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Corresponding Author
Asri Lestarini
Institutions
(a) Faculty of Medicine and Health Sciences, Warmadewa University
Jalan Terompong no 24 Denpasar, 80235, Indonesia
*asrilestarini[at]gmail.com
(b) Tabanan Hospital, Bali, Indonesia
(c) Sanjiwani Hospital, Bali, Indonesia
(d) Faculty of Medicine, Gadjah Mada University, Indonesia
Abstract
Diabetic nephropathy is one of the complications caused by metabolic disease which could lead to renal failure. A marker that can potentially be used for early detection of diabetic nephropathy is urinary albumin-creatinine ratio (uACR). This study aimed to measure correlation between uACR and the stage of renal failure. Crosssectional study was conducted in March until August 2018. Participants were clinically examined, and blood and urine were taken. the stages of renal failure was determined by estimated Glomerular Filtration Rate (eGFR). Data was statistically analyzed by One way ANOVA and Pearson correlation. Of 107 participants, 79 participants were eligible for inclusion in data analysis. The participants were grouped into 5 stages of renal failure. Urinary albumin-creatinine ratios in stage 1,2,3,4,5 were 204.9, 125.9, 474.9, 604.7 and 2895.6 respectively. Posthoc analysis showed significant differences in uACR between stage 5 and stage 1,2,3 respectively (p<0.05). Significant correlations were found in uACR with SC (r 0.354, p 0.001), BUN (r 0.244, p 0.032) and eGFR (r -0.345, p 0.02) respectively. Urinary albumin-creatinine ratio had positive correlation with serum creatinine and blood urea nitrogen, while had a negative correlation with estimated glomerullar filtration rate
Keywords
renal failure, urinary albumin-creatinine ratio
Topic
Biology
Corresponding Author
Lida Amalia
Institutions
Institut Pendidikan Indonesia Garut
Abstract
Javanese Edelweis (Anaphalis javanica) is a pioneer plant that has enormous ecological benefits. This plant is living on volcanic ash deposits and crater soils as on Mount Papandayan. The presence of Javanese Edelweis are endangered. This study aims to: (1) compare the density of Javanese Edelweis on Mount Papandayan especially in Tegal Alun, Tegal Bungbrun and Pondok Saladah; (2) knowing the effect of abiotic factors (temperature, light intensity and soil pH) on the density of Javanese Edelweis. The method used in this research is quantitative descriptive research method. Data collection was carried out using the 100 meter belt transect method with a width of 5 meters in three sample locations: Tegal Alun (28 transects), Tegal Bungbrun (3 transects) and Pondok Saladah (10 transects). The results indicate that there is a difference in the density of Javanese Edelweis between TegalAlun, Tegal Bungbrun and Pondok Saladah. The density value in Tegal Alun is 2275.7 trees/hectare, Tegal Bungbrun 946.7 trees/hectare and Pondok Saladah 366 trees/hectare. Whereas for abiotic factors it is known that air temperature, light intensity and soil pH simultaneously influence the density of Javanese Edelweis with R value 85% and influence 70%.
Keywords
Anaphalis javanica, Javanese Edelweis, abiotic factors, density.
Topic
Biology
Corresponding Author
Vallery Athalia Priyanka
Institutions
a) Department of Biology, Faculty of Mathematics and Science Nature, Universitas Indonesia, Jl. Prof. Dr. Sudjono D Pusponegoro, Depok 16424, Indonesia
*vallery.athalia[at]ui.ac.id
b) Departement of Food Technology, Faculty of Agricultural Technology, Widya Mandala Catholic University Surabaya, Jl Dinoyo 42-44, Surabaya 60625, Indonesia
c) Departement of Biology, Faculty of Biotechnology, Atma Jaya University Yogyakarta, Jl. Babarsari no 44, Sleman 55281, Indonesia
Abstract
Meat-based food products are at risk for other meat mixtures that are difficult to detect with the naked eye. The advancement of molecular technology offers the convenience of detecting contaminants in food products. The purpose of this study was to detect pork contamination in beef sausage from several markets in Yogyakarta City using PCR and sequencing, and knowing effectiveness and sensitivity primers. The study using 6 primers, consisting of 2 universal mammals and 4 specific pigs. Samples used were 9 sausages. Seven samples from Yogyakarta, 2 samples from Thailand. Positive samples of mammalian DNA with universal primary mammals namely P195 and CB by 100 % and 0 %. CB primers amplify mammalian DNA in all samples with no specificity. Percentage of pig DNA with P14, PPA6, PPA8 and pork specific primers were 88.89 %, 22.22 %, 22.22 % and 22.22 % respectively. PPA6, PPA8, and pork amplify pig DNA are not specific, whereas P14 is specific, which tested for sensitivity. Positive pig sample was made by varying concentrations of 1, 2, 5, 10, 15, 20, 25, and 100 %. P14 can detecting up to 1 %. Positive pig sample were taken for sequencing. Sequencing shows Sus scrofa was detected.
