Indonesia Conference Directory

Corresponding Author
Ari Priharta

Institutions
Universitas Negeri Malang
STUST, taiwan

Abstract
A new S2 and S3 sounds detection by using heart rate signal taken from dorsalis pedis artery as a reference is presented in this paper. The aimed of this approach is evaluate the accuracy of the S1 and S2 sounds detection by using dorsalis pedis pulse measurement as reference. The dorsalis pedis pulse were measure by using MAX 30100 sensor controlled by microcontroller, meanwhile the heart sounds were capture by using ECG device. The measurement were taken for 5 minutes for totally 10 male and 10 female subject-s age 20 years. This experiments has been conducted for several times and the average were calculated and compared to the existing solution. The proposed approach gives a small error compare to the common S1 and S2 detection, which mean that the proposed detection method is acceptable as a promising solution.

Keywords
dorsalis pedis, heart beat, heart sounds, S1, S2

Topic
Biomedical Science

Link: https://ifory.id/abstract/chACbP9pU7qr

Corresponding Author
Hendra Susanto

Institutions
1 Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Malang, Malang, Indonesia
2 Department of Physics, Faculty of Mathematics and Natural Sciences, Universitas Negeri Malang, Malang, Indonesia
3 Department of Sports Science, Faculty of Sports Science, Universitas Negeri Malang, Malang, Indonesia
4 Department of Statistics, Faculty of Mathematics, Computation, and Data Science, Institut Teknologi Sepuluh November, Surabaya, Indonesia

Abstract
Metabolic syndrome and physical activity have a strong correlation to the gradual increased of degenerative diseases incidence in the global population. Currently, several approaches have been applied to prevent the progression of metabolic syndrome, including clinical drug therapy dan physical training program. However, limited data were found whether acute medium intensity exercise (AMIE) co-treated with middle rhythm musical exposure can affect to thermogenesis dan fatty acid oxidation. This study was designed by applying AMIE and music on 45 individual, non-professional athletes to evaluate the potential inducer of thermogenic activity (PGC-1α) profile in the muscle and adipose tissue. The preliminary finding of this study shows that AMIE exposure was significantly induced the increase of circulating PGC-1α as linear to the high-intensity exercise group, However, in contrary, the expression of this transcription factor tends to decreased post-AMIE-musical co-treatment. It is suggested that AMIE can induce thermogenesis in the skeletal muscle and adipose tissue by modulating oxidative phosphorylation within mitochondria. In summary, AMIE model may provide an alternative solution to combat metabolic syndrome linked obesity through enhancing the beta-oxidation process. Thus, the expanding investigation with a large sample and long-term monitoring program is required to establish this physical training program in the population.

Keywords
PGC-1alpha, acute medium intensity exercise, music, thermogenesis, metabolic syndrome

Topic
Biomedical Science

Link: https://ifory.id/abstract/TZMdyG7AHLJh

Corresponding Author
Erna Sulistyowati

Institutions
Universitas Islam Malang

Abstract
The combination between herbs and antibiotics might be promising strategy for antibiotic resistance. Abundance studies have been denoted that certain herbs have important roles in bacterial destruction proccess. As an antibacterial, previous study reported that Syzygium polyanthum (bay) leaf extract caused disintegration of bacterial surface and lysis of the bacterial intracellular component. The aim of this study was to determine antibacterial synergistic activity of S. polyanthum leaf extract and chloramphenicol. Staphylococcus aureus was significantly inhibited after treating with high dose of chloramphenicol and low dose of S. polyanthum decoction in disk diffusion test (26 ± 1.52 mm of inhibition zone) in the comparison with low dose of S. polyanthum decoction treatment only (5 ± 4,61 mm of inhibition zone, p <0,05). Similar results were found by combining low dose of chloramphenicol and low dose of methanol extract of S. polyanthum in disk diffusion test (17 ± 0.57 mm of inhibition zone) in the comparison with low dose of methanol extract of S. polyanthum treatment only (7 ± 5,85 mm of inhibition zone). In conclusion, this study suggests that Syzygium polyanthum leaf extract synergizes with antibiotic and has potential as a therapeutic agent for antimicrobial chemotherapy.

Keywords
Syzygium polyanthum, antibacterial, Staphylococcus aureus, synergistic effect

Topic
Biomedical Science

Link: https://ifory.id/abstract/bBfd2XygT7qz

Corresponding Author
Eka Frenty Hadiningsih

Institutions
1) Midwifery Master-s Degree Program Faculty of Medicine, Brawijaya University Malang, East Java, Indonesia
2) Department of Obstetrics and Gynecology, Faculty of Medicine, Universitas Brawijaya, RSUD dr. Saiful Anwar Malang, East Java, Indonesia
3) Department of Clinical Microbiology, Faculty of Medicine, Brawijaya University Malang, East Java, Indonesia
4) Departement of Pathology Anatomy, Faculty of Medicine, Brawijaya University Malang, East Java, Indonesia
5) STIKES Wiyata Husada Samarinda, East Kalimantan, Indonesia
6) Midwifery Study Program, Faculty of Public Health, Indonesian Muslim University Makasar, South Sulawesi, Indonesia

a) Corresponding author: e.frenty.h[at]gmail.com
b) Another author: suryantisudirman[at]yahoo.com, mayasari.ardela[at]gmail

