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Corresponding Author
Zico Arman
Institutions
a) Biology Department, 9th Floor Hasyim Ashari Building, Faculty of Mathematics and Natural Sciences, Universitas Negeri Jakarta, Jakarta, Indonesia
b) Universitas Negeri Culture Collection, 9th Floor Hasyim Ashari Building, Faculty of Mathematics and Natural Sciences, Universitas Negeri Jakarta, Jakarta, Indonesia
c) Institute of Bioproduct Development (IBD), Universiti Teknologi Malaysia (UTM), 81130 UTM, Skudai, Malaysia
d) Department of Bioprocess and Polymer Engineering, School of Chemical and Energy Engineering, Faculty of Engineering, Universiti Teknologi Malaysia, 81310, Skudai, Johor, Malaysia
Abstract
Indonesia has many fermented beverages and yeast become one of the agent in fermented process. Yeasts has a role to transform carbohydrate complex into simple compound with release secondary metabolism to environment like amylase enzyme. This study aim to get the isolate of yeast that can potentially produce amylase enzyme. This research conducted in Oktober 2018 until Maret 2019 in Microbiology Laboratory of Universitas Negeri Jakarta. The screening test of potential isolate producing amylase enzyme was perfomed on yeast isolate from eight source indigenous fermented beverages that can grow in YMA medium with pH 2. Screening was carried out on YPSA medium with difussion agar method. From 53 Isolates, there is 16 isolates were able to form clear zone during one day incubation in room temperature there are IB4, IB15, IB20, IB21, IB26, IB36, IL78, IL80, IL81, IL86, IL88, IL97, IL113, IL136, IL146, and IL150, the highest amylolytic index was produce by IL86 (1,019 U/ml). Forming the clear zone is proof that yeast can transform starch become simply sugar like maltose because iodine-starch reaction performed amylose helix and iodine become I3 that filled main core helix, even iodine forms complexes with starch molecules showed a dark purple colour
Keywords
Amylase, Fermented, Yeast
Topic
Biology
Corresponding Author
Yayan Sanjaya
Institutions
UPI
Abstract
Capablity of Hermetia illucens to consume some weeds were conducted. This is an alternative way to reduce weeds in vegetable fields. A descriptive research was conducted by investigating role of BSF on some weeds, ki rinyuh (Chromolaena odorata), Babadotan (Ageratum conyzoides), rumput gajah (Pennisetum purpreum). Each was given BSF larvae with 150 g, 200 gr and 250 gr of weight on 500 gr weed, The result showed that BSF larvae at 50 mg of weight convert weed between 80- 100%, on 100 mg convert 75 % – 97 %, on 150 mg can convert 80 % -100 %. The mortality of BSF larvae on Chromolaena odorata was 90 %, Ageratum conyzoides was 75 %, Pennisetum purpreum was 40%. This showed BSF larvae can be convert weed into BSFlarvae biomass.
Keywords
Hermetia illucens, weeds, weight, bioconvesion
Topic
Biology
Corresponding Author
Fida Rachmadiarti
Institutions
Universitas Negeri Surabaya
Abstract
Ludwigia adcendens and Ludwigia grandiflora are water weeds rapidly grow and disperse. This plant is mainly found in ponds, swamps, and polluted water channels. This means that both plants are capable of absorbing organic and inorganic waste, including heavy metals as Cd (cadmium). The objective of the research to describe capability of L. grandiflora and L. adcendens in Cd Solution at various concentration as individual species or as consortium. These plants were acclimatized first in hydroponic for 14 days, then moved to medium with varying treatments; only L. grandiflora, only L. adcendens and combination of both species at different Cd concentration (0, 5, 10, and 15 ppm). Level of Cd was analyzed at 7th and 14th day using Atomic Absorption Spectrophotometer (AAS), while wet biomass of each group was compared. Data collected was analyzed statistically. Capability of L. grandiflora and L. adcendens in Cd Solution from low to high was found to be L. grandiflora, L. adcendens > consortium of both species, in which bioconcentration factor (BCF) > 1 and translocation factor (TF) < 1. Concentration of Cd was also found to be higher in the roots than in leaves. Colour of leaves also supported these plants as Cd phytoremediator; plants with higher Cd accumulation had yellow leaves compared to plants accumulated lower Cd level.