Keywords
BLAST; primer P14; sequencing; Sus scrofa
Topic
Biology
Corresponding Author
Dewa Ayu Putri Sri Masyeni
Institutions
Faculty of Medicine and Health Sciences, University of Warmadewa
Abstract
In developing countries the work-related Musculoskeletal Disorders (MSDs) is mostly occurs. The research of Indonesian Health Department in 2005 was found out most MSDs was due to worked-related disorders in their job. The aims of this study was to find out the differences of MSDs between the taxi drivers and taxi bike in Kuta District. The design of this study was cross sectional which was analyzed. There was 60 subjects were involved in this study which age ranged 25-50 years old and they had experiences more than one year. The MSDs was measured by Nordic Body Map quitionnaire with modified Likert scale. Data was analyzed by SPSS 23. The result were showed as follow: the average of MSDs of taxi drivers and taxi bike were 43,30±6,23 and 46,60±5,30, respectively. The difference of both above was significant difference (p<0,05). It was concluded that there was significant difference between MSDs of taxi drivers and taxi bike.
Keywords
Taxi driver, taxi bike, musculoskeletal disorders, Nordic body map
Topic
Biology
Corresponding Author
I Gede Suranaya Pandit
Institutions
(a,*)Faculty of Agriculture, Universitas Warmadewa, Denpasar, Indonesia
suranaya_pandit[at]yahoo.com
(b)Post Graduate Program, Universitas Warmadewa, Denpasar, Indonesia
(c) Faculty of Medicine and Health Science, Universitas Warmadewa, Denpasar, Indonesia
Abstract
Tilapia fish that is boiled with Balinese spices or it is also called by Balinese people as nila nyat nyat, exactly is one of the most popular Balinese dishes because it is very tasteful. But until now, none has done packaging and storing it at cold temperature for resale. The aim of this study is to understand the interaction among the differences in type of packaging and storage time on quality of tilapia fish that is boiled with Balinese spices. We used factorial completely random design with four replications, first factor is type of packaging, that is consisted of 2 levels: A1=Styrofoam box and A2=Plastic box, second factor is storage time, that is consisted of 3 levels: B1=0 day, B2=3 days, B3=6 days. Objective observation includes water content, protein content, total volatile bases, total plate count, whereas subjective observation or organoleptic such as appearance, texture, odour, and taste. The result of the study showed that there was no interaction between the type of packaging and storage duration on the quality of tilapia fish that is boiled by Balinese spices. Based on objective observation, storage time at cold temperature still maintain good quality of tilapia until sixth day.
Keywords
Type of packaging, storage time, tilapia, nila nyat nyat
Topic
Biology
Corresponding Author
Abdul Syukur
Institutions
*1 Postgraduate Program and Department of Sciences Education,
2 Faculty of Teacher Training and Education, Mataram University, Indonesia;
Jalan Majapahit No 56 Mataram Nusa Tenggra Barat; Phone: +62 370 623873
*12Corresponding author: Abdul Syukur - Email: syukurbiologi[at].unram.ac.id.
Abstract
Mangrove is a plant species that grow in a unique environment and serve as breeding, spawning, hatching, and nursery grounds for many marine species. However, the damage of mangrove has a negative impact on the diversity of fauna associated. This study aims to prove and evaluate mangrove replanting of fauna diversity on the South Coast of East Lombok.. Data collection were done through observation, surveys and quadrant methods The data were further analyzed to detemine Diversity Index (H) and Dominance Index (D). The analysis showed that the mangrove in Jor Bay has the highest index value of fauna diversity, is 2.762 and the lowest is Lungkak that is 2.146. The mangrove other locations such as Tanjung Luar have a value of fauna diversity index of 2,567, Kedome 2, 361 and Ekas Bay 2,457. However, the index value is highest dominance Ekas bay of 0.176 and the lowest was 0.097 Kedome. The conclusion of this study is that mangrove planting has been successful in increasing the diversity of fauna associated with mangroves on the south coast of East Lombok. Therefore, efforts to maintain the results of mangrove revegetation are a very important part of mangrove conservation.
Keywords
Replanting of Mangrove, Diversity of fauna and Maintenance of mangrove
Topic
Biology
Corresponding Author
Asep Rohayat
Institutions
Biology Department, Institut Pendidikan Indonesia
Abstract
Abstract Additives are chemical compounds added to preserve food. There are two types of additives; natural and artificial additives. The durability of wet noodles is relatively lower compared to other staple food. There are various methods to preserve wet noodles. One of which is the addition of natural additives. Natural additives are chosen because they are safer than artificial additives which are more precarious to human health. The natural additives used are carrots because they contain B-carotene compounds functioning as antioxidants that reduce and or deactivate free radical attacks and ROS (Reactive Oxygen Species) on food caused by microorganisms. These processes increase the durability of wet noodles. The research aimed to determine the effect of B-carotene content in carrots as a natural additive for wet noodle preservation. The method used was an experiment with B-carotene concentration variations in carrots; 0%, 20%, 40%, 60%, and 80%. From the results, it was found that at the 60% concentration of B-carotene, the durability of wet noodles reached the maximum 4 day. This fact showed that the addition of high concentration B-carotene will affect the durability of wet noodles. And carrots can be used as natural additives in preserving wet noodles.
