinensis, Cotrimoxazole, Staphylococcus aureus, antibacterial assay

Topic
Biomedical Science

Link: https://ifory.id/abstract/Bd9bMR4nq8Gm

Corresponding Author
Hendra Susanto

Institutions
1) Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Malang, Indonesia
2) Faculty of Medicine, Universitas Gadjah Mada, Indonesia
3) Department of Digestive Surgery, Dr. Sardjito Hospital, Yogyakarta, Indonesia
4) Department of Nursing Sciences, Tribhuwana Tunggadewi University, Indonesia

*Corresponding Author-s email: hendrabio[at]um.ac.id

Abstract
Liver fibrogenesis can turn out into liver cirrhosis and being a fatal failure. Infection of HBV and metabolic syndrome is the major causes of liver fibrogenesis. ANGPTL8 is the hormones that interact with many factors related to HBV infection and metabolic syndrome. The purpose of this study was to crosscheck computational based interaction of ANGPTL8 and TGFβ1 on TGFβR1 as the pathological signal that related to liver fibrogenesis. Our study shows that ANGPTL8 bind better onto TGFβ1R than TGFβ1 so that ANGPTL8 may implicate with fibrogenesis through simultaneous binding into TGFβ1R with TGFβ1. This study work as an initial study that can be used as the basis of the next study to determine the role of ANGPTL8 in the occurrence of liver fibrogenesis due to metabolic syndrome and HBV infection.

Keywords
ANGPTL-8, liver fibrinogenesis, TGFβ1R, hepatocellular carcinoma

Topic
Bioinformatics

Link: https://ifory.id/abstract/WbUYpk3m6hgr

Corresponding Author
Islakhul Kharoir

Institutions
Department of Biology Education, University of Muhammadiyah Malang, Jl. Raya Tlogomas No. 246 Malang 65144, East Java, Indonesia

Abstract
The development of the use of preparations with natural coloring has been carried out a lot, but there is no one that shows optimal concentration. The purpose of this study was to determine the optimal concentration of Erythrina crista-galli L. flower extract as a natural dye based on the parameters of the quality and stability of the preparation. The type of research used is experimental research. This research method through several stages: extracting, diluting extracts with various concentrations (30%, 50%, 70%, 90%, and 100%), until making maceration preparations. Data collections were conducted twice, where the first and second take has an interval of one semester. Kruskall Wallis is used as a technique for analyzing data from research results. Both in the first and second observations, the concentration of 70% always produce the best quality preparations. Thus the Erythrina crista-galli L. flower extract has the potential as natural staining in histological preparations.

Keywords
Erythrina crista-galli L., natural staining, preparation histology

Topic
Botany

Link: https://ifory.id/abstract/4E3f7BcbkxHW

Corresponding Author
Siti Zubaidah

Institutions
(a) Biology Education, Faculty of Mathematics and Natural Sciences, Universitas Negeri Malang, Jl. Semarang No. 5, Malang 65145, Jawa Timur, Indonesia
(b) Indonesian Legume and Tuber Crops Research Institute, Indonesian Agency for Agricultural Research and Development. Jl. Raya Kendalpayak Km. 8 Malang 65101, East Java, Indonesia

Abstract
A character that is inherited from parents to offspring is influenced by gene action. Genes that control plant character can be influenced by additive, epistatic or dominant gene action. The purpose of this study was to determine the inheritance of the morphological characters of leaves, pods and soybean seeds through analysis of gene action and the number of genes in F2 populations of UM 3.2 x Argomulyo and UM 6.1 x Burangrang cross. The experiment was carried out at Indonesian Legume and Tuber Crops Research Institute (Iletri) Kendalpayak, Malang, Indonesia. Estimating the gene action and the number of genes was done by measuring the value of skewness and kurtosis. The suitability of inheritance patterns was analyzed by using the chi-square test. The results showed that the character of leaf length, leaf width, pod thickness and seed hilum length in the UM 3.2 x Argomulyo cross were influenced by the additive genes action with complementary epistatic influences while the character of the petiol length, pod length, pod width, seed length, seed width, and seed thickness was affected by additive gene action with duplicate epistatic effects. In UM 6.1 x Burangrang cross, the characters of leaf width, pod thickness, seed width, seed thickness and length of seed hilum were influenced by the additive genes action with complementary influences while the characters of leaf length, petiole length, pod length, pod width, and seed length were influenced by additives gene action with duplicate epistatic influence. The characters of petiol length, pod length, pod width, pod thickness, seed length, seed width, and seed hilum length were controlled by many genes.

Keywords
gene actions, number of genes, morphological of soybean

Topic
Botany

Link: https://ifory.id/abstract/jXv7AmrwcFEC

Corresponding Author
Wira Eka Putra

Institutions
1Departement of Biology, Faculty of Mathematic and Natural Sciences, Universitas Negeri Malang
2Departement of Biology, Faculty of Mathematic and Natural Sciences, Universitas Brawijaya
3Division of Genetics and Molecular Biology, Yayasan Generasi Biologi Indonesia (Genbinesia)

*Corresponding Email: hendrabio[at]um.ac.id

Abstract
Nuclear factor kappa B (NF-κB) is known as master of regulation due to its multiple roles in biological system including metabolism, inflammation, or even diseases like cancer. The downstream phosphorylation of NF-κB complex is affected by the activity of IKK-B. Thus, in this study we aims to evaluate the bioactive compounds of Zingeber officinale as inhibitor agent against the IKK-B. The virtual screening evaluation was based on the interaction activity and binding free energy among the protein and the ligands. The virtual chemical structure and protein structure were obtained from pubchem web server and PDB respectively. Molecular docking, visualization, and data analysis were performed by computation approach. In this study, we found that 6-Shogaol might potential as drug like molecule for anti-inflammation compared to other compounds. Importantly, further study needed to evaluate the biological mechanism of 6-Shogaol inhibit the IKK-B activity.