Abstract
Abstract. The Depo medroxyprogesterone acetate (DMPA) is a type of hormonal contraceptive containing progesterone that causes a hypoestrogenic response that inhibits follicle development, prevents ovulation, and causes endometrial atrophy due to suppression of epithelial and stromal cell proliferation. Natural herbs like bengkoang (Pachyrhizus erosus) can be used to reduce the negative effects of DMPA. Bengkoang is widely known as containing isoflavones with chemical properties and functional structures that resemble 17eta estradiol. This study aimed to determine the effect of bengkoang ethanol extract on the number of ovarian follicles, epithelium, and endometrial stroma in DMPA treated Rattus norvegicus. This study used 25 rats as experimental animals, which were divided into five groups consisting of one negative control group (KN) without DMPA and bengkoang extract, and four treatment groups given DMPA at 2.7 mg every three days repeated four times. After the induction period, the hypoestrogenic condition was evaluated through vaginal smear. Three groups were treated by bengkoang extract at a dose of 70 mg/200g BW/day (P1), 140 mg/200 g BW/day (P2), and 280 mg/200 g BW/day (P3). In this study, data were analyzed using a one-way ANOVA and the Least Significant Difference (LSD) test. The results showed that there were significant differences (p< 0.05) in the number of antral follicles, the amount of the epithelium, and the amount of endometrial stroma in the group treated by bengkoang extract dose of 280 mg/200g BW/day. This study suggests that bengkoang extract demonstrated a change in the number of ovarian follicles, epithelium, and endometrial stroma compared to the positive control group treated only with DMPA.

Keywords
DMPA, ovarian follicles, endometrial epithelium, endometrial stroma, Pachyrhizus erosus.

Topic
Biomedical Science

Link: https://ifory.id/abstract/uhbp7Amk6eEw

Corresponding Author
Esha Ardiansyah

Institutions
Biology Department, Faculty of Mathematic and Natural Sciences, Malang State University

Abstract
Dyslipidemia is a disease caused by complications of Type 2 Diabetes Mellitus (T2DM). DMT2 is caused one of them by high-fat foods that can increase the production of Reactive Oxygen Species (ROS) so that it can be prevented and treated with foods that contain lots of antioxidants such as black soybean tempeh and purple sweet potato. This study aims to determine the effect of giving a combination of black soybean tempe extract (Glycine max (L.) merril) and purple sweet potato (Ipomoea. Batatas L) on lipid profile levels (total cholesterol and High Density Lipid levels) in serum blood of the Diabetes Melitus model Type 2 (DMT2). Rats were fed a proximate nutrition 20 g per day and drank 10% sucrose in an ad libitum for 8 weeks and then injected with STZ multiple low dose intraperitoneal (IP), to become diabetic. Rat blood serum was collected and tested by the Spectrophotometry method to determine lipid profile levels after 4 weeks of treatment. The levels of lipid profiles found were analyzed using SPSS. The results of the study showed that the combination of black soybean tempeh extract and purple sweet potato with a ratio of 3: 1 (P3) was the optimal combination to reduce blood sugar levels, total cholesterol, a combination of 2: 2 (P4) treatment was effective to increase blood serum HDL levels in mice DMT2 models.

Keywords
Black Soybean Tempeh, Purple Sweet Potato, Lipid Profile, DMT2

Topic
Biomedical Science

Link: https://ifory.id/abstract/pLFUuCGZhHxX

Corresponding Author
siti rayhanah

Institutions
Universitas negeri malang

Abstract
Propolis is component of honeycomn and has many benefist fot treatmemt the concent of the chemical and its compositiaon are complex, making propolis had various properties, including anticancer or antitumor, antifungial antibiotic and immunomodulator. Tje kidneys is the mains organs that often experience interference due to the toxic effect of commpound, cause the kidneys have a higj blood volume flow and concentrate toxic substance the glomerular filtrate and carry through tubular cell. Purpose of this reserch is to determine the effect of administration propolis extract on necrosis of proxinal tubular epithelial cell of male mice. This research is an expwrimental study of randomize block design with 6 treatmentd . 24 male mice aged 8 weeks was given propolis extract through gastric sonde at dose of 10mg/kg 20mg/kg 30mg/kg 40mg/ kg 50mg/kg and PO given water. After 18days micr in the dislocation and kidney organs mad3 histology preparation with hematoxylin eosin. Each preparation were observed in 2 slide of 4 transversed slice of tje kodney. Each slice observed 3 flieds of view and each field of view is observed in 10 tubule and calculates the nucleus cell thta piknotic karyorexis karyolisis. Analyze the data using a one way anova on the 95% level followed by duncan. the ewsult show propolis extract had a effect to proximal tubular necrosis. The content of tannin and alkaloids propolis is though to have the potential to cause cell membrane damage so that ion transport is disrupted. Resukting in uncontrolled inclusing of ca ions and causing cell death. Doses 10mg/kg began to influence the proximal tubular necrosis mice