Keywords
Ludwigia adcendens, Ludwigia grandiflora, BCF, TF, and Cd metal
Topic
Biology
Corresponding Author
Ferdy Semuel Rondonuwu
Institutions
Department of Physics, Faculty of Science and Mathematics, Universitas Kristen Satya Wacana, Jl. Diponegoro 52-60, Salatiga, Indonesia
Abstract
Introduction The study focuses on the light-harvesting function of carotenoids. How carotenoids capture the photon energy and efficiently transfer its singlet energy to the neighboring bacteriochlorophyll in the pigment-protein antenna complexes (LH2) has been studied. The 11Bu- state in the light-harvesting function are critically investigated, tracing the excited-state dynamics of carotenoids free in solution and bound to the LH2. Aim of the study The goal of this study is to reveal the detailed mechanisms of light-harvesting function. The research focuses on the roles of the 11Bu- state in the light-harvesting function of Cars. A series of investigations were carried out to critically examine the roles of the 11Bu- state in the light-harvesting function. Method The internal conversion processes in Cars were investigated by the use of subpicosecond time-resolved absorption spectroscopy for a series of Cars in solution and bound to the pigment-protein antenna complexes. including neurosporene (n = 9), spheroidene (n = 10), lycopene (n = 11), anhydrorhodovibrin (n = 12) and spirilloxanthin (n = 13). The SVD and global fitting were applied to of spectral-data matrices. Results The results lead to a branched relaxation scheme of energy deactivation including (1) the singlet internal conversion in the sequence of 11Bu- 11Bu- > 21Ag- > 11Ag- (ground), and (2) the singlet-to-triplet conversion of 11Bu- > 13Ag followed by triplet internal conversion of 13Ag > 13Bu. The relaxation scheme took place in subpicosecond to decapicosecond time scales. Conclusion The 11Bu- state plays essential roles in light-harvesting mechanisms as a mediator state in the process of internal conversion, an acceptor state in the Car-to-BChl singlet-energy transfer and a precursor state for the rapid formation of the Car triplet state.
Keywords
Carotenoid singlet energy, relaxation scheme, SVD, Light-harvesting
Topic
Biology
Corresponding Author
Ambar Sulianti
Institutions
UIN Sunan Gunung Djati
Abstract
Allergic rhinitis decreases the sufferers productivity so that it affects emotions. This study aims to examine emotional dysregulation in people with allergic rhinitis. This research is a quantitative study by analyzing emotional regulation instruments for 60 productive age allergic rhinitis sufferers. Statistical analysis of Kruskal walls and post hoc Mann Whitney between aspects showed that there were 3 groups of emotional regulation levels. The results showed that patients with allergic rhinitis had low self-control and confidence. Respondents have moderate emotion managing, emotion modify, and concentration levels. There were no difficulties in evaluating emotions in the research subjects. Allergic rhinitis causes an embarrassed person to face other people when sneezing and snot. The condition of sudden sneezing and the urge to itch the nose makes it difficult for respondents to control themselves. However, patients with allergic rhinitis can still concentrate on their work and can manage and modify their emotions.
Keywords
allergic rhinitis, regulation of emotions, confidence, concentration, emotional modification.
Topic
Biology
Corresponding Author
ni putu diah witari
Institutions
a) Faculty of Medicine and Health Sciences, University of Warmadewa, Denpasar, Bali
*) diahwitari[at]gmial.com
b) Mangusada General hospital, Badung, Bali
Abstract
Premature rupture of the membranes is the spontaneous rupture of the membranes when it has not shown signs of labor, or if one hour later there are no early signs of labor. Some risk factors for premature rupture of membranes in the mother can be influenced by spelling factors, maternal age, parity, gestational age and a history of premature rupture of membranes (KPD). The purpose of this study was to determine the characteristics of maternity with premature rupture of membranes at Mangusada General hospital. This research is a descriptive study using secondary data. The results showed that the characteristics of maternity with early rupture of amniotic events in Mangusada General hospital were based on the highest maternal age at the age of 20-35 as many as 175 cases (70.6%), based on the highest age at term (> 34 weeks) by 213 cases (85.9%), based on the most parity occurred in nulliparous as many as 134 cases (54.0%), based on the majority of maternal occupations in private employment as many as 122 cases (49.2%), based on the previous history of KPD the most occurred in mothers without history Previous KPD was 218 cases (87.9%).
Keywords
premature rupture of membranes, Mangusada General hospital
Topic
Biology
Corresponding Author
Mirsa Umiyati
Institutions
Universitas Warmadewa
Abstract
Obesity is a pathological state as due to exceed to consumption of food according the recommended daily food. The effect of the big consumption of food is the incident of obesity in children. The other factor of obesity are less physical activity, genetic factor, and fast food consumption. In Indonesia obtained that the problem of overweight and obesity in children aged 5 to 12 consecutive years of 10.8% and 8.8%, is close to the estimated world figure in 2020. The highest prevalence incidence of obesity in Bali Province is in Badung regency followed by Denpasar and the lowest is in Karangasem regency. The aim of this study was to find out the characteristics of obesity in children aged range 9-12 years in West Denpasar. The research method was cross sectional approach which was carried out among elementary school children in West Denpasar. The characteristics was observed concist of gender, physical activity pattern, and diet. The result were showed as follow the most common obesity in the study was found in male children (57%). The highest percentage (53%) of obesity was at 11 years old which among children who often eat fast food. Obesity was found most often (53%) in children who often eat fast food, followed by children who rarely eat fast food (37%), and at least (10%) in children who never eat fast food. The obesity children who do light activity were 61%, moderate activity were 23 %, and heavy activity were 16% respectively. It was concluded that the highest obesity percentage was due to light physical activity was often eat fast food.