Keywords
Anti-inflammation, IKKB, virtual screening, Zingiber officinale

Topic
Bioinformatics

Link: https://ifory.id/abstract/eqkjmdB3hELr

Corresponding Author
Danial Mursyd

Institutions
1) Student Postgraduate Department of Biology Education, Faculty of Math and Science, University of Malang, Indonesia
2) Department of Biology, Faculty of Math and Science, University of Malang, Indonesia
3) Department of Geography, Faculty of Sosial Science,University of Malang, Indonesia

a) fatchur.rohman.fmipa[at]um.ac.id
b) mursyddanial94[at]gmail.com

Abstract
Research on insect communities was carried out in different zones in the monsoon forest in Baluran National Park, Situbondo, East Java. This study aims to identify and describe the composition of taxa, the level of diversity, evenness and wealth of taxa in sheltered areas and exposed to the monsoon forest of Baluran National Park. The study was conducted from July to October 2018. Sampling was done by purposive sampling technique with the direct sweeping method, light trap method, and pitfall trap method. The results showed that the composition of insect taxa in both areas had moderate diversity criteria with index values in the protected area 1.72 and exposed area 1.79. The taxation evenness index values in the two areas are classified as evenly with the area evenness index being 0.51 and the exposed area 0.59. The tax wealth index value in the sheltered area is 3.52 and the exposed area is 2.9 with the category obtained being medium.

Keywords
Insect Communities, Monsoon Forest, Baluran National Park

Topic
Bioconservation

Link: https://ifory.id/abstract/rz6HZqY4XfkK

Corresponding Author
Vivi Novianti

Institutions
Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Malang

Abstract
The inhibitory potential of plants during early succession is crucial but little appreciated. Negative effects of these species can slow, arrest, or deflect succession. Aggressive invaders suppress plantings or nurse plants suppress desired target species. Such an inhibitory effect often causes problems during restoration. Study aggressive invaders would assist in informing ecological restoration. Here, line transects was used to investigate species composition and its characteristic on seven coal mine overburden dumping site in tropical climate. There are 124 plant species invaded coal mine overburden on early succession, and 70 of them are classified as invasive species, such as Acacia mangium, Chromolaena odorata, Centrosoma pubescens, Micania micrantha, Imperata cylindrica, and Lantana camara. Acacia mangium and Centrosoma pubescens which are widely planted for reclamation purposes on ex-coal mine area categorized invasive species. The negative impact of these species on inhibition of succession has been seen in certain location. Therefore, understanding the nature of species can facilitate the development of target species for accelerating succession.

Keywords
Invasive plants, overburden spoil, coal mine, succession, ecological restoration

Topic
Environmental Engineering

Link: https://ifory.id/abstract/UqY9tfMdZVpW

Corresponding Author
ATIKAH AMALIA

Institutions
Biology Department, Faculty of Mathematics and Natural Sciences, Universitas Negeri Malang, Indonesia

Abstract
It has been investigated that the prevalence of Rheumatoid Arthtritis (RA) is 0.5% -1.0% in general population worldwide. Inflammation arises because of prostaglandins, which are proinflammatory mediators enacted by the stimulus. The key enzyme in inflammation is cyclooxygenase-2. Flavonoids have various biological effects in mammalian cells carried out both in vitro and in vivo. This study was carried out to predict the potential of flavonoid group compounds as anti-inflammatory agents for rheumatoid arthritis through virtual screening using molecular docking methods. This method was employed to predict the potential of flavonoid compounds using web server PASS SERVER, predictions of the pharmacokinetic properties of flavonoid compounds including absorption, distribution, metabolism, excretion, and toxicity using pkCSM and molecular docking web servers to determine the interaction of COX-2 enzyme flavonoid compounds which was compared with control drugs. The results of this study indicated that flavonoid compounds (kaempferitrin, afzelin, and routine) could potentially replace the drug rofecoxib based on potential compounds, namely as anti-inflammatory, non-steroidal anti-inflammatory agents, antioxidants, and anti-carcinogenic. Flavonoid compounds had a decent ADMET profile. The compound had considerable activity as an anti-inflammatory agent based on its interaction with the COX-2 enzyme as indicated by the binding affinity value of ± -8.5 kcal/mol.

Keywords
Flavonoids, Inflammation, Molecular Docking

Topic
Bioinformatics

Link: https://ifory.id/abstract/NAByGmCu8Xxc

Corresponding Author
Suharti Suharti

Institutions
a)Department of Chemistry, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Negeri Malang, East Java, Indonesia
b)Department of Chemistry, Faculty of Science, Universiti Malaya, Kuala Lumpur, Malaysia

Abstract
Keratin is an important biomaterial for industrial applications. About 90% of keratin is found in chicken feathers. Chicken feathers are accumulated from poultry processes and produced in millions of tons annually. Besides being abundant and cheap, keratin is insoluble in water and organic solvent which makes it difficult to recycle. Previous study showed that solid-state fermentation of keratinase using Bacillus sp. MD24 using chicken feathers a sole carbon and nitrogen source produced micro-keratin as a solid by-product. This paper reported our investigation in soluble keratin in liquid by-product. Liquid byproduct was produced through Solid-State Fermentation of keratinase for 10 consecutive days by Bacillus sp. MD24. Liquid by-product was separated from solid by-product by centrifugation at 2000 rpm. The size of soluble keratin was examined by zeta particle analyzer. The soluble keratin was filmed and the film was characterized using Fourier Transform Infrared Spectroscopy (FTIR), and film surface was analyses using Scanning Electron Microscopy (SEM). Dissolution of keratin using ionic liquids ([BMIM])Cl and ([EMIM])Ac was done to compare its properties. Dissolution was performed under nitrogen atmosphere at 130 °C for 7 hours. Insoluble fraction was recovered by addition of water. The results showed the liquid by-product contains soluble keratin with ([EMIM])Ac is 1085,78 ± 359,7723 nm; ([BMIM])Cl is 1311,88 ± 573,0562 nm and SSF is 2677,4 ± 611,4363 nm. The FTIR spectrum indicated the presence of polypeptide with high concentration of S-H bond. There is no difference on surface structure between keratin biofilm produced from soluble keratin by-product and soluble keratin produced by dissolution using ionic liquid.