Keywords
propolis necrosis proximal tubule

Topic
Biomedical Science

Link: https://ifory.id/abstract/VnLuBFrhbCJZ

Corresponding Author
Esha Ardiansyah

Institutions
1Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Malang, Jl. Semarang 5 Malang 65145, Indonesia

Abstract
Abstract. Diabetic Type 2 is generally characterized by hyperglycemi which causes free radicals in the cell increasing. Excessive free radicals will cause oxidative stress. Conditions of oxidative stress can be seen from the high levels of malondyaldehyde (MDA) as the final product of lipid peroxidation. Free radical activity can be prevented by intake of foods that contain antioxidants. One of sources of food that contain high antioxidants is purple sweet potato. ThiS research aims to determine the effect of giving purple potato-s extract to MDA levels on diabetic type 2 rat model. Rats were feed with proximate nutrition food of 20gr per day and drank 10% sucrose in ad libitum for 8 weeks and then injected with STZ multiple low dose intraperitoneal (IP) to become diabetic. Measurement of lipid peroxidation activity using the Thiobarbituric Acid (TBA) method. The result of this study is purple sweet potato has an effect to decrease malondialdehyde levels on diavetic type 2 rat model.

Keywords
Purple Sweet Potato, Malondialdehyde, DMT2

Topic
Biomedical Science

Link: https://ifory.id/abstract/cTWECdkK4RzY

Corresponding Author
Eka Pratama Putri

Institutions
1Departement of biology, Faculty of mathematics and Science, Universitas Negeri Malang, Semarang street 5, malang city 65112

Abstract
Diabetes mellitus is a disease characterized by chronic hyperglycemic conditions. Hyperglycemia condition can increase free radicals in the body. Excessive free radicals will be toxic and cause oxidative stress. Oxidative stress can be characterized by high lipid peroxidation activity and reactive oxygen species (ROS). Free radical activity can be prevented with antioxidants. The materials that contain strong antioxidants are black soybeans tempe and purple sweet potatoes. The antioxidants are isoflavones and anthocyanins. This study aimed to determine the effect of black soybean Tempe and purple sweet potato extract on reactive oxygen species (ROS) activity in liver DMT2 mode. In this study, mice were made diabetic with a high-fat diet, 10% sucrose drink and injected with multiple low-dose streptozotocin. The animals experiment were divided into 7 groups: Negative control (K-), Positive Control (K+), Treatment 1 (P1), Treatment 2 (P2), treatment 3 (P3), Treatment 4 (P4), Treatment 5 ( P5). Measurement ROS using ELISA method. The content of antioxidants of black soybean tempe and purple sweet potato extract can lower blood glucose levels and affects ROS activity.

Keywords
diabetes mellitus, reactive oxygen species, blood glucose

Topic
Biomedical Science

Link: https://ifory.id/abstract/yqXFtmQnJkPE

Corresponding Author
Sugeng Maryanto

Institutions
Nutrition Study Program, The University of Ngudi Waluyo
Diponegoro Street No. 186, East Ungaran, Semarang Regency, Central Java 50512, Indonesia
Email: sugengmaryanto99[at]gmail.com

Abstract
Modisco (Modified Dietetic Skimmed Milk and Coconut Oil) is a nutritious and high-calorie formula in the form of liquid food that was made from skim milk or full cream, sugar, and oil or margarine. The addition of soybean flour was given to increase the protein content which is intended for alternative handling cases of protein energy deficiency. This study was developed to analyze the effect of the modification of Modisco III formula by adding soybean flour to the growth of malnutrition rats. The design of this study was a pre-post test control group using 24 Wistar male rats which were treated malnutrition, which was divided into 4 groups. Control (-): low protein feed, Control (+): standard AIN-93G feed, Treatment (1): Modisco III soybean feed, and Treatment (2): Modisco III soybean meal + cassava flour. Based on the analysis of varians (ANOVA) there was a significant difference on the weight and body length between group (p <0.0001). The addition of soybean flour on the modified Modisco III had influenced on the weight and body length (p <0.0001) as well as on the growth of malnutrition rats.

Keywords
Modisco, soybean, malnutrition

Topic
Biomedical Science

Link: https://ifory.id/abstract/dkUfYhLjyWn8

Corresponding Author
Sugeng Maryanto

Institutions
1) Nutrition Study Program, Ngudi Waluyo University, Semarang, Indonesia
2) Departement Science of Nutrition, Faculty of medicine, Diponegoro University, Semarang – Indonesia
Email: dyahkartika[at]unw.ac.id

Abstract
Soaring drug prices and side effects for health have the effect of boosting food supplementation by utilizing natural resources. Increased consumption of phytochemicals that have antioxidant activity can suppress the onset of degenerative diseases. Therefore, it is necessary to prevent the improvement of food consumption patterns, one of which is natural antioxidants from papaya leaves. Proving the effect of giving jelly from extract papaya leaves to the level of SOD in healthy people. This research is an experimental study with a pre-post design randomized design involving 20 respondents consumption papaya leaves jelly within 20 days. Chlorophyll extraction was best found in extraction treatment with papaya leaf extract which had total chlorophyll content 11.29 mg/dL and antioxidant levels 52.13 mg/dL, taking into account the flavor and texture of jelly. Papaya leaf jelly has an effect on SOD levels (p = 0,000) with the meaning that it has a significant effect. Jelly from papaya leaf extraction 24.6 grams for 20 days gave a significant effect on increasing SOD levels in MDA levels in the blood.