Keywords
children aged range 9-12 years, child characteristics, obesity
Topic
Biology
Corresponding Author
Dewi Jumiarni
Institutions
1) Universitas Bengkulu
2) Universitas Sriwijaya
Abstract
Biodiesel production technology from microalgae is widely considered as a potential and efficient method. This research was conducted to characterize microalgal species isolated from some lowlands in South Sumatera and investigated their potential for biodiesel production. Five microalgal culture isolate were selected and identified as strains of Chlorella sp PKB, Chlorella sp PPP, Chlorella sp SB, Crucigenia quadrata PTA and Scenedesmus sudetica PTA. These isolates were determined the growth rates, biomass productivity and total lipid content. Under similar environmental conditions, Chlorella sp PKB showed the highest lipid content comparison others. The results suggest that Chlorella sp PKB can be a possible candidate species for biodiesel production.
Keywords
Microalgae, biodiesel, lowland
Topic
Biology
Corresponding Author
Komang Trisna Sumadewi
Institutions
a) Faculty of Medicine and Health Science, Warmadewa University
Jalan Terompong no 24, Denpasar 80235, Indonesia
*drtriscel[at]gmail.com
Abstract
Obesity is one of the greatest factors causing health problems. Many studies have shown a significant association between obesity and lung disorders in the form of obstructive sleep apnea (OSA) and asthma. The purpose of this study is to know the ratio of lung function before and after exercise in the normal and obesity group. The research design used was quasi experimental with pretest and posttest design, using Pearson ChiSquare hypothesis test, Wilcoxon Sign Rank Test and Mann Whitney U Test. There are 60 samples divided into normal and obese with an average age of 19.47, consisting of men and women in each of 30 people. The result of this research is in the normal group average of delta value of FEV1 equal to 0,13, FVC equal to 0,10, and lung function equal to 0,87. While in the obese group the mean value of delta FEV1 is -0.17, FVC of 0.00, and lung function of -4.92. The conclusion of this study is the change of lung function between normal and obese group showed a significant difference, that is an increase in the normal group, but in the obese group actually decreased.
Keywords
Obesity, Lung Function, Exercise Induced Bronchospasm (EIB), exercise
Topic
Biology
Corresponding Author
Epa Paujiah
Institutions
a) Department of Biology Education, Faculty of Tarbiyah and Teacher Training, UIN Sunan Gunung Djati Bandung
Jl. Soekarno-Hatta, No. 749, Cimincrang, Gede Bage, Bandung (Kampus II)
b) Department of Biology, Faculty of Sciences and Technology, UIN Sunan Gunung Djati, Bandung
Jl. AH. Nasuiton, No. 105, Cibiru, Bandung
*epapaujiah[at]uinsgd.ac.id
Abstract
Bivalves provide a range of ecosystem services such as turbidity reduction, denitrification induction, and habitat complexity provision. This study aimed to assess the composition and abundance of Bivalves in the intertidal areas of Karang Papak Coastal, West Java, Indonesia as well as analyze its diffeerent based on lunar phase. Three (50-m long) transects with five 1x1-m quadrant plots in each transect line of each research station were established seawards perpendicular to the coast line. The bivalves were composed of one famili (Mytilidae), two genera and two species of which Mya arenaria had the highest density and distribution every time of lunar phase which indicating their ability to adjust with the tide fluctuation every lunar phase conditios.
Keywords
Abundance, Bivalves, Intertidal zone, Karang Papak, Lunar Phase
Topic
Biology
Corresponding Author
Riser Fahdiran
Institutions
(1) Biology Department, Faculty of Mathematics and Natural Sciences, Universitas Indonesia, Depok, Western Java, Indonesia, 16424
(2) Faculty of Mathematics and Sciences, Universitas Negeri Jakarta, Jakarta, Indonesia, 13220
Abstract
Research of composition and diversity of fish species in mangrove ecosystem at Muara Binuangeun, Lebak, Banten, had been conducted at May and November 2015. Catch per Unit of Effort (CPUE) was used as a method with push net and boat net as fishing gear. Fishing was conducted during low tide. Collected samples were preserved with 10 % Formalin Solution and then being identified at laboratory. In total, 106 fishes were collected from 11 families and 28 species. Istigobius ornatus was the most relative abundant species (19,81 %) and the highest relative frequency too. Diversity index value of mangrove ecosystem was 2,846. The composition of collected fish showed several migrant species from seagrass bed and coral reef ecosystem.
Keywords
Fish Composition, Diversity, Mangrove, CPUE, Istigobius ornatus.