Keywords
keratin, Solid-State Fermentation, Ionic Liquids

Topic
Biomaterial Science

Link: https://ifory.id/abstract/pGHXCUPBZjwu

Corresponding Author
Shelby Amrus Ernanda

Institutions
1Master Program in Biomedical Science, Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia.
2Department of Orthopaedics and Traumatology, Faculty of Medicine, Universitas Brawijaya, dr. Saiful Anwar General Hospital, Malang, Indonesia.
3Doctoral Program in Medicine, Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia.
4Medical Doctor Study Progam, Faculty of Medicine, Universitas Brawijaya, Indonesia
5Pharmacy Study Program, Faculty of Medicine, Universitas Brawijaya, Indonesia
6Department of Cardiology and Vascular Medicine, Faculty of Medicine Universitas Brawijaya, dr. Saiful Anwar General Hospital, Malang, Indonesia

Abstract
Menopause is a condition where a woman lost protection from estrogen, causing susceptibility to cardiovascular disease. Such condition is caused by narrowing of arterial lumen induced by increased Reactive Oxygen Species (ROS) activity and inflammation. It was reported that nicotine can reduce ROS production and act as anti-inflammation agent. Therefore, we conducted a research to explore the possibility of using nicotine to prevent arterial wall thickening and to reduce free radical activity. We did in vivo true experimental and randomized post-test only controlled group research in 18-20 weeks old Rattus norvegicus. We examine tail arterial wall thickness, lumen radius, and Wall to Lumen Ratio (WTLR), as well as Malondialdehyde (MDA) level as marker of ROS. The first group was normal rats (Sham). The second group represent ovariectomy group (ovariectomy-only/OVX). The third to fifth group was ovariectomized and given nicotine in dose of 0.25 mg/kg body weight (P1), 0.5 mg/kg (P2), and 0.75 mg/kg (P3). Group P1-P3 was given daily nicotine by oral route for 4 weeks, started from 3 weeks post ovariectomy. After treatment period was completed, needed organ was harvested. MDA levels of P3 were significantly lower than Ovx group (p = 0.036, Tukey HSD). P2 groups showed significantly thinner arterial wall (intimal-medial layer) than the Ovx group (p = 0.001, Tukey HSD). No significant different in lumen radius between groups was observed. WTLR measurement showed that P1, P2, and P3 has 6%, 42%, and 3% lower WTLR compared to Ovx group, respectively. Finally, we conclude that nicotine administration can prevent tail arterial wall thickening and has antioxidant Property.

Keywords
Arterial Wall Thickness, Malondialdehyde, Nicotine, Menopause, Ovariectomy

Topic
Biochemistry

Link: https://ifory.id/abstract/ZnNrCKFwYpcB

Corresponding Author
Nia Lukita Ariani

Institutions
a) Department of Nursing Science, Tribhuwana Tunggadewi University, Malang, East Java, Indonesia 65144
*nia.lukita[at]unitri.ac.id

b) Department of Biochemistry, Faculty of Medicine, Gadjah Mada University, Yogyakarta, Indonesia 55281

Abstract
Adiponectin has a protective role in metabolic syndromes. Adiponectin gene polymorphism +276G>T (rs1501299) was reported has a strong relationship with increased adiponectin level. However, reports about this variant are still inconsistent in obese people. We investigated the association of adiponectin gene variant with circulating adiponectin. The study was performed with 110 apparently healthy adults about 20-40 years old Javanese people. Pregnant subjects and subjects either under diabetic or other metabolic syndrome medications, were excluded. Plasma was used to measure 8- hours fasting glucose and adiponectin level. PCR-RFLP examined DNA genotyping. The data were analyzed using one-way ANOVA, Fisher Exact test, independent t-test, and Mann Whitney test (p<0.05). Genotyping results showed three genotypes of this polymorphism, namely GG, GT, and TT. No significant difference in genotype distribution related to circulating adiponectin both in obese and lean group. Despite the fact about adiponectin lowering action in the obese group, this polymorphism may neither a risk factor nor protective variant for the Javanese population. These findings assert the adiponectin gene variant still vary in influencing circulating adiponectin among the different population.

Keywords
ADIPOQ gene, circulating adiponectin, metabolic syndromes, obesity, SNP +276G>T (rs1501299)

Topic
Biomedical Science

Link: https://ifory.id/abstract/LQTAKtaeXp7f

Corresponding Author
Tri Retnaningsih Soeprobowati

Institutions
1) Ph.D Program of Environmental Studies, School of Postgraduate Studies, Diponegoro University, Semarang, Indonesia
2) Department of Biology, Faculty of Mathematics and Natural Sciences, State University of Semarang, Indonesia
3) Faculty of Fisheries and Marine Sciences, Diponegoro University, Semarang, Indonesia
4) Department of Biology, Faculty of Sciences and Mathematics, Diponegoro University, Semarang, Indonesia
5) School of Postgraduate Studies, Diponegoro University, Semarang, Indonesia

Abstract
Abrasion that had occurred since 20 years ago in Bedono Village, Demak, Central Java due to land conversion from mangrove ecosystem to shrimp ponds. Mangrove ecosystem have an important role in coastal area. This study proposed to analyze mangrove conservation executed by the society and its significance on community life of Bedono Village, Demak, Central Java. Data involves some activities, planting area, and the number of mangrove species planted revealed from secondary data, society response by in-depth interviews and supporting data by observation. Based on the analysis, it is known that mangrove planting area during 2004-2015 reached 382.4 Ha with 922,570 mangrove trees planted. Need more than 10 years to achieve the program, by empowering and the active role of the community. Furthermore, mangrove conservation provides both direct and indirect favor for society.