Keywords
chlorophyll jelly, papaya leaves, antioxidants

Topic
Biomedical Science

Link: https://ifory.id/abstract/HNzWc4exqFTV

Corresponding Author
Mayvita Muntadiroh

Institutions
Faculty of Medicine University of Islam Malang
Address: Jl. MT. Haryono 193 Malang, East Java province, Indonesia 65145
*Correspondence: Dr. dr. Doti Wahyuningsih, M. Kes
E mail address: dotiwahyuningsih[at]unisma.ac.id

Abstract
Introduction: Osteoarthritis (OA) is a chronic degenerative disease associated with inflammatory process and free radical formation. It causes progressive joint cartilage damage. The rhizome of red ginger and cogon grass (RGCG) have anti-inflammatory and antioxidant properties. This present study aimed to evaluate the effects of RGCG treatment on nitrate level of plasmatic and synovial membrane tissue of Wistar rats. Materials and methods: Twenty five male Wistar rats were grouped into five; control (CTL), osteoarthritic rats (OA), and 3 different doses of treatment groups (P1, P2, P3). Each rat of OA and treatment groups were given 0,1 ml right knee intraarticular complete Freund-s adjuvant (CFA) injection to induce local osteoarthritic state. All treatment groups were orally administered with 40 mg/day of I. cylindrica decoction. However, P1, P2 and P3 groups were given 9, 18 and 36 mg of Z. officinale decoction each day for 10 days, respectively. Nitrate level was measured with nitric oxide colorimetric assay kit. Plasmatic nitrate data was analyzed by Kruskal-Wallis and continued with Mann-Whitney. However, nitrate level of synovial membrane tissue was using oneway ANOVA continued with Post Hoc. Result were considered to be significant at P<0,05. Results: Plasmatic nitrate level of treatment groups was 4.3 ± 0.55, 3.91 ± 2.39, and 3.64 ± 0.7 nmol/µL respectively in P1, P2, and P3. These were significantly lower than OA group (7.15 ± 2.68 nmol/µL, P<0,05). In addition, synovial membrane tissue nitrate level of treatment groups was 2.99 ± 1.18, 2.79 ± 1.09 and 2.87 ± 0.41 nmol/µL respectively in P1, P2, and P3. These were significantly lower than OA group (3.84 ± 1.56 nmol/µL, P<0,05). Conclusions: The administration of RGCG decoction reduced the level of nitrate both in blood plasma and synovial membrane tissue in osteoarthritic-induced Wistar rats. The combination of Zingiber officinale and Imperata cylindrica may have potential as an anti-osteoarthritis agent.

Keywords
osteoarthritis, Zingiber officinale, Imperata cylindrica, nitric oxide

Topic
Biomedical Science

Link: https://ifory.id/abstract/7ADzTwvKdmgP

Corresponding Author
Alif Rofiqotun Nurul Alimah

Institutions
Universitas Negeri Malang

Abstract
Single bulb garlic contains high sulfur compounds and widely used to reduce cholesterol. The imbalanced between Low Density Lipoprotein (LDL) and High Density Lipoprotein (HDL), increase of Triglyceride (TG) and total cholesterol will trigger various diseases including atherosclerosis. This study aimed to analyze the lipid profiles in mice fed a high-fat diet and treated with single bulb garlic extract. This research used twenty-four eight-week-old mice Balb/C grouped into six groups; normal diet (ND), high-fat diet (HFD), HFD with 26 mg/kg BW simvastatin (HFD + simv), HFD with single bulb garlic extract (SBGE) 125 mg/kg BW, 250 mg/kg BW, and 500 mg/kg BW respectively. Simvastatin and SBGE administrated daily for thirty day. At the end of the research, mice were sacrificed, then blood was collected from the heart and sentrifuged to obtain serum. Data were analyzed with one-way ANOVA. The results showed that administration of a SBGE was able to restore HDL and LDL concentration and reduce cholesterol and tryglyceride levels significantly (p<0.05) in mice with HFD. Our result suggest that SBGE could use as supplement or therapy to treat atherosclerosis.