Topic
Biology
Corresponding Author
Shofiyatun Nurlaili
Institutions
1Department of Primary Education, Universitas Pendidikan Indonesia, Jl.Dr. Setiabudi 229, Bandung 40154, Indonesia
2Department of Civic Education, Universitas Pendidikan Indonesia, Jl. Dr. Setiabudi 229, Bandung 40154, Indonesia
Abstract
This research was conducted based on the lack of students conceptual understanding of ecology and the environment related to water cycle material. This material is considered important to be analyzed because it relates to life that occurs in the community. The purpose of this study was to give alternative an instructional need for students conceptual understanding of the water cycle that uses a Computer Aided Instruction (CAI) based on learning. The multimedia-based CAI application used by the researcher to understand the concept of the water cycle is implemented on laptops or desktop computers used Windows operating system. The subjects of this research were three students average 11-13 years old. The instruments used were observation, mini-interviews, and tests. The results of this study indicate that understanding students concepts of the water cycle are still lacked and many misconceptions can be seen from the results of student work when drawing water cycles. Furthermore, the use of CAI based learning is needed to help students conceptual understanding of the water cycle material through multiple-choice questions and drawing the water cycle correctly.
Keywords
Computer Aided Instruction, understanding students concepts, water cycles
Topic
Biology
Corresponding Author
Jajang Supriatna
Institutions
a) Study Program of Agrotechnology, Faculty of Agriculture, Universitas Garut, Jl. Raya Samarang No.52A, Garut, Indonesia
*jajangsupriatna[at]uniga.ac.id
b) Indonesian Vegetable Research Institute, Jl. Tangkuban Parahu No. 517, Lembang, Indonesia
Abstract
Morphophysiological characters associated with yield in warm climate area can be used to determine heat stress tolerance characters. Simple correlation and path analysis were used to ascertain the relationship between morphophysiological characters and yield, and to estimate direct effects as well as indirect effects on them. Nine potato cultivars were evaluated in Garut, Indonesia which is situated at an altitude of 732 meters above sea level with an average temperature of 28oC. Randomized Block Design was used in this experiment and it was performed in triplicate. As the result, simple correlation indicated that plant height, leaf number, leaf size, stomata density, dry weight, tuber number, and tuber diameter showed positive significant relationship with yield, whereas chlorophyll content showed negative significant relationship with yield. Futhermore, path analysis indicated that leaf number had the highest positive direct effect on yield followed by plant height, and leaf size, whilst stomata density and dry weight had positive indirect effect on yield through tuber number. In conclusion, leaf number, plant height, and leaf size are the primary character, whereas stomata density and dry weight are the secondary character for selection the heat stress tolerance characters in order to develop high-yield potato cultivars in warm climate area.
Keywords
Correlation; Path Analysis; Heat Stress Tolerance; Potato
Topic
Biology
Corresponding Author
Komang Trisna Sumadewi
Institutions
a) Faculty of Medicine and Health Science, Warmadewa University
Jalan Terompong no 24, Denpasar 80235, Indonesia
*drtriscel[at]gmail.com
Abstract
Flat foot or also known as pes planus is a condition in which the inner or medial arches are reduced or absent so that the sole of the foot will touch the ground. This situation can be physiological which is called a flexible flat foot or pathological which is called a rigid flat foot. Flat foot is one of the most common conditions encountered by pediatrics, which affects around 20% to 30% of the worlds population. With increasing age, the prevalence of flat foot will decrease. Flat foot causes foot instability as a bodybuilder. This study aims to look at the relationship between Body Mass Index (BMI) and the incidence of flat foot in grade 1 and 2 children at SD 5 Sumerta, Denpasar. This study is an analytical study using a cross-sectional approach with a sample of 40 conducted at SD 5 Sumerta Denpasar. The data obtained was carried out a descriptive analysis and correlation test. From this study, it was found that BMI had a correlation with Flat foot (p=0.001; r=0.695). Confidence Interval (CI) 95%. In conclusion, the Body Mass Index has a significant positive correlation with flat foot prevalence.
Keywords
Flat Foot, Body Mass Index, Elementary School Students
Topic
Biology
Corresponding Author
Dewa Ayu Putri Sri Masyeni
Institutions
Faculty of Medicine and Health Sciences, University of Warmadewa
Abstract
The prevalence of cervical cancer in Bali in 2015 was 0.7 ‰ with an estimated number of 1,438 people. The local government targeted "Bali Bebas Kanker Serviks 2020" by held primary prevention and early detection through IVA test, pap smear and HPV vaccinations. Government of Badung supports the program by subsidizing HPV vaccination to female high school students, but there were still many students who not participated because of many factors, i.e. knowledges and attitudes toward cervical cancer. The purposed of this studies were to determine the correlation between knowledges with the attitudes of female high school students in Badung toward cervical cancer and the correlation between attitudes with HPV vaccination participations. The research method used was cross-sectional analytic with samples of 100 female students in SMAN 1 Kuta, SMK Pariwisata Triatma Jaya and SMAN 1 Kuta Utara. Data were collected by personal interviewed using prepared questionnaires and analyzed using Somersd correlation test. Results of the studies showed, that students categorized as moderate knowledges were 65%, students with good attitudes 72%, and 64% of them received HPV vaccinations. There was significant correlation between knowledges and attitudes (p = 0.036, r = 0.214), and no significant correlation between attitudes and HPV vaccination participations (p = 0.207). For the government of Badung, it is hope to continue free HPV vaccination program and for the local health centre to hold counseling about HPV for female senior high school students routinely.