Keywords
B-carotene, Additives, durability, wet noodles
Topic
Biology
Corresponding Author
Chevi Ardiana
Institutions
Biology Department, Institut Pendidikan Indonesia
Abstract
Abstract Bintaro fruit contains a lot of organic compounds that cause mortality of a number of insects including cockroaches. The reasearch aims to determine the effect of various concentrations of bintaro fruit (Cerberra manghas) as bioinsecticides, and to know the effective concentration to achieve mortality from Periplaneta americana cockroaches . The method used in this study is a pure experimental method that uses Completely Randomized Design (CRD) with 5 treatments and 5 repetitions, with different concentrations namely concentrations of 0%, 10%, 20%, 30%, and 40%, so that the sum of all a sample of 125 Periplaneta americana cockroaches. Based on the results of statistical tests using the ANOVA test and Scheffe further test, the treatment of the concentration of bintaro fruit extract (Cerberra manghas) 40% significantly affected the Periplaneta americana cockroach compared with other treatments and in terms of LC50 values, is the mortality rate had reached 50% in observations 5 x 4 hours and more mortality than other concentrations.
Keywords
Bintaro Fruit Extract (Cerbera manghas), Cockroach Mortality (Periplaneta americana)
Topic
Biology
Corresponding Author
Nasrul Rofiah Hidayati
Institutions
Chemical Engineering Study Program, Faculty of Engineering, Universitas PGRI MADIUN
Abstract
ABSTRACT At present the public demand for fuel oil (BBM) is increasing inversely proportional to its availability. One of the efforts to reduce peoples dependence on BBM is to utilize alternative vegetable-based energy such as bioethanol. Bioethanol is a fermented product that can be made from a substrate containing carbohydrates. Breadfruit (Artocarpus artilis) is one of the agricultural products that has a fairly high starch content of 89% and is not included as a staple food source in Indonesia. Utilization of breadfruit is still not optimal because breadfruit is only used as a snack or made into flour. This study aims to make bioethanol with breadfruit raw material (Artocarpus artilis). The method of this study consisted of two stages, the first step was hydrolysis of breadfruit flour with a hydrochloric acid (HCL) catalyst. The concentrations of HCL used in this study were 1%, 1.5%, and 2%. The second stage of fermentation using Saccharomyces cerevisiae with 72 hours fermentation time, 120 hours and 168 hours. From the results of the research it was found that the highest levels of bioethanol to the treatment of HCL 2% concentration and fermentation duration of 168 hours was 17.6%.
Keywords
bioethanol, breadfruit, HCL concentration
Topic
Biology
Corresponding Author
leni sri mulyani
Institutions
Biology Department, Institut Pendidikan Indonesia
Abstract
Abstract Cheese is commonly derived from cows milk by separating whey and curds through a fermentation process, involving both aerobic and anaerobic microbial activities. Cheese making is done through a process called milk coagulation with the help of rennet. However, rennet is not easily obtained and rather expensive. Fortunately, rennet can be substituted by cermai fruit extract in this milk coagulation process. The present study aims at determining the concentration of cermai fruit extract thatcan optimally speed up the clumping process of FH cattle milk in cheese making. The extracts were obtained through smooth blending, filtering, and squeezing. Five experiments were conducted using extracts with different concentrations: 50%, 60%, 70%, 80%, and 90%. The results show that 90% is the most optimum concentration of cermai fruit to help the coagulation process in cheese making. The milk coagulated in the average time of 5.4 minutes. To conclude, cermai fruit extract could determine the milk coagulation process time period and can be used as a substitute for rennet
Keywords
concentration, cermai fruit extract, cows milk.
Topic
Biology
Corresponding Author
Pri Riznaya
Institutions
Universitas Padjadjaran
Abstract
The purpose of this research was to determine the effect of energy and protein balances on the digestive value of female garut lamb. The variables are TDN (Total Digestible Nutrien) value, Crude Fiber (CF) digestive value and Crude Protein (CP) digestive value. The treatments given included R1 (12% protein and 60% TDN), R2 (12% protein and 65% TDN), R3 (14% protein and 60% TDN), R4 (14% protein and 65% TDN), R5 (16% protein and 60% TDN), and R6 (16% protein and 65% TDN). The feed are arrange using field grass, fine bran, onggok, soy sauce pulp, coconut cake, and premix. The design of the room uses a Completely Randomized Design (CRD), with 4 (four) replications. Data analysis used variance analysis and Duncans multiple analysis. The results showed that various protein and energy ratio had significant effect on TDN (Total Digestible Nutrien) value, Crude Fiber (CF) digestive value and Crude Protein (CP) digestive value.