Keywords
Single bulb garlic, high fat diet, lipid profile, atherosclerosis

Topic
Biomedical Science

Link: https://ifory.id/abstract/9nGrAFQL7ujN

Corresponding Author
Rahma Auri Wijayanti

Institutions
Faculty of Medicine, University of Islam Malang

Abstract
Introduction Osteoarthritis (OA) due to predispose and biomechanics factors leads to direct degradation of the cartilage. This degradation process breakdowns the lipopolysaccharide of macrophages which then increases leukotrienes- production and plasmatic leukocyte. The red ginger (Z. officinale) and cogon grass (I. cylindrica) rhizomes are known to have an anti-inflammatory and antioxidant properties. This present study evaluated the effects of Z. officinale and I. cylindrica decoction on plasmatic leukocytes and its differential count in osteoarthritic-induced Wistar rats. Materials and methods Twenty five male Wistar rats aged four months, weighed 270-300 g were grouped into five groups; control (CTL), osteoarthritic rats (OA), and 3 different doses of treatment groups (P1, P2, P3). Each rat of OA and treatment groups were given 0,1 ml right knee intraarticular complete Freund-s adjuvant (CFA) injection to induce local osteoarthritic state. All treatment groups were orally administered with 40 mg/day of I. cylindrica decoction. However, P1, P2 and P3 groups were given 9, 18 and 36 mg of Z. officinale decoction each day for 10 days, respectively. Plasmatic leukocytes and its differential count were measured using hematology analyzer. Data were statistical analyzed with One Way ANOVA test followed by post hoc test. The significant level at P less than 0.05. Results The plasmatic leucocytes was 11.46 ± 7.72, 53.84 ± 22.39, 4.44 ± 1.04, 3.76 ± 0.35, and 8.94 ± 4.66 respectively in CTL, OA, P1, P2, and P3 (103/µL). The treatment groups have lower plasmatic leucocytes in comparison with OA group (P <0.05). We then evaluated the differential types of leucocyte; granulocyte, lymphocyte and monocyte. Plasmatic granulocyte was 2.03 ± 1.42, 13.7 ± 9.05, 0.21 ± 0.08, 0.47 ± 0.74, and 0.95 ± 1.11 respectively in CTL, OA, P1, P2, and P3 (103/µL). Plasmatic lymphocyte was 7.88 ± 5.43, 30 ±13.03, 3.14 ± 0.62, 2.9 ±0.34 and 6.6 ± 3.95 respectively in CTL, OA, P1, P2, and P3 (103/µL). Plasmatic monocyte was 1.72 ± 0.78, 8.12 ± 4.14, 1.08 ± 0.43, 0.74 ± 0.13 and 1.38 ± 0.63 respectively in CTL, OA, P1, P2, and P3 (103/µL). The treatment groups have lower differential count of leucocytes in comparison with OA group (P <0.05). Conclusions Decoction extract of Z officinale and I cylindrica reduced plasmatic leukocytes and corrected its differential count in osteoarthritic-induced Wistar rats. Combination of Z officinale and I cylindrica may have potential as anti-osteoarthritis. Keywords: Zingiber officinale, Imperata cylindrica, leucocytes, osteoarthritis *Correspondence: Dr. dr. Doti Wahyuningsih, M. Kes Faculty of Medicine University of Islam Malang Address: Jl. MT. Haryono 193 Malang, East Java province, Indonesia 65145 E mail address: dotiwahyuningsih@unisma.ac.id *Correspondence: dr.Rima Zakiyah,Sp.Rad Faculty of Medicine Uniersity of Islam Malang Address : Perum. Galxy Regency,Malang, East Java province,Indonesia

Keywords
Zingiber officinale, Imperata cylindrica, leucocytes, osteoarthritis

Topic
Biomedical Science

Link: https://ifory.id/abstract/aDthL2bTEMcZ

Corresponding Author
Eko Susetyorini

Institutions
Departement of Biology Education, Universitas Muhammadiyah Malang, Jl. Raya Tlogomas No. 246 Malang, East Java, Indonesia (62341) 551149

Abstract
Based on the substances it contains, kaffir lime leaves (Citrus hystrix DC) are indicated to have efficacy in increasing female reproductive fertility. The purpose of this study was to examine the effect of kaffir lime leaf extract on the number of ovarian follicles of female white rats (Rattus norvegicus). This true-experimental research used three levels of extract concentration, i.e. 250 mg / kg, 500 mg / kg, and 750 mg / kg. Data from the study were analyzed using one-way ANOVA and continued with DMRT test. As a result, the administration of kaffir lime leaf extract had a significant effect on ovarian follicles number in white rats (p < 0.001). Treatment with a dose of 500 mg/kg and 750mg/kg did not have a significant difference in the number of primary, tertiary and de graff follicles, while the secondary follicles in the treatment of 750 mg/kg had a significantly higher number of other treatments. Therefore, kaffir lime leaf extract has the potential as herbal medicine for womens reproductive health.