Keywords
abrasion, mangrove conservation, community life, Bedono, Demak

Topic
Bioconservation

Link: https://ifory.id/abstract/heBNaTJxzfc4

Corresponding Author
Siti Zubaidah

Institutions
(a) Biology Education, Faculty of Mathematics and Natural Sciences, Universitas Negeri Malang, Jl. Semarang No 5, Malang 65145, Jawa Timur, Indonesia
(b) Indonesian Legume and Tuber Crops Research Institute, Indonesian Agency for Agricultural Research and Development. Jl. Raya Kendalpayak Km. 8 Malang 65101, East Java, Indonesia

Abstract
Soybean is an important food crop in Indonesia. One of the major problems in soybean production was a virus infection, with CpMMV as one of the most important viruses. The process of plant breeding to produce virus resistant soybeans and high productivity can be done by crossing virus resistant line with superior varieties. Important information in plant breeding program development of resistant varieties and high-productivity was to know the genetic of the trait, one of which is the maternal effect. The purpose of this study was to analyze the inheritance of maternal effects through the agronomical characters of F1 soybeans derived from the crossing of the Panderman variety and the CpMMV-resistant soybean lines. The experiment used a randomized block design with three replications. The results showed that the maternal effect was found out on the seed yield per plant for Panderman x UM61. Five crosses showed the maternal effect on the weight of 100 seeds namely Panderman x UM24, Panderman x UM34, Panderman x UM61, Panderman x UM63, and Panderman x UM73. Maternal effect on plant height was only found out in Panderman x UM62 cross. The maternal effect was also found on the number of unfilled pods in Panderman x UM34 and Panderman x UM61 crosses.

Keywords
maternal effect, agronomic, soybean

Topic
Botany

Link: https://ifory.id/abstract/qpGAFRmL9ENh

Corresponding Author
Siti Halimah Larekeng

Institutions
Biotechnology and Tree Breeding Laboratory, Faculty of Forestry, Universitas Hasanuddin, Makassar, Indonesia
2 Indonesian Tropical Fruit Research Institute, Jl. Raya Solok, Aripan Km. 8, PO. Box 5 Solok, West Sumatera, Indonesia
3 Faculty of Agrotechnology, Halu Oleo University, Kendari, Indonesia

Abstract
Pollen dispersal can be used to design and manage seed orchards of Mahogany to fit the breeding demand. Pollen dispersal and mating system in mahogany can be elucidated through pollen dispersal pattern analysis based on genetic structure. Microsatellite is a co-dominant genetic marker that has been widely applied for DNA analysis. The objective is this work were to to determine specific primers for parentage analysis in mahogany, to calculate the distance of pollens travel from donor pollen to female recipient, to estimate the frequency of pollination, and to evaluate the proportion of selfing and outcrossing in mahogany. The field activity was done by collecting leaf samples at genetic resource area of Sulawesi BPTH, South Sulawesi, Indonesia. The laboratory activities for genetic molecular analysis were conducted at Biotechnology and Tree Breeding Laboratory, Faculty of Forestry, Hasanuddin University, Indonesia. Results: The study indicated that the suitable primers for parentage analysis were sm05, sm18, and sm48. Pollens could travel up to 83 m apart from the evaluated female parents, and the highest frequency of pollination was 22 at 20 to 30 m. The proportion of outcrossing was 95% which indicated that mahogany trees in this evaluated area tend to outcross pollinate. Highly variable microsatellite markers have facilitated a direct genetic approach to measuring gene flow based on parentage analysis.

Keywords
Gene flow; Mahogany; Mating system; Microsatellite; Pollen dispersal

Topic
Biotechnology

Link: https://ifory.id/abstract/akTJBtZYGEyp

Corresponding Author
Vera Budi Lestari Sihotang

Institutions
Herbarium Bogoriense Research Center for Biology Indonesian Institute of Sciences

Abstract
This study reveals medicinal plant garden as a form of community initiatives in the Selang Pangeran area, Bahorok Subdistrict, Langkat Regency, North Sumatra Province in conserving medicinal plants. This initiative is also part of the communitys efforts to provide services to the community and document the knowledge of medicinal plants. Interviews and direct collection of medicinal plants found in the medicinal plants garden are carried out. In addition, also carried out recording the name of the area, making voucher specimens to determine the type and scientific name of the plant. For each of the plants found, its use and which parts are used from the plant are recorded. Documentation of several types of plants was also made to facilitate the identification process. A total of 34 types of medicinal plants were collected for information. It can be seen that community initiatives have succeeded in preserving medicinal plant resources, documenting the knowledge of medicinal plants, and transmitting knowledge of the use of medicinal plants.

Keywords
medicinal plants, medicinal plant garden, conservation strategies, community initiatives.