Keywords
: Garut lamb, protein and energy balances, digestive value
Topic
Biology
Corresponding Author
Pri Riznaya
Institutions
Universitas Padjadjaran
Abstract
The purpose of this research was to determine the effect of energy and protein balances on the growth female Garut lamb. The variables are daily consumption, daily weight gain, and conversion ratio value. The treatments given included R1 (12% protein and 60% TDN), R2 (12% protein and 65% TDN), R3 (14% protein and 60% TDN), R4 (14% protein and 65% TDN), R5 (16% protein and 60% TDN), and R6 (16% protein and 65% TDN). The feed are arrange using field grass, fine bran, onggok, soy sauce pulp, coconut cake, and premix. The design of the room uses a Completely Randomized Design (CRD), with 4 (four) replications. Data analysis used variance analysis and Duncans multiple analysis. The results showed that various protein and energy ratio had significant effect on daily consumption (whereas R1, R3, and R5 gave better results), but did not affect the daily weight gain and feed conversion ratio value.
Keywords
rut female lamb, protein and energy balances, growth
Topic
Biology
Corresponding Author
I Made Kawan
Institutions
a) Department of Fisheries, Faculty of Agriculture, Warmadewa University, Denpasar, Bali
*E-mail: imadekawan[at]yahoo.co.id
Abstract
This study was conducted to determine the effect of salinity which can produce the most fecundity and number of shrimp larvae (Macrobrachium rosenbergii de Man). The experiment was designed using 24 units of randomized block design (RBD) using 20-30 g (I) and 40-60 g (II) heavy parent shrimp, with differences in salinity 0 Promil (0/00), 3 Promil (0 / 00), 5 Promil (0/00), and 7 Promil (0/00) which were repeated 3 times. The results showed that differences in salinity of the parent weight group (20-30 g and 40-50 g) had a very significant effect on fecundity and the number of larvae produced. The highest fecundity and production of shrimp larvae were obtained at salinity 5 Promil (0/00) in the parent weight group 40-60 g.
Keywords
Salinity, fecundity, shrimp larvae
Topic
Biology
Corresponding Author
cecep hidayat
Institutions
Agrotechnology Dept, UIN Sunan Gunung Djati Bandung
Abstract
Swimmer crab flour (Protunus pelagicus) which has high Ca content and Arbuscular mycorrhizal fungi (AMF) can be used to overcome acid soil. The aim of this research was to know the interaction effect of swimmer crab flour and AMF on increasing acidic dry soil productivity for the growth and yield of Japanese cucumber plant. The polybag research was conducted at Nagreg Subdistrict from May to July 2018 using Randomized Block Design with two factors and three repilactions. The first factor was swimmer crab flour doses (control, r1= 9 g 5 Kg soil-1, 12 g 5 kg soil-1,15 g 5 kg soil-1 and 18g 5 Kg soil-1) and the second factor was AMF dosage (control, 5 g crop-1 and 10 g crop-1). The results showed that there was an interaction effect of swimmer crab flour and AMF on the root infection degree , there was an independent effect of swimmer crab flour on flowering time, dry weight of fruits, and harvest index of cucumber, and there was an independent effect of AMF on the harvest index.
Keywords
acid soil, swimmer crab flour, AMF, yield, japanese cucumber
Topic
Biology
Corresponding Author
Budy Frasetya
Institutions
a) Agrotechnology Department, UIN Sunan Gunung Djati Bandung Jalan A. H. Nasution No. 105 Bandung 40614, West Java, Indonesia
*budyftq1682[at]gmail.com
Abstract
The application of salt fertilizer to increase the productivity of lettuce in long term application will decline soil fertility. Implementation of totally organic farming in some region cannot increase productivity rapidly. Combine application of vermicompost with salt fertilizer is a solution to answer decline lettuce production on the first time implementation of totally organic farming. This research aims to study the dose application of vermicompost on the growth of lettuce plant. The research conducted at Cisurupan-Garut West Java Province with altitude 1183 m above sea level from January to March 2018. The method used in this research is randomize block design with seven treatments (A= without vermicompost, B= 5, C= 10, D= 15, E= 20, F=25, G= 30 t ha-1), and four replication. The growth parameter observes in this research is plant height, number of leaves, fresh plant weight, plant dry weight, and shoot-root ratio. The data were collected from the observed parameter than analyzed with variance analyze at five percent signification level. The result of this research shows that the application of 5 t ha-1 vermicompost improves plant growth significantly.
Keywords
Vermicompost, totally organic farming, soil fertility
Topic
Biology
Corresponding Author
Ni Luh Putu Eka Kartika Sari
Institutions
a,b,c,d,e) Faculty of Medicine and Health Sciences, Warmadewa University
Abstract
Noise-induced hearing loss (NIHL) is the common occupational disease especially for fishermen who using boat for fishing. NIHL is a permanent hearing abnormality caused by prolonged exposure to loud noise and the first symptom is normally the inability to hear high-pitched sounds. The Regulation of Indonesian Ministry of Health 2016, mentioned the safe range of noise maximum is 88 dB with the maximum time of exposure is 4 hours. Meanwhile, the noise caused by boat engine is 77,64-97,8 dB. The objective of this study was to assess the risk factor of boat engine noise towards NIHL in fishermen. Audiometric examination was done to evaluate the samples with normal baseline, 25 dB. The study sample comprised 70 volunteers which devided within 2 groups (fisherman and 35 local resident-non fisherman). This study was conducted in Kedonganan Village, Bali. The study results showed that 77% (27 of 35) of fisherman is diagnosed as NIHL with working hour average ±4,86 hours and ±16 years period of exposure. Group with non fishermen showed 2 NIHL condition eithout any exposure from boat-engine (p<0.05). It is concluded that the the noise from boat engine can cause hearing loss. The fisherman have to wear earplug or earmuff or headset to reduce the risk of noise induced hearing loss.