Keywords
Citrus hystrix, female fertility, ovarian follicle

Topic
Biomedical Science

Link: https://ifory.id/abstract/RgWBhC9zmxwc

Corresponding Author
Nursasi Handayani

Institutions
Jurusan Biologi Universitas Negeri Malang

Abstract
The fingerroot (Kaempferia pandurata ROXB) is one of the medicinal plants. Beside as a spice, based on research, the temu kunci plant can be used as a medicine. Fingerroot contain pivotal compounds such as essential oils, starch, resin, tannins, saponins, flavonoids, pinostrolerin, and alipinetin. Mostly, the plants containing saponins, flavonoids and phenols have the effect of spermicide or sperm immobilization. Based on this issue, the use of fingerroot rhizomes can have an impact on the reproductive organs. Until now there is still little research on the effects fingerroot rhizome on reproductive organs, especially as antifertility. The use of natural ingredients as antifertility has smaller side effects than synthetic chemicals. The aims of this study to determine the effect of decoction of fingerroot rhizome simplicia on the quality of male mice sperm. In this present study, the concentration that were utilized are 0%, 5%, 7.5%, 10%, 12.5%, and 15 %. The results showed that the fingerroot rhizome simplicia decoction had an effect on the testis of Balb C mice (Mus musculus).

Keywords
fingerroot rhizome, antifertility, mice

Topic
Biomedical Science

Link: https://ifory.id/abstract/C72mufQdYMav

Corresponding Author
Husnul Khotimah

Institutions
1State Health Polytechnic of Health Ministry Malang, Malang, Indonesia
2Department of Obstetri and Gynecology, Saiful Anwar Hospital, Medical Faculty Brawijaya University, Indonesia
3Biochemistry Laboratory, Faculty of Science, Brawijaya University, Indonesia
4Veterinary Medicine School, Brawijaya University, Indonesia
5Department of Pharmacology, Medical Faculty, Brawijaya University, Indonesia

Abstract
Cervical carcinoma is the second cancer type that common in woman and the most caused of woman death all over the world. Human Papilloma Virus (HPV) infection suggested could induced gene hypemethylation. Hypermethylation that occur on p16 tumor suppressor gene promoter resulted in silencing tumor suppressor gene so that the gene is inactive and contribute to malignancy of cancer cells. Tea paracytes or Loranthaceae known to be a plant that have an anticancer potency, correlated to its flavonoids content. Flavonoid from Scurrula atropurpurea (Blume) Danser (SAD) suggested have a role in the inhibiting methylation towards p16. This research objective was to identify the potency of Lawang - East Java Scurrula atropurpurea (Blume) Danser (SAD) flavonoids compound towards Hela cells. In this study, we examine flavonoids from SAD that been extracted in n-heksan, chloroform, etanol solvent, using TLC Chromatogram and LCMS. We also examined the cells proliferation using MTT assay, apoptotic level was examine by Tunnel, and then completed with methylation level detection using bisulfit direct sequencing method. The result shown that flavonoids that found were : flavanon, dihidroflavonol and flavon; dihidroflavonol, flavanon and cathecin, and flavonol, flavon, and EGCG, respectively.in n hexane, chloroform and ethanol fraction. Flavonoids that were found suggested to have proliferation inhibitor and apoptotic inductor potency via inhibition of p16 promoter of HeLa cells methylation. SAD flavonoids in the n-heksan and chloroform fraction shown the most potent in methylation inhibition. We suggested that combination of flavanon and dihidroflavonol have an important role in p16 demethylation. SAD flavonoids had activity to affect the methylation of p16 and bioactive compound could be one of a novel agent that have to be explore in the future as a demethylating agent in cancer.

Keywords
Methylation, Apoptotic, Proliferation, p16, Scurrula atropurpurea (Blume) Danser

Topic
Biomedical Science

Link: https://ifory.id/abstract/CjWEHZqKU7ha

Corresponding Author
Siti Nur Arifah

Institutions
Universitas Negeri Malang

Abstract
Atherosclerosis is an inflammatory of artery wall by lipoprotein oxidation. Food with high content of fat is one of causes increases the level of LDL. The increase in LDL level increases free radicals in form of ROS and causing to be easily oxidized into Ox-LDL. Ox-LDL triggers the activation of macrophage and Th17 cells to secrete pro-inflammatory cytokine IL-6. Treatment for atherosclerosis uses simvastatin could influence the activity of nervous system, so that safe treatment with herbal treatment, one of examples is single bulb garlic. The research aims to find out the influence of single bulb garlic oil on the decrease ROS thus it could inhibit IL-6 secretion and the number of lymphocytes in mice with high fat diet (HFD). Mice were feed with high fat diet for 45 days and treated for 30 days. Mice were grouped into 6 groups, normal, HFD with simvastatin 26 mg/kg BW, single bulb garlic oil 0 mg/kg BW, 12.5 mg/kg BW, 25 mg/kg BW, and 50 mg/kg BW. In the end of treatment, mice were dissected for collection of (1) serum in order to measure its level using ELISA with ROS and IL-6 antibodies, (2) aorta from heart to measure its expression using IHC-F with IL-6 antibody, lymphocyte from bone marrow and spleen was calculated for the number of cells using light microscope. The result indicated that the single bulb garlic oil has influence on decrease of ROS level, IL-6 level, IL-6 expression and the number of lymphocyte.