Topic
Botany

Link: https://ifory.id/abstract/JEGYme6nWwhK

Corresponding Author
Nur-aini Kartikasari

Institutions
1 Department of Biology, Faculty Mathematics and Natural Sciences, Universitas Negeri Malang, Jalan Semarang No.5, Malang, 65145, Indonesia.
2 Faculty of Biology, Universitas Gadjah Mada, Jalan Teknika Selatan, Sekip Utara, Yogyakarta, 55281, Indonesia.
a)Corresponding author: nuraini.kartikasari.fmipa[at]um.ac.id
b) yekti[at]ugm.ac.id
c) lsembiring[at]ugm.ac.id

Abstract
Study of endophyte bacteria from Purwoceng roots (Pimpinella pruatjan Molk.) lead to characterized endophyte bacteria that can produce the same secondary metabolite compound as its host. Secondary metabolite from this bacteria can be used as antibacterial against Pseudomonas aeruginosa. This research aims to determine antibacterial activity of secondary metabolites purwoceng root endophyte bacteria against P. aeruginosa growth, characterize the group of secondary metabolites produce by endophyte bacteria and identify potential endophyte bacteria using molecular character 16S rRNA gene sequence. Method used in this research such as antibacterial activity assay to determine potency of antibacterial compound produce by endophyte bacteria against P. aeruginosa growth, thin layer chromatography (TLC) assay to characterize the group of antibacterial compound and sequencing of 16S rRNA gene to determine the strain of potential endophyte bacteria. The result of bacterial activity showed GP2, GP14 and DG1 isolate produce antibacterial compounds by forming inhibitory zone in antibacterial activity assay. Antibacterial compounds produce by GP2, GP14 and DG1 isolate belong to alkaloid family which has the ability as antibacterial. Based on molecular identification GP14 and DG1 isolate has the similarity with Bacillus subtilis by 99%, while GP2 isolate has the similarity with Enterococcus faecalis by 99%.

Keywords
endophyte bacteria, purwoceng, antibacterial, 16S rRNA, P. aeruginosa

Topic
Microbiology

Link: https://ifory.id/abstract/uRMpvzPLK76G

Corresponding Author
Maghfiroh Gesty Maharani

Institutions
1) Biology Departement, Faculty Mathematics and Science
Universitas Negeri Malang, St. Semarang 5 Malang, 65145, Indonesia
* srirahayulestari[at]um.ac.id

Abstract
Hypercholesterolemia is the highest risk of CVD which is the biggest disease leading death. One of the Indonesian medicinal plants is single bulb garlic with high flavonoids concentration. Lanosterol synthase, an enzyme on the final stage of cholesterol synthesis are the appropriate inhibition stage for drug. The purpose of this study was to analyze the potential of single bulb garlic flavonoids (quercetin, isoquercetin, and kaempferol) in inhibiting lanosterol synthase. Computational docking analysis was performed using Pyrx, Pymol, Discovery studio, also webserver to predict ADMET and biological activity. Lanosterol synthase was obtained from PDB (PDB ID: IW6J) with RO 48-8071 as native ligand used for control. The results showed binding energy RO 48-8071 (-10,3 kcal / mol), quercetin (-9.8 kcal / mol), isoquercetin (-6.8 kcal / mol ), and kaempferol (-9.9 kcal / mol). Based on interaction and bonding distance, flavonoids have more stable than control. Flavonoids also have potential as APOA1, HMOX1 enhancers, lipid peroxidase inhibitors, cardioprotectant and hepatoprotectant, had high distribution volumes, low toxicity and clearance. This result indicated that quercetin, isoquercetin, and kaempferol from single bulb garlic could be potential ligand to treat hypercholesterolemia, and could proceed to in vitro and in vivo study by improve the absorption.

Keywords
Antihypercholesterolemia, Single Bulb Garlic, Flavonoids, Molecular docking

Topic
Bioinformatics

Link: https://ifory.id/abstract/xJjUPThvgBfM

Corresponding Author
Lusi Suciati

Institutions
a) Biology Departement, Faculty Mathematics and Science
Universitas Negeri Malang, St. Semarang 5 Malang, 65145, Indonesia
*srirahayulestari[at]um.ac.id

Abstract
Cardiovascular disease is closely related to hypercholesterolemia (increased levels of total cholesterol in the blood). One effort to inhibit cholesterol biosynthesis is by inhibiting the enzyme squalene synthase. The inhibition of the enzyme squalene synthase does not interfere with the biosynthesis of other important biological molecules and thus better side effects are expected for this inhibitor. The purpose of this study was to analyze geraniin, corilagin, and elagic acid compounds of rambutan (Nephelium lappaceum L.) peel extract as squalene synthase inhibitors. Docking simulation has been performed using PyRx. Data from software and web tools were analyzed descriptively and compared with lapaquistat, a control drug that was proven to inhibit squalene synthase clinically. The docking results indicate that all ligands bind to squalene synthase active site and it has more stable bonds. Geraniin is the compound that has the lowest binding free energy (-12.2 kcal/mol). ADMET results show that on average 80% of geraniin, corilagin and ellagic acid compounds are absorbed by human digestion, well distributed, and don-t cause liver toxicity. The overall results indicated that the compounds could be potential as candidates for the structure-based drug design and the development of the pharmaceutical agents to treatment of cardiovascular disease.

Keywords
Geraniin, Corilagin, Ellagic acid, Squalene Syntase, Rambutan Peel, in silico

Topic
Bioinformatics

Link: https://ifory.id/abstract/FEPxfmAJ9yYa

Corresponding Author
Siti Imroatul Maslikah

Institutions
Universitas Negeri Malang

Abstract
Breast cancer is the second leading cause of cancer deaths among women. The most common triggers for breast cancer are excessive expression of estrogen-α receptors (ER-α) which play an important role in the growth, development and pathophysiology of the breast, so that ER-α is an attractive drug target. Tamoxifen is one of the Selective Estrogen Receptor Modulators (SERMs) to treat breast cancer patients, but this drug has a detrimental effect on the uterus, so a safe alternative is needed to use herbal ingredients such as Red Betel which is used by Indonesian people as traditional medicine. This study aims to predict the potential of compounds Kaempferitin, β-amyrin, Piperbetol, Piperine and Sesamin Sirih Merah as inhibitors of α-Estrogen Receptors (ER-α) through Molecular docking method, Potency Activity test and ADMET test using several software and web server. The docking results between the five red betel compounds and ER-α were at the same site as the Thamoxifen drug, through alkyl and hydrogen bonds with the affinity value of the red betel compound lower than the control drug. The results showed that red betel active compounds have the potential to be antineoplastic and antioxidant. Red betel active compounds have a good ADMET profile. The conclusions of this study are red betel compounds namely Kaempferitin, β-amyrin, Piperbetol, Piperine and Sesamin as potential candidates for breast anticancer drugs.