Keywords
cancer, knowledges, attitudes, HPV vaccination.
Topic
Biology
Corresponding Author
Putu Indah Budi Apsari
Institutions
Faculty of medicine and Health Sciences, Warmadewa University
Abstract
Tuberculosis (TB) and malnutrition are the main causes of morbidity and mortality among children in developing countries. Tuberculosis become worse if there are present of malnutrition. In contrary, malnutrition can be caused by chronic inflammatory disease such as TB. This study aimed to analyze the correlation between tuberculosis and nutritional status among children in North Lombok, West Nusa Tenggara, Indonesia. This cross sectional study recruited child TB aged under 18 years as participants. TB was diagnosed by TB scoring in Children, Tuberculin Test, Fine Needle Aspiration Biopsy (FNAB) and Thorax Photos, and nutritional status was assessed using Center for Disease Control and Prevention (CDC) chart. Total 130 children were identified, 65 children with TB and 65 healthy children as control. This study showed that pulmonary TB was present in 72.3% of case, Lymphadenitis TB was 16%, and mixed pulmonary and lymphadenitis TB were 2%. Under-nutrition child was found in 63.1% of cases, malnutrition child was found in 29.2% of cases. There was significant correlation between tuberculosis and malnutrition among children (p<0.05). The highest proportion of TB was in children under five years (52.3%). TB is strongly correlated with malnutrition among children.
Keywords
malnutrition, tuberculosis, children
Topic
Biology
Corresponding Author
Putu Indah Budi Apsari
Institutions
faculty of medicine and health sciences, warmadewa University
Abstract
Cognitive, motoric and language aspect of HIV children is an important issue that affect developmental milestone and quality of life. The effect of HIV infection on cognitive function remains unclear. This study aimed to assess correlation of cognitive scores with CD4 among HIV child in Paediatric polyclinic Sanglah Hospital, Bali. This cross sectional study recruited 68 HIV child age 0-36 months old as participants. Cognitive score was assessed using CAT/CLAMS scores, HIV status was obtained from medical record. The result showed average of CD4 among subject was 29.85 cells/mm3, there was positive correlation between CAT scores with CD4 count (r = 0.33, p = 0.006) and also between CLAMS scores with CD4 count (r = 0.307, p = 0.01). It can be concluded CD4 count correlated with cognitive scores on HIV child.
Keywords
HIV child, cognitive score, CAT/CLAMS
Topic
Biology
Corresponding Author
Putu Indah Budi Apsari
Institutions
FACULTY OF MEDICINE AND HEALTH SCIENCES, WARMADEWA UNIVERSITY
Abstract
Hyperuricemia is a condition of elevated blood uric acid level above normal value. One of the risk factors that causes hyperuricemia is high purine intake. This study aimed to assess the relation between purine intake and blood uric acid level in postmenopausal women in the working area of Puskesmas II East Denpasar. Analytic observational method with cross-sectional approach used 100 postmenopausal women collected by simple random sampling technique. Data of daily purine intake from respondents were obtained by semi-quantitative food frequency questionnaires. The study results showed that daily purine intake of respondents was in the range of 142.48-708.47 mg/day and the average was 408.72 ± 153.50 mg/day. Levels of blood uric acid in respondents were in the range of 2.6-8.4 mg/dL and the average was 5.52 ± 1.661 mg/dL. Purine intake had the strongest effect on blood uric acid level (p = 0,019; OR = 3,198; 95% CI = 1,208-8,461) compared with hypertension and BMI (p = 0,040; OR = 2,573 ; 95% CI = 1.043-6.347; p = 0.378; OR = 1.521; 95% CI = 0.598-3.866, respectively). It can be concluded consume high purin diet cause elevated uric acid level among post menopausal women.
Keywords
Purine, Uric acid, Postmenopause
Topic
Biology
Corresponding Author
Yulilina Retno
Institutions
Universitas Negeri Jakarta
email: yulilinaretno[at]yahoo.co.id
Abstract
The main gynecological problems among women, especially adolescents, are disorders of the menstrual cycle. Menstruation is the decay of the uterine wall which causes bleeding that is influenced by hormones, occurs repeatedly and forms cycles. The menstrual cycle is influenced by hormonal, psychological factors such as anxiety, depression, and stress, and lifestyle such as nutrition and physical activity. This study aims to determine the positive relationship between physical activity and the menstrual cycle in female college students of Biology, Jakarta State University. The study was conducted at the Jakarta State University in October-November 2018. The method used was descriptive method with correlational studies. The number of research samples is 177 female college students. The prerequisite test results show that data were normally distributed and homogeneous. The results of hypothesis testing indicate there is a positive relationship between physical activity and the menstrual cycle, physical activity provides a considerable contribution to the menstrual cycle. The conclusion of this study is that there is a positive relationship between physical activity and the menstrual cycle.