Keywords
single bulb garlic oil, ROS, IL-6, lymphocyte

Topic
Biomedical Science

Link: https://ifory.id/abstract/nRTpKAWEewUV

Corresponding Author
Ihsan Budi Rachman

Institutions
1,2,3,4Department of Chemistry, Faculty of Mathematics and Natural Sciences, State University of Malang
5Institute of Bioscience, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia

Abstract
Vanillin is one of the compounds that was successfully isolated from the fruits of the Vanilla planifolia Andrews which are abundant in Indonesia. So far, Indonesia has only been able to export vanilla fruit directly without further processing. The IUPAC name of Vanillin is 4-hydroxy-3-methoxybenzaldehyde. This compound is a derivative of benzaldehyde. The presence of an aldehyde group causes vanillin to experience a Knoevenagel condensation reaction if it is reacted with dimedone. In this paper, two derivatives of vanillin compounds successfully synthesized. The first compound has a pyran skeleton and the second compound has a pyridine skeleton. Both of these compounds have the potential as antibacterial agents against gram positive and negative bacteria.

Keywords
vanillin derivatives, Knoevenagel condensation, antibacterial agents

Topic
Biomedical Science

Link: https://ifory.id/abstract/euA7yvCtHfQD

Corresponding Author
Alfonds Andrew Maramis

Institutions
1Biology Education Study Program, Faculty of Mathematics and Nature Sciences, State Universiti of Manado (UNIMA), Campus of UNIMA at Tondano, Minahasa, 95618, North Sulawesi, Indonesia.
2Biology Study Program, Faculty of Mathematics and Nature Sciences, State Universiti of Manado (UNIMA), Campus of UNIMA at Tondano, Minahasa, 95618, North Sulawesi, Indonesia.

Abstract
Caspase-8 and TNFα are cellular molecules that play a role in apoptosis through the death receptor pathway. In connection with the study of toxification of formalin-containing foodfoodstuff and its detoxification using chlorophyllin, our research has previously proven that chlorophyllin supplementation was able to reduce the activation of Caspase-8 and the expression of TNFα which increases due to repeated exposure to formalin-containing foodstuffs. However, information about how significant the two molecules affect the initiation of apoptosis through the flow of caspase activation in the context of this toxicity is not clearly known. This study aims to determine the ratios of Caspase-8 and TNFα of liver cells of mice that experienced toxification of formalin and detoxification of chlorophyllin. Caspase-8 and TNFα of liver cells of mice that underwent the toxic-detoxification treatment were determined through immunohistochemical techniques. The ratio data of the two moleculs was analyzed descriptively and qualitatively. The results showed that the Caspase-8 and TNFα ratios varied with the toxicity-detoxification treatment, with a total ratio of 1.01.

Keywords
Caspase-8, TNFα, Formalin-containing Foodstuff, Chlorophyllin.

Topic
Biomedical Science

Link: https://ifory.id/abstract/BkCXKxqd2LWM

Corresponding Author
Umi Latifah

Institutions
1Generasi Biologi Indonesia (Genbinesia) Foundation, Jl. Swadaya Barat No. 4, Semampir Cerme Gresik Regency, 61171, East Java, Indonesia
2Department of Parasitology, Faculty of Medicine, Community Health and Nursery Gadjah Mada University
3Department of Internal Disease Faculty of Medicine, Community Health and Nursery Gadjah Mada University

Abstract
Abstract. Intestine parasites were one of the causes behind the occurrence of intestinal disorder such as diarrhea. The risk of being infected by the intestine parasites increased among the HIV patients due to the weakening immunity, as a result, the intestinal parasites would easily infect the patients. This study was conducted to determine the prevalence, to identify the relationship between the body immunity level and the intestinal parasitic infection among HIV patients in Dr.Sardjito Central Hospital Yogyakarta. Out of 75 samples of faeces HIV patients collected from July 2017 until January 2018. Intestinal parasites were identify using Ziehl Neelsen painting and Ritchie concentration method. The data were analysed using chi-square test with p-value that had been higher than 0,05. The intestine parasites that had been found in the research were namely Cryptosporidium parvum, Isospora belii, Entamoeba histolytica, Entamoeba coli, Balantidum coli, lodomoeba butschlii, Ascaris lumbricoides, Trichuris trichiura, Hookworm, and Hymenolepis nana. There had not been significant relationship between the intestinal parasitic infection and the immunity status of HIV patients. The prevalence of intestinal protozoa among the HIV patients was 52 (69.3%), while the prevalence of intestinal worm was 6 (8%).

Keywords
HIV patients, CD4+ level, intestinal parasitic infection.