Keywords
NIHL, noise, fishermen
Topic
Biology
Corresponding Author
Budy Frasetya
Institutions
a) Agrotechnology Department, UIN Sunan Gunung Djati Bandung, Jalan A.H. Nasution No. 105 Bandung 40614, Indonesia
*)budyftq1682[at]gmail.com
Abstract
The drip Hydroponics system has a weakness which at a high evapotranspiration condition cant fulfill plant water requirement properly. Improve the composition of plant growth media with cocopeat and rice husk charcoal as a solution to increase Kailan Plant Growth. This research aims to study the effect of a ratio between cocopeat and rice husk charcoal as plant media on the growth of Kailan Plant with drip hydroponic system. The research was conducted at Jatinangor-Sumedang-West Jawa from November to December 2017. The method of this research was use completely randomized design with five treatment (A=Cocopeat 100%; B=Cocopeat 75%+ Rice Husk Charcoal 25%; C= Cocopeat 50% + Rice Husk Charcoal 50%; D= Cocopeat 25% + Rice Husk Charcoal 75%; E= Rice Husk Charcoal 100%), and five replication. The growth indicator in this research is plant height, number of leaves, leaf area, fresh plant weight, and shoot-root ratio. The ratio of cocopeat and rice husk charcoal as plant growth media affect the plant growth of kailan. The result showed that composition grows media Cocopeat 75% + Rice husk charcoal 25% affect plant height, fresh plant weight, and shoot-root ratio.
Keywords
Drip hydroponic system, Composition plant grow media, Cocopeat, Rice husk charcoal
Topic
Biology
Corresponding Author
Yohanes Parlindungan Situmeang
Institutions
a) Agriculture Faculty, Warmadewa University.
Jl. Terompong 24 Tanjung Bungkak, Denpasar-Bali, 80235, Indonesia
*ypsitumeang63[at]gmail.com
Abstract
This study aims to determine the effect of compost and biochar and their interactions on the growth and yield of red chili plants. This study used a randomized block design (RBD) with a factorial pattern, which consisted of two factors: compost (0, 10, 20, and 30 tons ha-1) and the type of biochar (bamboo and coconut shell). The results showed that the interaction between compost and biochar did not have a significant effect on all observed variables. The dosage of compost has significant effect on fruit length, fresh fruit weight, and fresh weight of roots. The biochar treatment has no significant effect on all observed variables except for the number of fruits that have a significant effect. The treatment at different dosage levels was not significant, but the compost dose of 30 tons ha-1 tended to produce the highest fresh fruit weight 185.35 g which increased by 20.94% compared to the lowest yield without compost 153.26 g. The treatment of this type of biochar only had a significant effect on the number of chili fruit, namely coconut shell biochar which gave the highest value of 17.17 chilies and was significantly different from bamboo-based biochar which produced 15.00 chilies.
Keywords
compost, biochar, red chili
Topic
Biology
Corresponding Author
RIZWAR No last name
Institutions
UNIVERSITAS BENGKULU
Abstract
One of the conservation efforts of the Lepidochelys olivacea turtle in Indonesia is by saving its eggs on the beach, hatching, rearing and releasing hatchlings into the sea. However, a lack of knowledge in management has affected many turtle eggs that do not hatch and hatchlings that die during maintenance. Breeding activities are controlled incubation and hatching of eggs in order to make L. olivacea more developed and avoid extinction. Eggs are planted at a certain depth of the nest but it is not known at what depth the eggs have good hatchability. The aim of this study was to determine the hatchability of the eggs and morphometric of the hatchlings (Lepidochelys olivacea) based on the depth of the brood nest. This research was conducted in 2016 at Muara Tembulih, West Coast of Lampung Province. The treatment was designed with a Completely Randomized Design in the form of four (4) treatment of egg incubation depth and 6 replications. The results of the study were treatment with nest depth of 40-45 cm, hatchability (70-73%) and morphometric size of the newly hatched turtle hatchlings (body weight ± 23 g; body length ± 7 cm, carapace length ± 4 cm and width carapace ± 3.52 cm) statistically, much higher than in nest depths of 35 and 50 cm.