Keywords
college student, menstrual cycle, physical activity
Topic
Biology
Corresponding Author
Ni Luh Putu Eka Kartika Sari
Institutions
(a)Medical Faculty, Warmadewa University, Denpasar Bali
(b)Wangaya Hospital Denpasar, Bali
(c)Eijkman Institute for Molecular Biology, Jakarta
(d)Centre for Disease Control and Prevention, Fort Collins, Colorado
Abstract
Incidence of dengue remains high in tropical and subtropical countries which makes dengue the most common arthropod-borne disease in the region. There is limited data on dengue virus serotype in Bali. This study aimed to assess clinical and virological characteristics of dengue patients in Bali. A cross sectional prospective study was conducted from September 2015 to August 2016. Clinical manifestations were obtained and viral RNA from patient sera were serotyped using conventional RT-PCR. One way Anova was used to compare the clinical manifestation within serotype. Of the 603 patients, 177 cases were confirmed as dengue positive and could be classified into four serotypes. DENV-1, DENV-2, DENV-3, DENV-4. The common clinical manifestations documented were malaise, nausea, anorexia, cephalgia, myalgia, vomiting, athralgia, abdominal pain, retroorbita pain and bleeding. The most prevalent symtoms of cephalgia, bleeding and adominal pain were with DENV-3 infection 63 (66.3%), 11 (11.6%) and 12 (12.6%) respectively. Clinical characteristics such as blood pressure, pulse, temperature, trombocyte, leucocyte, hematocrite and hemoglobin were recorded. Temperature was significantly high in DENV-3 (p 0.030)compared to other serotypes. From all the dengue serotypes that were detected, the most prevalent was DENV-3. There is no significant differences in the clinical manifestations among all serotypes except for high temperature in DENV-3 infection.
Keywords
dengue, serotype, clinical manifestation, Bali
Topic
Biology
Corresponding Author
Yhana Mulyaningsih
Institutions
Faculty of Engineering, Sampoerna University
Jl. Pasar Raya Minggu No 16, Jakarta Selatan, Indonesia
Abstract
Lignin is difficult to be decomposed due to its complicated carbon bonding. Previous studies have discovered that ligninolytic enzymes effectively degrade lignin waste. Numerous microorganism such as white rot fungi have been taken into account to accelerate the decomposition process of lignin. The aim of this study is to detect the capability of the fungi which was isolated from decayed root and stem of oil palm tree. Qualitative test using guaiacol (0.02%) was performed to observe the ligninolytic activity of the fungal isolates. The results of the test indicated that two out of ten fungal isolates were able to produce ligninolytic enzyme. Successful of this study could be used as baseline information of potential fungi which are able to degrade lignin waste in environment.
Keywords
lignin, lignin waste, ligninolytic activity, white rot fungi
Topic
Biology
Corresponding Author
Rini Puspitaningrum
Institutions
1Dept. of Biology, Universitas Negeri Jakarta. Jalan Rawamangun Muka 13220. Jakarta Indonesia
2 Dept of Physics. Universitas Negeri Jakarta. Jalan Rawamangun Muka 13220. Jakarta Indonesia
Abstract
Myoglobin is an intracellular protein which has a vital role in maintaining oxygen hemostasis within living tissue. This protein has the ability to bind and send oxygen to mitochondrial membrane to enable transduction of energy. The liver is the biggest organ in the bodyand has complex functions. Based on the complex functions, liver needs quite a big amount of oxygen, so the metabolism process can be performed well. This study aims to detect mRNA expression in the liver of rats (Rattus novergicus) in normoxia. The study was conducted at the Biochemistry and Molecular Biology Laboratory of the Biology Major of the Faculty of Mathematics and Natural Sciences, UNJ. This study utilized quasi-experiment method. The study results show that myoglobin mRNA is expressed in the liver of rats (Rattus novergicus) in normoxia on 114 pb. This result can create a new paradigm towards myoglobin gene that this gene might be a constitutive gene. This information can be the basis that living tissues have strategies for mechanism of adaptation towards low oxygen environment by increasing the expression of myoglobin in the tissue.