Keywords
Breast Cancer, Estrogen-α Receptor (ER-α), Virtual screening

Topic
Bioinformatics

Link: https://ifory.id/abstract/xudtYymBjZ8n

Corresponding Author
Edwin Widodo

Institutions
1) Department of Physiology, Faculty of Medicine, Brawijaya University
*edwin[at]ub.ac.id
2) Department of Microbiology, Faculty of Veterinary Sciences, Brawijaya University

Abstract
Diabetes mellitus is a metabolic disorder that is characterized by chronic hyperglycemia, which is accompanied by damage, dysfunction of some organs and disturbance of the metabolism. In diabetes type II, there is a decrease of the ability of insulin to work in peripheral tissues (insulin resistance) and β cell dysfunction. As a result, the pancreas is unable to produce enough insulin to compensate for insulin resistance. Infection of Staphylococcus aureus in diabetes mellitus may worsen the condition. It can lead to bacteremia as well as increase the occurrence of inflammation. This research was aimed to know the molecular responses of rats (Rattus norvegicus) treated with streptozotocin and S.aureus via CD4 T cells and TGF-β levels. This research was Completely Randomized Design (RAL) experiment, with five groups of treatment. Group 1 was normal rats, Group 2 was bacteremia treatment with infection of S.aureus 10^8 CFU/mL, and three other groups were 45 mg/kg streptozotocin-induced diabetes with different level of S. aureus infection (10^5, 10^6 and 10^7 CFU/mL). Injections of streptozotocin were done intraperitoneally in high single dose (45 mg/kg). Blood glucose levels was measured after 2 days of STZ injection followed by Staphylococcus aureus injection intraperitoneally. The relative levels of CD4 T cells and TGF-β was measured by flowcytometry. The data was analyzed using One Way Analysis of Variance (ANOVA) (α = 0.05). The results showed an average CD4 levels were relatively experienced 6.9%, 7.9% and 3.5% increase in group 3, 4 and 5 respectively. The average relative levels of TGF-β were decreased 0.7%, 3.1% and 5.1% in group 3, 4 and 5 respectively. In conclusion, the relative levels of CD4 increased while the relative levels of TGF-β decreased in streptozotocin-induced diabetes rats infected with Staphylococcus aureus.

Keywords
CD4, TGF-β, Diabetes mellitus, Staphylococcus aureus

Topic
Biomedical Science

Link: https://ifory.id/abstract/4RULue3nv7AD

Corresponding Author
Miftahul Mushlih

Institutions
1) Faculty of Health Science, Universitas Muhammadiyah Sidoarjo,
Jl. Mojopahit No. 666, Sidoarjo, Jawa Timur.
2) Indonesian Genetic and Biodiversity Community, Jl. Ikan Mujair No. 15, Tunjung Sekar, Lowok Waru, Malang.
3) Faculty of Science and Technology, Universitas Muhammadiyah Sidoarjo,
Jl. Mojopahit No. 666, Sidoarjo, Jawa Timur.
4) Department of Biology faculty of Mathematic and Natural Science, Universitas Negeri Surabaya, East Java Indonesia.

Abstract
Type 2 Diabetes Mellitus (T2DM) is disorder caused by the loss of cell- β insulin secretion or insulin resistance. T2DM occurs by genetic and environmental factor. This study was conducted by analyze the inheritance of polymorphism in T2DM families. The method was used is RAPD-PCR (Random Amplified Polymorphic DNA) using F4 5--GTCTTGGGCA3- primer. Three families whose had a history of type 2 diabetes mellitus used. They are composed by 4 samples as positive control (diagnosed with diabetes mellitus), and 9 samples allele analyzed. 2 sampel from other sources was used as negative control. For the results, there were 7 of 9 samples might positive allele of T2DM.

Keywords
Type-2 Diabetes Mellitus, Polymorphism, RAPD-PCR, Pedigree

Topic
Biomedical Science

Link: https://ifory.id/abstract/EPhWRgFJCtjQ

Corresponding Author
Eko Sri Sulasmi

Institutions
Department of Biology, Faculty of Mathematics and Sciences, Universitas Negeri
Malang, Jl. Semarang 5 Malang 65145, Indonesia

Abstract
Tahura Soerjo Forest Park has an altitude of 1456-1809 masl, temperature 18-22⁰C, humidity 32-77 is a suitable habitat for the epiphytic fern. The study was conducted in June-July 2019 in Tahura Soerjo Forest Park. Exploration includes 17 stations in the Cangar and Watu Ondo regions. The exploration results were Antrophyum reticulatum (Forst.) Kaulf, Athyrium esculentum (Retz) Copel, Belvisia revoluta (Bl.) Copel, Asplenium tenerum Forst. f, Asplenium tenuifolium D. Don, Asplenium decrescens Kunze, Asplenium zimkeranum Kunze, Vittaria elongata Sw, angustata Pyrrosia (Sw.) Ching, Hymenophillum gardneri vdb, Davallia trichumanoides Bl., Nephrolepis cordifolia (L.) Presl, Ctenupteris obliguata (Bl. ) Holtum, Crypsinus enervis (Cav.) Copel, Microsorium zippuli (Bi.) Ching, Monogramma trichoides J. Sm, Phymatodes nigrescens (Bl.) Sm. Morphologically, these epiphytic ferns have characteristics of upright rhizomes, crowded fronds, and live as a colony. Anatomic characteristics of the fronds are homogenous mesophiles, irregular epidermis, secretory trichomes, have kidney-type stomata, whereas in rhizomes have scalariform-type tracheids.