Keywords
Keywords: Egg hatchability, hatchling morphometric, Lepidophelys olivaceae, nest depth
Topic
Biology
Corresponding Author
Zulkarnain Zulkarnain
Institutions
Faculty of Agriculture University of Jambi
Jl. Raya Jambi Muara Bulian KM 15, Mendalo Indah, Jambi, Indonesia
*Email: dr.zulkarnain[at]yahoo.com
Abstract
The objective of this study was to develop an efficient protocol for sterilization of sprout explants in tissue culture of oil palm. Plant materials used were plumulae and radicle from sprouts of the D x P Sriwijaya variety obtained from Seed Processing Unit PT Binasawit Makmur, Palembang. The medium used was MS composition was supplemented with vitamins, myo-inositol and sucrose, and the pH was set at 5.8 pmfrac{-bpmsqrt{b^2-4ac}}{2a} 0.02 before being solidified with agar. Cultures were maintained under a light intensity of 1,500 lux and 16-hour photoperiod and temperature of 24 hingga 26 oC. Five protocols for the eradication of explant contamination were tested. i.e. There were 5 (five) different methods of explant sterilization employed, ie: A) explants were washed with sterile aquadest followed by soaking in 70% alcohol for 1 minute; B) explants were washed with sterile aquadest followed by soaking in 1% Benlox 50WP solution for 30 minutes, 1% Agrept 20WP for 30 minutes, and soaking in 70% alcohol for 5 minutes; C) explants were washed with sterile water plus detergent, followed by soaking in 1% Benlox 50WP plus few drops of Tween-80 for 30 minutes, soaking in 1% Agrept 20WP plus few drops of Tween-80 for 30 minutes, soaking in 70% alcohol for 5 minutes; D) explants were washed with sterile water plus detergent, followed by soaking in 0.1% HgCl2 solution for 30 minutes; and E) explants were washed with sterile water plus detergent, followed by soaking in 0.2% Dithane M-45 plus few drops of Tween-80 for 30 minutes, soaking in 1% NaOCl for 5 minutes, soaking in 0.1% HgCl2 plus few drops of Tween-80 for 30 minutes. The results showed that treating the explants with 0.1% HgCl2 for 30 minutes following 0.2% Dithane M-45 and 1% NaOCl applications was proven to be effective for the eradication of contamination.
Keywords
oleaginous plant, in vitro culture, micropropagation, plant propagation
Topic
Biology
Corresponding Author
Budy Frasetya
Institutions
a) Agrotechnology Department, Faculty of Science and Technology, UIN Sunan Gunung Djati, Jalan A. H. Nasution 105, Bandung 40614, Indonesia
*)budyftq1682[at]gmail.com
Abstract
Lettuce is leaf vegetable which consumes as a fresh vegetable. The quality is a crucial role in increasing the market price of lettuce. A large number of nutrient solution is sometimes confusing the farmer or grower which will they choose. The research objectives evaluate five nutrient formulation from local formulator and global formulator.. This reseach conduted in June to July 2018 at Greenhouse of Universitas Padjadjaran, Jatinangor. The experimental design used in this research was completely randomized design with five treatments of nutrient formulation e.g., Sutiyoso (local formulator), Hoagland & Snyder, Hoagland, Shive & Robbins, and Resh Lettuce Anguila. The parameters of lettuce growth (plant height, leaves a number, leaf area, fresh weight of the plant, dry weight of the plant) observe and measure. The data of lettuce plant growth than analyzed with variance analysis &
Keywords
tropic hydroponics, electrical conductivity, lettuce raft system, local formulation
Topic
Biology
Corresponding Author
USMAN KRIS JOKO SUHARJO
Institutions
(a) College of Agriculture, University of Bengkulu,
Jl. W.R. Supratman, Bengkulu 38371.
*) usman_maine[at]yahoo.com,
(b) Department of Agronomy, Colkege of Agriculture, Bengkulu University, Jl. W.R. Supratman, Bengkulu 38371
Abstract
Lots of duku (Lansium domesticum Corr.) Rupit trees have died in the past ten years. However, no high yielding seedling is available for replanting. In this respect, in vitro propagation offers a perfect solution because it allows growers to get millions of pathogen-free and uniform seedlings at a very short of time for rootstocks. The objective of thi experiment was to have the best combination of 2,4-D and BAP in inducing the growth of duku explants. The experiment was conducted from February to June 2018 at the Tissue Culture zlaboratory of the Department of Agronomy and laid in Completely Randomized Design. Seeds of duku were grown in MS media supplemented with 2,4-D (1, 2, 3 ppm) and BAP (2,3,4 ppm). The results showed that all of the media tested produced callous, with different time of formation and qualities. Also, all media produced shoots, but only MS media supplemented with 2 ppm 2,4-D and 2 ppm BAP produced leaves. However, no media produced any root.
Keywords
Duku, BAP, 2,4-D, shoot, in vitro
Topic
Biology
Corresponding Author
Ni Ketut Mardewi
Institutions
Animal Husbandry Departement, Faculty Of Agriculture
Warmadewa University
Abstract
One of the maintenance management on broiler chickens is setting the density number of hens in the cage (cage density). Cage density effect on the comfort of the enclosure in the tropics, temperatures and high humidity can be a major cause of stress in chickens. The space of the cage with a level of density that does not negatively affect the performance of appropriate production and meat quality. This research aims to identify the density of the enclosure temperature and moisture origin as well as its influence on the quality of the meat. A complete Random design are used on research with 3 (three) treatment namely 8, 10 and 12 tail density per m2 of space enclosure. Each treatment was repeated 3 (three times). The results showed the difference in density of cage on maintenance of chicken broiler until the age of 5 weeks, no influence on the quality of the meat. Be advised, with a density of broiler chicken research 12 tails per m2 can be done without affecting the quality of the meat and can save space enclosure used.