Keywords
myoglobin mRNA, liver, normoxia, Rattus novergicus
Topic
Biology
Corresponding Author
Rini Puspitaningrum
Institutions
1Dept. of Biology, Universitas Negeri Jakarta. Jalan Rawamangun Muka 13220. Jakarta Indonesia
2 Dept of Physics. Universitas Negeri Jakarta. Jalan Rawamangun Muka 13220. Jakarta Indonesia
Abstract
Myoglobin is a hemoprotein with the important roleof being the binding and storage agent of oxygen in tissues. That is why it is important to study the existence of myoglobin in hypoxia tolerant animals, such as the green sea turtle (Chelonia mydas). This study aims to detect myoglobin protein in green sea turtle (Chelonia mydas) organs which consume highly concentrated O2 using spectrophotometry and immunodotblottechniques. In this study, the myoglobin protein of the heart, brain, and liver was analyzed using spectrophotometry at ƛ503nmand immunodot blot techniques with the antibody – anti myoglobin of the green sea turtle (Chelonia mydas). The results obtained is that the myoglobin protein is detected in all organs. The lungs are the organ with the lowest concentration of myoglobin protein from all the test organs. Based on the study results, it can be concluded that genetically, the green sea turtle (Chelonia mydas) would evolve as a tolerant animal toward hypoxia.
Keywords
myoglobin, hypoxia tolerant, green sea turtle (Chelonia mydas), organs which consume highly concentrated O2
Topic
Biology
Corresponding Author
Muktiningsih Nurjayadi
Institutions
(a) Department of Chemistry, Mathematics and Science Faculty, Universitas Negeri Jakarta, Indonesia
(b) Pusat Laboratorium Forensik Badan Reserse Kriminal Kepolisian Republik Indonesia, Indonesia
(c) Balai Besar Uji Standar Karantina Pertanian, Jakarta, Indonesia
Abstract
Salmonella and Escherichia coli are the bacteria the most common cause of food-borne illness. The aim of the study was to detection S. typhimurium and E. coli by Real Time PCR from milk samples. Gene targeted of S. typhimurium was pef and the E. coli gene targeted was fimC. Confirmation test of all primers in contaminated milk with S. typhimurium and E. coli gave different cycle threshold (Ct) value in range 12-13. The detection limit for S. typhimurium with primer pef was in 7,92 pg/&
Keywords
Salmonella typhimurium, Escherichia coli, Milk Sample, Real Time PCR
Topic
Biology
Corresponding Author
Dewa Ayu Putri Sri Masyeni
Institutions
Faculty of Medicine and Health Sciences, University of Warmadewa
Abstract
Sleep is one of the primary needs of the basic requirement for human survival. Sleep disorders can cause some effect in humans. It is estimated that 60-80% of workers with shift work system have impaired sleep quality. This results in a decrease in work productivity and an increase in the occurence of accidents. This study aims to determine the determinants of sleep quality disorders nurses in Tabanan General Hospital. This study used cross sectional analytical observational method by conducting questionnaire based interview. The study population was nurses in Tabanan General Hospital with sampling using consecutive sampling technique with samples of 94 people. The results of this study found that factors that have a statistically significant relationship with the quality of sleep is married / have a marital status (PR = 5,53, IK 95%: 1,50-20,39, p <0,05), nurses with tollders (PR = 3,88, IK 95%: 1,28-11,71, p < 0,05) and work system shift (PR = 19,46, IK 95%: 2,48-152,69, p < 0,05). While the factors that have not statistically significant relationship were sleeping environment (PR = 2,06, IK 95%: 0,72-5,91, p=0,17) and coffee consumption in high quantities (PR = 1,41, IK 95% : 0,53-3,73, p=0,48). Based on the result of research, it is found that marital status, toddler ownership and work system are the determinants of nursing sleep quality disorder in Tabanan General Hospital.
Keywords
sleep quality disorder, determinants, nurses
Topic
Biology
Corresponding Author
Halimatus Sadiyah
Institutions
(a) Biology Education Department, State University of Jakarta, Jalan Rawamangun muka, Jakarta 13220, Indonesia.
*adesuryanda[at]yahoo.com
Abstract
The development of learning media is inseparable from the rapid development of technology. The purpose of this study was to develop a learning media for biology practicum guides in the form of smartphone applications. This research was conducted using the ADDIE model (Analysis, design, development, implementation, and evaluation). The media feasibility test obtained an assessment of 90.28%, material experts 87.75%, biology teachers 91.37% and 88.46% students with very good interpretations. The increase in psychomotor ability test obtained a gain value of 0.49, which means there is an increase in psychomotor abilities of students with a moderate category. The conclusion obtained is that the application of the biology practicum guide has been successfully developed and is suitable for use in learning.
Keywords
smartphone, laboratory manual, learning media.