Keywords
Anatomy, Epiphytic, Morphology, Tahura

Topic
Botany

Link: https://ifory.id/abstract/2hjJCFHPyqpr

Corresponding Author
Irmawati Irmawati

Institutions
a) Faculty of Marine Science and Fisheries, Hasanuddin University
Jl. Perintis Kemerdekaan Km.10, Makassar, 90245, Indonesia
* trif.ahwa[at]gmail.com
b) Faculty of Fisheries and Marine Science, IPB University (Bogor Agricultural University), Bogor 16680, Indonesia

Abstract
Asian sebass, barramundi, Lates calcarifer (Perciformes, Latidae) was first described in 1790 by the name of Holocentrus calcarifer by Bloch. The name of genus Lates by Cuvier & Valenciennes (1828) was given to comprise other species, including Nile perch (L. niloticus). Lates calcarifer is a type of fresh-seawater fish that has high economic value comparable to salmon and tuna. The population of Asian seabass in Australia, Myanmar, Sri Lanka and Japan was reported in different types. Lates lacdiva from Sri Lanka had a lower height than L. uwisara (Myanmar) and L. calcarifer (Australia). However, the attempt to describe and assess the diversity of Lates in Indonesia, including in the Bone Bay and Makassar Strait have never been done. This study analyzed the morphology and genetic diversity of barramundi caught in the Bone Bay and Makassar Strait waters as an effort to document and map species richness. Both morphological and genetic diversity of barramundi caught in both sites were low. The body was elongated with black caudal-fin. Barramundi from Bone Bay had a white-silver color, while those from the Makassar Strait were greenish in color. Dorsal-fin was divided in two parts with base of second dorsal fin less than that of first dorsal-fin. Third dorsal-fin spine longest. Dorsal-fin spines of Asian seabass from the Makassar Strait were more varied than that Bone Bay. Anal-fin spines of Asian Seabass from Bone Bay were varied than that Makassar strait with third anal-fin spine longest (III>II>I). The pectoral-fin was shorter than the pelvic-fin. The pelvic-fin did not reach the anus and generally separate, but in some specimens are fused by a thin membrane. Caudal-fin was rounded and consisted of 15-18 weak spines. Body height was 29.30- 33.35% to standard length (SL), with certain specimen was found with height up to 37.50% from SL. One small spine in the operculum with a position above the lateral line, and 4-5 small spines at the bottom of the pre-operculum. The highest genetic distance was 0.16% while the number of haplotypes was two with 0.00045 nucleotide diversity.

Keywords
Asian seabass, barramundi, Lates calcariver, morphological and genetic diversity, Bone Bay, Makassar Strait

Topic
Zoology

Link: https://ifory.id/abstract/HJ4taCQyxjG9

Corresponding Author
Hartatiek Hartatiek

Institutions
1 Department of Physics, Universitas Negeri Malang, Jl. Semarang 5 Malang
2Department of Physics, Universitas Brawijaya, Jl. Veteran Malang

Abstract
The HAp scaffold has poor biodegradation. To increase biodegradation of HAp, it was composited with PVA, PEG and Chitosan polymers which had faster biodegradation properties. Nanofiber PVA/CS/PEG/HAp composites as scaffold in bone tissue engineering are prepared by electrospinning. The composition of HA varies 0, 2, 3, 4, 5 and 6% v. HAp was synthesized from natural deposits and characterized using X-Ray Diffraction (XRD) to obtain phase, crystallinity and crystal size. Surface morphology and porosity of nanofiber composites were characterized by SEM-EDX. Increasing the concentration of HAp can increase the average diameter of the nanofiber and reduce porosity. Composite biodegradation of PVA/CS/PEG/HAp was also evaluated. Biodegradation test was carried out by immersing the sample in SBF solution (Simulated Body Fluid) for 1-4 weeks and the fastest mass reduction occurred in HAp (0%) and decreased with increasing concentration of HAp. This nanofiber HAp/CS/PEG/HAp composite can be applied as a scaffold in bone tissue engineering.

Keywords
morphology, porosity. biodegradation, scaffold, nanofiber

Topic
Biomaterial Science

Link: https://ifory.id/abstract/JwUAx7rPE6ad

Corresponding Author
Andrew Jonatan

Institutions
1 Master Program in Biomedical Science, Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia.
2Department of Orthopaedics and Traumatology, Faculty of Medicine, Universitas Brawijaya, dr. Saiful Anwar General Hospital, Malang, Indonesia.
3Doctoral Program in Medicine, Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia.
4Medical Doctor Study Progam, Faculty of Medicine, Universitas Brawijaya, Indonesia
5Pharmacy Study Program, Faculty of Medicine, Universitas Brawijaya, Indonesia
6 Pathology Anatomy Department, Faculty of Medicine, Universitas Brawijaya, dr. Saiful Anwar General Hospital, Malang, Indonesia

Abstract
In the beginning of the menopause transition, most women experienced an increase in body weight and fat along with a decrease in lean body mass. Weight gain during menopause is related to declined circulating levels of estrogen. Obesity during menopause is related to many diseases, including cardiovascular accident. α7 nAChR is one of the receptors that can be activated by nicotine. Activation of α7nAChR can improve energy homeostasis. Administration of nicotine also can suppress appetite and body weight. Nicotine also has been reported to have antioxidant properties as well. Thus, we conducted this study to explore the effectiveness of nicotine in preventing weight gain and increasing Superoxide Dismutase (SOD) Activity in ovariectomized Wistar rats. We did in vivo true experimental and randomized post-test only controlled group research in 18-20 weeks old Rattus norvegicus. We examine body weight and SOD activity in aorta abdominals as a marker of antioxidant activity. The first group was normal rats (Sham). The second to fifth group underwent bilateral ovariectomy. The second group represents the ovariectomy group (ovariectomy-only/OVX). The third to fifth group was dose 1 (P1 - .0.25 mg/kg), dose 2 (P2 - 0,5 mg/kg), and Dose 3 (P3 - 0,75 mg/kg) treatment group. Group P1-P3 was given daily per-oral nicotine for 28 days, started 3 weeks after ovariectomy. During day 29 after treatment, the organs were harvested. After treatment with nicotine was given, all the nicotine-treated group has lower body weight and weight gain compared to Ovx group. During the last measurement, statistically significant lower body weight was found in group P2 and P3 compared to Ovx group. P3 group was significantly higher SOD activity compared to the OVX group. Finally, we conclude that nicotine administration can prevent weight gain and it has antioxidant manner.