Keywords
Broiler Chickens, Meat Quality, Cage Density
Topic
Biology
Corresponding Author
Mirsa Umiyati
Institutions
Universitas Warmadewa
Abstract
Abstract Diarrhea is one of the most common diseases affecting children around the world. According to the Health Service Data of Gianyar Regency, there are 214 diarrhea morbidities per 1000 population with the most diarrhea cases found in Tegalalang District. The role of mothers in giving treatment of diarrhea is important so that children do not fall into worse conditions. Mothers knowledge and behavior is one of the crucial determinants in giving treatment to children suffering from diarrhea. Mothers should know what steps to do first when the child has diarrhea in order to prevent the occurrence of dehydration. This study aims to determine the level of knowledge and behavior of mothers in the early treatment of diarrhea in Tegalalang District Gianyar regency. This research is a descriptive research by using questionnaire instrument which previously has been validated. The samples of this research are mothers who have children suffered from diarrhea six months prior to the research; the number of respondents is 37 which are determined by consecutive sampling. The data were analyzed using univariat analysis test. The results show that mothers with good knowledge level are 22 respondents (59.5%), mother behaviour with enough knowledge in giving treatment are 18 respondents (48.6%), and those who are still lack in providing zinc and how to give good nutrition. The conclusions of this study indicate that mothers with good level knowledge and mothers behavior with enough knowledge and those are still lack of knowledge in some early treatment of diarrhea on children in Tegalalang Gianyar in 2017. Based on this research, the researcher suggests to the related parties specifically to the Puskesmas (Primary Health Care) to conduct counseling on how to give initial treatment of Diarrhea, which can be done at home continuously and sustainably
Keywords
Diarrhea, children, early treatment of diarrhea on children, mothers behaviour, mothers level of knowledge
Topic
Biology
Corresponding Author
Mirsa Umiyati
Institutions
Universitas Warmadewa
Abstract
Abstract Diarrhea is still a health problem in developing countries including Indonesia according to the health department, the problem is evident from the high morbidity and mortality due to diarrhea. The purpose of this study is to describe the level of knowledge, attitudes, and behaviors from head of household about diarrhea in, Banjar Dauh Uma, Bitera Village Gianyar. This research design use descriptive with cross sectional approach, the number of samples are 70 respondents which is selected by simple random sampling. Data that used in this study is primary data that collected by doing an interview and observation directly to the respondents by using questionnaire and checklist as the instruments. The result of this research suggest that the level of knowledge from respondents is classified good category which is 60 respondents (85,7%). Attitudes from the respondents classified as good category which is 48 respondents (68,6%), and the behavior from the respondents classified sufficient category which is 36 respondents (51,4%). Respondents are less aware about prevention of diarrhea such as having clean water and early handling of diarrhea using oralit. As a conclusions, this research showed that the level of knowledge and attitudes of respondents is classified as good category and the behaviors is classified sufficient category. Therefore expected that paramedics can provide information and health promotion to increase public knowledge about diarrhea.
Keywords
Keywords: Knowledge, Attitudes, Behaviors, Diarrhea
Topic
Biology
Corresponding Author
Mirsa Umiyati
Institutions
Universitas Warmadewa
Abstract
Abstract Alcoholic beverages are beverages containing alcohol (C2H5OH) which fermented or distilled from carbohydrates. Alcohol can trigger disorders such as cancer, cardiovascular disease, neuropsychiatry, gastrointestinal tract, infection, injury, and neonatal abnormalities. About 38.3% world population aged 15 years and above consumed alcohol, while in Indonesia 4.6%. A quarter of the consumption (24.8%) is illegal, which is homemade alcohols that are produced or sold out of government control. Therefore, the research was conducted with the aim to know the level of knowledge, attitudes, and behavior of people against alcoholic beverages. This research was designed as a quantitative descriptive research with cross sectional approach. Sampling used was multistage random sampling technique, the number of samples were 96 obtained by the formula. The results of this study indicate that the respondents knowledge level is mostly good (67.7%), response attitude is mostly good (63.5%), and the behavior of alcoholic drinkers is more than half (56,1%). Active role of health workers is needed to provide information on alcoholic beverages and the government needs to provide oversight and firm action in the circulation of alcoholic beverages.
Keywords
Keywords: Alcohol, Alcoholic Beverages, Level of Knowledge, Attitude, Behavior.