Topic
Biology
Corresponding Author
ni putu diah witari
Institutions
a) Faculty of Medicine and Health Sciences, University of Warmadewa, Denpasar, Bali
*)diahwitari[at]gmail.com
Abstract
Escherichia coli is a Gram-negative bacterium that has a natural habitat in the human digestive tract. This bacterium besides being a normal flora can also be pathogenic. Escherichia coli is the most common cause of diarrhea. Guava leaves and bay leaves contain essential oils, tannins, flavonoids, and saponins which have antimicrobial effects. The purpose of this study was to determine the difference in the effectiveness of inhibition between ethanol extract of guava leaves and bay leaves on the growth of Escherichia coli bacteria. This type of research is an experimental laboratory. The inhibitory test was carried out by measuring the clear zone around the paper disc using callipers. The results of data analysis using one way ANOVA showed that there were significant differences between groups of extracts with F output = 49.83 and p = 0.00 (p&
Keywords
Escherichia coli, guava leaves, bay leaves
Topic
Biology
Corresponding Author
Dewa Ayu Putri Sri Masyeni
Institutions
Faculty of Medicine and Health Sciences, University of Warmadewa
Abstract
Nowadays, type 2 diabetes mellitus (T2DM) is the most prevalence of diabetes mellitus disease. In Indonesia 10 millions people suffering T2DM which caused Indonesia ranked seventh in the world and predicted to increase 16.2 millions patients on 2040. The use of oral antidiabetic drugs (OAD) like metformin and gliclazide still an option on T2DM therapy. This study aims to determine the differences in the effectiveness of metformin and gliclazide to lower blood glucose in patient T2DM in the sanjiwani gianyar hospital. The variable of this study is oral antidiabetic drugs and blood glucose. The design of this study using cross sectional analitic observational approach with retrospective study. The sample of this study is patient T2DM in internal medicine policlinic of Sanjiwani Gianyar Hospital from Januari-Desember 2016 with 160 subjects who selected through consecutive sampling and fulfil the inclusion and exclusion criteria. This study using secondary data in form of medical record. The data was analyzed by chi square test (α=0.05). The results was showed that the effectiveness of each OAD to decrease blood glucose for 3 months therapy, the used of metformin showed that (70%) 56 sample achieved controlled blood glucose while the used of gliclazide showed that (52.5%) 42 sample achieved controlled blood glucose and there was a significant differences in the effectiveness of metformin and gliclazide with p value is 0.035. The conclusions of this study is metformin more effective than gliclazide to lower blood glucose in T2DM outpatient and advices who gived to hospital, health workers and community.
Keywords
Type 2 diabetes mellitus, Effectiveness, Metformin, Gliclazide, Blood glucose
Topic
Biology
Corresponding Author
erna bagiari
Institutions
Department of Cardiology, Sanjiwani Hospital/ Faculty of Medicine and Health Sciences, University of Warmadewa
Abstract
Abstract The association between lack of thyroid hormones and cardiac dysfunction has been well described. We experienced a case of a 44-year-old woman with dilated cardiomyopathy (DCM) accompanied with long QT secondary to hypothyroidism and hypocalcaemia. Thyroid hormone acts on the myocardium of the heart and peripheral blood vessels. The genomic and non-genomic effects of thyroid hormone are associated with cardiovascular and chemodynamic function. A well documented but mostly overlooked sign in hypothyroidism is the prolongation of the QT interval. Long-QT syndrome, which is characterized by abnormal QT-interval prolongation on the surface ECG and an increased risk of sudden death, usually due to ventricular fibrillation. The mechanism of hypothyroidism on QT prolongation and the occurrence of ventricular tachycardia is unknown and the effect may be idiosyncratic, although coexistent.
Keywords
Delated cardyomyopathy; Long QT syndrome; Total Thyroidectomy
Topic
Biology
Corresponding Author
Riser Fahdiran
Institutions
1) Department of Biology, Faculty of Mathematics and Natural Sciences of Universitas Indonesia, Depok, West Java, Indonesia
2) Division of Reproductive, Endocrinology and Infertility, Department of Obstetrics and Gynaecology, Faculty of Medicine University of Sumatera Utara, Haji Adam Malik General Hospital, Medan, Indonesia
3) Halim Fertility Centre, Stella Maris Women and Children Hospital, Medan, Indonesia
4) Faculty of Mathematics and Science, Universitas Negeri Jakarta, East Jakarta, Indonesia
Abstract
Oocyte maturation rate is an important factor in determining a good fertilization and embryo development rate with in vitro fertilization. Embryos that can develop until blastocyst stage have been proven to have a higher success rate than cleavage stage embryos. The aim of this study is to find out effect of age and body mass index (BMI) in oocyte maturation rate and the successfulness of blastocyst development. In this study, 158 infertile women within age range of 22-47 who underwent IVF-ICSI program in Halim Fertility Center were analyzed. The IVF-ICSI outcomes assessed were number of oocytes retrieved (OR), number of matured oocytes (MO), fertilization rates (FR), cleave rates (CR), the blastocyst rates (BR), and the pregnancy rates (PR). All data were divided into three groups based on WHO BMI classification (normal range, overweight and obese) and four groups based on age classification (up to 30, 31-35, 36-40, below 41). These data were analyzed with student T test method and chi-square. In result, our study concludes that the body mass index shows a negative effect of oocyte maturation process and blastocyst development. However, age had an adverse impact on number of oocyte retrieved, number of matured oocyte, and blastocyst rates.
Keywords
Oocyte Maturation; Blastocyst; Age; Body Mass Index.
Topic
Biology
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