Keywords
Body Weight, Menopause, Nicotine, Obesity, Superoxide Dismutase

Topic
Biochemistry

Link: https://ifory.id/abstract/8r7qBh6ZEYQj

Corresponding Author
Sugeng Maryanto

Institutions
1)Nutrition Study Program, The University of Ngudi Waluyo
2)Pharmacy Study Program, The University of Ngudi Waluyo
Diponegoro street No. 186, East Ungaran, Semarang Regency Central Java 50512, Indonesia
Email: rivamustika86[at]gmail.com

Abstract
Undernutrition in children is one of the health problems in Indonesia. Nutritional therapy for children with under nutrition at the recovery stage can be given Formula Modisco III. Formula Modisco III can be modified with the addition yellow pumpkin (Cucurbita moschata Durch) as local food. This research to determine the value of energy, protein, fat and fiber in the modified modisco III formula with pumpkin. Modification and development of the Modisco III formula with the addition of pumpkin and nutrient analysis test. The result in this study was analyzed of the nutritional value of modified modisco III formula with the addition of yellow pumpkin every 100 g containing protein: 3.17 g, fat: 25.41 g, carbohydrates: 26.25 g, and fiber 8.52 g, from the nutritional value can be converted energy by 346 kcal/100g. The energy value is higher than the original Modisco Formula which only contains 140 kcal /100 g. The energy value of the formula Modisco III with the addition of pumpkin is higher than the standard Modisco formula.

Keywords
Modisco, pumpkin, malnutrition

Topic
Biochemistry

Link: https://ifory.id/abstract/7Rg2WJYyEFdp

Corresponding Author
Dea Ajeng Pravita Suendi

Institutions
a) Graduate School of Biotechnology, Postgraduate Program, University of Jember, Jl. Kalimantan No. 37, 68121, Kampus Tegalboto, Jember, East Java, Indonesia
**deaajeng56[at]gmail.com
b) Department of Biomaterials, Graduate School of Biomedical and Health Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8553, Japan
c) Center for Development of Advanced Sciences and Technology, University of Jember, Jl. Kalimantan No. 37, CDAST UNEJ, Jember 68121, East Java, Indonesia
d) Faculty of Agriculture, University of Jember, Jl. Kalimantan No. 37, Krajan Timur, Sumbersari, Jember 68121, East Java, Indonesia
e) Faculty of Dentistry, University of Jember, Jl. Kalimantan, Krajan Timur, Sumbersari, Jember 68121, East Java, Indonesia
*banun_k.fkg[at]unej.ac.id

Abstract
Cryopreservation of Bone Marrow Stromal Cells (BMSCs) is needed to maintain the biological properties and minimize isolation repeatedly. The aim of this study was to optimize cryoprotectant agents to maintain the proliferative capability of BMSCs. This study used three group of combinations; 1) 90% FBS + 10% DMEM; 2) 90% DMEM + 10% DMSO; 3) 90% FBS + 10% DMSO and Cell Banker 1 (CB1) as a positive control. The study showed cell morphology of spindle, round and flatten-shaped. The cell viability in the second combination was 69.73% and 79.74%, the third combination was 68.08% and 74.85% while CB1 was 81.5% and 85% stored at -80oC and LN2, respectively. Cell density post-thawed in the second combination was 26.5x104 cells/ml and 31.25x104 cells/ml, the third combination was 24x104 cells/ml and 32x104 cells/ml while CB1 33x104 cells/ml and 35.5x104 cells/ml at -80oC and LN2, respectively. Cell proliferation capability differed significantly was (p<0.05). Population doubling time increases as long as with increasing incubation time. The use of the second and third combinations has the ability to recover almost as close to CB1 in post-thawed of cell proliferation capability so that it can be an option for cryopreservation of cells.

Keywords
Bone Marrow Stromal Cells, Cryopreservation, Cryoprotectant Agents

Topic
Biomedical Science

Link: https://ifory.id/abstract/p3zcmHGedJny

Corresponding Author
Ari Priharta

Institutions
Universitas Negeri Malang
STUST, Taiwan

Abstract
The wearable devices are gaining its popularity in recent days. This paper presents the performances of our smart shoes as a wearable biomedical device in capturing the heart beat signal from dorsalis pedis. In common, heart beat are measured from artery that located on chest, hand, fingertip and ear or neck. Even though the pulse from dorsalis pedis has a smaller amplitude compare to the other artery location, the integrated sensor on our smart shoes can capture the signal wih high precision. The experiment had been conducted for several times to 3 groups of age of the healthy male and female, there are kid, mature, and elder. The obtain results show that the smart shoes sensor could measure the the dorsalis pedis pulse accurately. Compare with the common measurement and other commercial devices, our device gives a small gap in rms error, which is acceptable for clinical need.

Keywords
accuracy, dorsalis pedis, heart beat, smart shoes, wearable device

Topic
Biomedical Science

Link: https://ifory.id/abstract/NKB8P2HdE7CR