Topic
Biology
Corresponding Author
Ratna Komala
Institutions
a) Department of Biology , Faculty of Mathematics and Natural Science, Universitas Negeri Jakarta , Indonesia
b) Student of Magister Degree, Departement Biology, Bogor Agriculture University, Bogor Indonesia
Abstract
Papilio memnon memnon is one of the butterflies groups of the Papilio clan in West Java, Indonesia. This study aims were to determine the morphology, behavior, and life cycle of the P. m memnon butterfly. The research method used is descriptive with survey techniques and literature studies. This study aims to determine the morphology, behavior, and life cycle of the P. m memnon butterfly. The research method used was descriptive with survey techniques and literature studies. Data about morphology, duration of life, and behavior at each development phase during the P. m memnon butterfly life cycle were analyzed descriptively based on direct observation. The results showed the egg phase of P. m. memnon takes about 7-8 days to the phase instar1 of the larvae. The average total time needed in the larval phase is around 23 - 45 days to the pupa phase. The Pre-pupa phase lasts 1-2 days, while the pupa phase lasts 2-3 weeks. P. m. memnon performs the larval phase as many as 5 stages of instar. Food eaten by first phase instar 1 of larvae is the eggshell, which is useful for insect growth and helps eliminate evidence of eggs from parasitic attacks. At each phase of development, the larvae eat the outer skin to obtain additional energy to support growth.
Keywords
Life cycle; larvae; pre-pupae; imago; Papilio memnon
Topic
Biology
Corresponding Author
Tendy Kusmayadi
Institutions
1Faculty of Agriculture, Universitas Garut, Indonesia
2Faculty of Animal Husbandry, Universitas Padjajaran, Indonesia
Corespondence author: Email : tendy84[at]uniga.ac.id
Abstract
This research was about the performance of growth, body weight and milk production of Friesian Holland dairy cow in different agricural ecosystems inIndonesia.The objective of research was to study growth performance data, body weight and FH dairy cow milk production in Indonesia. The research was carried out in the three agroecosystems, Lebakjaya Village (rainfed rice field and dry land/AES DL-Rainfed), Karangpawitan District; Cintanagara village (irrigated rice field and dry lands/AES DL-IRF), Cigedug District; and Pamalayan village (dryland and tropical forest/AES DL-Forest), Bayongbong District.The method of the research was survey method, with the technique of determining farmers proportionally, and sampling farmers and their livestock by random sampling method. The data was processed using SPSS 22.0 for the simple descriptive analysis, and then tested statistically used T-Test.The results it was concluded that growth, body weight and milk yield of dairy cows were influenced by agroecosystems, in the dry land agroecosystems and located on or adjacent to forests (AES DL-Forest), productivity of dairy cows was better than irrigated and dry land with wide balanced rice agroecosystems (DL-IRF) and rainfed and dry land agroecosystems (AES DL-Rainfed).
Keywords
FH Dairy cow, body weight, milk yield, agroecosystem
Topic
Biology
Corresponding Author
Abdul Syukur
Institutions
1)Postgraduate Program of Science Education, Mataram University
2)Department of Sciences Education, Faculty of Teacher Training and Education, Mataram University
12*Correspondent Author: Abdul Syukur, syukurbiologi[at].unram.ac.id.
Abstract
Coastal ecosystems consisting of mangrove ecosystems, seagrass beds and coral reefs have the potential to be developed as ecotourism. Seagrass ecosystems located in the intertidal zone are an integral part of coastal tourism in the area of study. However, its existence has not received serious attention as a component of coastal ecotourism that can contribute to ecological services. This article aims to start an effort towards empowering this potential through the study seagrasses ecology to formulate the potential of ecological services in supporting the development of ecotourism. Data collection were done through observation, survey and questionnaire methods, and data analysis were done descriptively. The analysis showed that the potential of seagrass community tourism services can be grouped into three parts, namely: (1) seagrass community that form seagrass beds in each tourist location, (2) the diversity of species of biota associated with seagrass can be enjoyed by tourists and (3) seagrass community area can be the location of snorkeling at the time of high tide. The conclusion of the study is that the existence of seagrass ecosystems can provide added value from aspects of environmental services in the development of ecotourism in the southern coastal region of Lombok Island.
Keywords
Seagrass ecosystem, Development of coastal ecotourism, Ecologycal services
Topic
Biology
Corresponding Author
Mirsa Umiyati
Institutions
Universitas Warmadewa
Abstract
Abstract Nutritional status is the state of the bodys end result of the balance between the nutrients intake and its utilization. Nutritional problems that usually occur are underweight and overweight. In Indonesia, the prevalence of obesity is 18.8% and underweight is 11.2% which occur in children aged 9-12 years. The prevalence of obesity in children of that age in Bali Province is 20%. Various factors can affect nutritional status such as diet and physical activity. The purposes of this study were to determine the correlation of diet and physical activity with nutritional status in children aged 9-12 years. This study used analytic cross sectional design with 60 samples taken from all students of 4th, 5th and 6th grade of SD Negeri 1 Batannyuh Tabanan that fulfilled the inclusion and exclusion criteria. The variables studied were nutritional status as dependent variable and physical activity as independent variables. The instrument used in this study was a questionnaire that had been tested for validity. Data were analyzed with Somersd correlation test. The results showed that most of the respondents had good diet (51,7%), poor physical activity (53,3%), and normal nutritional status (51,7%). There was a significant correlation with mild positive correlation between diet and nutritional status (p=0,025) and between physical activity and nutritional status (p=0,026). Result of this study are expected to be useful both theoretically and practically in order to perform promotive or preventive actions related to nutritional status.
Keywords
Keywords: Nutritional status, Children, Diet, Physical activity.
Topic
Biology
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