Topic
Biomedical Science

Link: https://ifory.id/abstract/Fh9pPBVeuy3D

Corresponding Author
Fendy Hardian Permana

Institutions
Department of Biology Education.
Faculty of Teacher Training and Education.
Universitas Muhammadiyah Malang

Abstract
One of the biological agents that have many benefits is papaya seed (Carica papaya). On the other hand, papaya seeds also contain some chemical compounds that have toxic properties such as papain and karpain. The aim of this study was to examine the acute toxicity of papaya seed extract by looking at the mortality rates in the population and reviewing the physiological effects manifested by its behavior. Behavior that measured such as the general behavior, aggressiveness and tail movement. The method used to test acute toxicity is by LD50 Miller and Stainter method then categorized its toxicity threshold. Then the emerging physiological impact was analyzed by Likert scale and given the score. After that the data is tested with normality test and one way anova. Toxicity test showed that LD50 extract of papaya seed extract was 18.62 g / kg BW and categorized as practically non toxic. Then there is the effect of papaya seed extract on behavioral physiological and aggressiveness. There was no effect of various doses of papaya seed extract on mouse tail movements

Keywords
Toxicity of papaya seed; Wistar white rat

Topic
Biomedical Science

Link: https://ifory.id/abstract/tnhGCzpvjg73

Corresponding Author
Ihsan Budi Rachman

Institutions
1Chemistry Department Faculty of Mathematics and Natural Sciences Universitas Negeri Malang
Jl. Semarang No. 5 Malang 65341

a) Corresponding author: sutrisno.kimia[at]um.ac.id

Abstract
Sesame seed oil is a vegetable oil sourced from sesame seed extract. Sesame seed oil is rich with unsaturated fatty acids, especially oleic acid (C18:1 9c) and linoleic acid (C18:2 9c12c, ω-6). Sesame seed oil has not been widely utilized in the field of pharmacy, because it is expensive and more used for cooking. Previous studies show that sesame seed oil had no antibacterial activity and was acquired the fatty acid constituent components of sesame seed oil. The research aims are: (1) Transformation sesame seed oil into its derivatives, (2) characterization the sesame seed oil derivative products (3) identification of the product of sesame seed oil and (4) evaluation of antibacterial activity of its derivatives. The research consisted of 5 stages. Stage 1: characterization and identification of sesame seed oil which includes appearance, color, density, refractive index, viscosity, solubility test, acid number, number of conformations, ester number, IR spectrum interpretation and GC-MS. Stage 2: synthesis, characterization and identification which includes the appearance, color, melting point, solubility test and IR spectrum interpretation of potassium soap. Stage 3: synthesis, characterization and identification which include form, color, density, refractive index, viscosity, boiling point, solubility test, acid number, number of conformations, ester number and IR spectrum interpretation of fatty acids. Stage 4: transesterification of sesame seed oil, characterization and identification which include appearance, color, density, refractive index, viscosity, solubility test, acid number, number of conformations, ester number, IR spectrum interpretation and GC-MS. Stage 5: evaluation of antibacterial activity to Staphylococcus aureus and Escherchia coli bacteria. The saponification of sesame seed oil with potassium hydroxide produced potassium soap with solid tangible yellowish white appearance and has a melting point 235 – 240 oC. Acidification potassium soap with hydrochloric acid was obtained a yellowish liquid fatty acid, density of 0.93 g.mL-1, refractive index 1.46 (25 º C), viscosity 60.54 cSt, acid number 140.55, saponification number 165.55, and ester number 25.00. Transesterification of sesame seed oil using methanol catalyzed potassium hydroxide produced methyl ester liquid fatty acids yellow colored, density of 0.89 g.mL-1, refractive index 1.45 (25 º C), viscosity 35.06 cSt, acid number 0.56, saponification number 429.21, and ester 428.65. Potassium soap (2% and 1%), fatty acids (2% and 1%), and methyl ester is 2% were active as an antibacterial agent to Escherchia coli and Staphylococcus aureus bacteria with medium category.

Keywords
triglyceride derivatives, sesame seeds oil, antibacterial

Topic
Biomedical Science

Link: https://ifory.id/abstract/YU8pKQHvqgGJ

Corresponding Author
Retty Ratnawati

Institutions
1) Faculty of Medicine, Universitas Brawijaya, Malang
2) Dept. of Chemistry, Faculty of Science, Institute Technology Bandung

Abstract
Brain injury caused by physical trauma in our TBI (trauma brain injury) rat model, have been approved to recover after administered by total catechins derived from green tea. The improvement have been detected in the increase of the neurological severity score (NSS). While in the molecular level, the improvement could be detected through the decrease of TNF alpha, caspase 8, caspase 3, NFKappa B p65 and also the increase of the BDNF and ratio BCl2/Bax. Hyperglycemia could damage the brain that can be seen in our memory spatial rat model. The administered of the total anthocyanin derived from sweet purple potatoes Ipomoea batatas L. kultivar Gunung Kawi, was used to these animals to improve the memory spatial of the rat. It can be determined through the molecular level, such as the decrease of the MDA, Caspase 3, TNF alpha and also the decrease of the travel time Morris water maze However there is still problems to decide the exact rationale doses of these two natural anti-oxidant. Therefore, it is a deemed to continue to elaborate the doses.

Keywords
trauma brain injury, memory spatial, total catechins, total anthocyanin sweet potato

Topic
Biomedical Science

Link: https://ifory.id/abstract/xFjMWaBQgpE9